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From Proteopedia
Contents |
CO-CRYSTAL STRUCTURE OF XENOPUS TFIIIA ZINC FINGER DOMAIN BOUND TO THE 5S RIBOSOMAL RINA GENE INTERNAL CONTROL REGION
This is present in the organism Xenopus laevis (African clawed frog) and is a folded region of protein, characterized by the Zinc that stabilized the folds present. This is a specific zinc finger found in a specific region of the protein Transcription Factor IIIA
Function and Interactions
This specific zinc finger serves two purposes: it is a positive transcription factor for 5S RNA genes and it is also a specific RNA binding protein that forms a complex with 5S RNA in oocytes, creating the 7S ribonucleoprotein storage particle. It also might play an important role in the developmental processes undergone in the expression of the 5S RNA gene. It interacts with the internal control region of 50 different bases located on the 5S RNA genes, and is vital for accurate transcription of these genes by RNA polymerase III. Additionally, it binds the transcribed 5S RNA's.
Structure and Scenes
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The specific that constitutes TFIIIA relies on numerous specific pieces in order to achieve its function. across the molecule contribute to the stabilizing forces that hold the intricate folds of the RNA in place. The TFIIIA is currently engaged with 2 molecules of , one in both Biological Assembly 1 and Biological Assembly 2.
Evolutionary Background
Various regions of this molecule appear to have a differing level of evolutionary conservation in coevolutionary developments with corresponding DNA and RNA molecules, with which the zinc finger proteins interact by recognizing different sets of nitrogenous bases.
References
Nolte RT, Conlin RM, Harrison SC, Brown RS. Differing roles for zinc fingers in DNA recognition: structure of a six-finger transcription factor IIIA complex. Proc Natl Acad Sci U S A. 1998 Mar 17;95(6):2938-43. PMID:9501194
RCSB Protein Data Bank, Nolte, R.T., Conlin, R.M., Harrison, S.C., Brown, R.S. (n.d.). 1TF6. Retrieved February 08, 2017, from http://www.rcsb.org/pdb/explore.do?structureId=1tf6
European Bioinformatics InstituteProtein Information ResourceSIB Swiss Institute of Bioinformatics. (2017, January 18). Transcription factor IIIA. Retrieved February 08, 2017, from http://www.uniprot.org/uniprot/P03001
Lu, D., Searles M.A., Klug, A. Crystal structure of a zinc-finger–RNA complex reveals two modes of molecular recognition. Nature 426, 96-100 (6 November 2003. Retrieved February 08, 2017, from http://www.nature.com/nature/journal/v426/n6962/full/nature02088.html
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