8wb2
From Proteopedia
Heme-bound Arabidopsis thaliana temperature-induced lipocalin
Structural highlights
FunctionTIL_ARATH Involved in basal (BT) and acquired thermotolerance (AT), probably by preventing plasma membrane lipids peroxidation induced by severe heat-shock (HS) (PubMed:19302169, PubMed:23837879). Lipocalin that confers protection against oxidative stress caused by heat, freezing, paraquat and light (PubMed:18671872, PubMed:19302169). Confers resistance to high salt (NaCl) levels, probably by protecting chloroplasts from ion toxicity via ion homeostasis maintenance (PubMed:20959419, PubMed:24028869). Required for seed longevity by ensuring polyunsaturated lipids integrity (PubMed:23837879).[1] [2] [3] [4] [5] Publication Abstract from PubMedArabidopsis thaliana temperature-induced lipocalin (AtTIL) is a prototypical member of plant lipocalins and participates in a variety of cellular processes, particularly stress responses. Bioinformatical and physiological studies have proposed its promiscuous ligand-binding ability, but the molecular basis is yet unclear. Here, we report the 1.9-A crystal structure of AtTIL in complex with heme. Spectrophotometric absorbance titration with heme yields a dissociation constant of approximately 2 micromolar, indicating the relatively weak interaction between AtTIL and heme, which is confirmed by the AtTIL-heme structure. Although binding to retinal or biliverdin is not detected, such possibility can not be precluded as suggested by comparison with other lipocalin structures. These results show that AtTIL is a structural and functional homolog of the bacterial lipocalin Blc. Crystallographic and functional studies of a plant temperature-induced lipocalin.,Dong CS, Zhang WL, Wang XY, Wang X, Wang J, Wang M, Fang Y, Liu L Biochim Biophys Acta Gen Subj. 2023 Dec 14;1868(2):130540. doi: , 10.1016/j.bbagen.2023.130540. PMID:38103756[6] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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