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From Proteopedia
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Rho Transcription Termination Factor (3ICE)
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Rho Transcription Termination Factor is a 7 chain structure that is found in E.coli. The molecule facilitates termination of transcription by binding to the nascent RNA via ATP hydrolysis. This protein is found in rho-dependent prokaryotes (there are rho-independent prokaryotes that have their own ways of terminating transcription.)
Structure and Cellular Interaction
Rho termination factor is made up of four ligands: , , , and ADP.
The RNA strand itself has a sequence upstream of the termination sequence called the rho utilization site (rut); it is rich in cytosine and is the sequence to which the rho factor binds to. Once bound to the RNA strand, the factor moves from 5'-3' downstream towards the RNA polymerase and its transcription bubble. Once the RNA polymerase reaches the termination sequence on the DNA, it will pause, allowing the Rho factor to catch up. Once catching up, the factor has helicase activity, causing separation of the RNA strand from the DNA template.
Video
https://www.youtube.com/watch?v=cxhXCsvHVuI
References
Pierce, Benjamin A. Genetics: A Conceptual Approach. New York: W.H. Freeman, 2005. Print.
"Rho factor." Wikipedia. Wikimedia Foundation, n.d. Web. 08 Feb. 2017.
"3ice." 3ice - Proteopedia, life in 3D. N.p., n.d. Web. 08 Feb. 2017.
