This is the overall . The membrane access channel is accessible to lipids partitioned in the inner layer of the lipid bilayer. This is the . This is the .
The of FAAH reveals two equivalent openings (, ) directly accessible by the inner layer of the lipid bilayer.[1] These (MAC) are made up of three flaps and two intrusions which collectively form the entry way for the aliphatic binding of the amide lipid substrate. Flaps 1 and 2 envelope the middle and backside of the anandamide mimic, and are locked together by a between Arg486 and Asp403. Flap 2 contains a very that partially covers the opening with Phe432. This binding cap clings to the cell's hydrophobic inner membrane and uses a multitude of to lure out partitioned anandamide by its narrow partial negative charge. The catalytic site is defined by the catalytic triad: the contributes the nucleophilic S241, loop 3 contributes the neighboring upon forming the very top of the membrane access channel, and a fourth loop contributes the K142.
The membrane access channel leads to the active site, which is flanked by both the (ABP and CP).[2] The cytosolic port is a lengthy, flexible loop that leads directly into the cytoplasm, allowing the deacylated amine to enter the cell.
