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Talk:CRISPR-Cas9
From Proteopedia
List of figures: CRISPR-Cas9
--Karsten Theis 01:51, 20 July 2019 (UTC)
Initial view
script: https://proteopedia.org/wiki/scripts/74/742625/Cv3/13.spt
coords: https://proteopedia.org/cgi-bin/getfrozenstructure?14e7e6b27144899555581f95781095d3 (always reloads)
Domain organization of nuclease lobe of Cas9 from S. pyogenes
script: https://proteopedia.org/wiki/scripts/74/746096/Cv3/1.spt
coords: same as Initial view (always reloads)
crRNA and tracrRNA can be joined end-to-end and transcribed as a single guide RNA (sgRNA)
script: https://proteopedia.org/wiki/scripts/74/742625/Cv3/8.spt
coords: https://proteopedia.org/cgi-bin/getfrozenstructure?390674a1fe8f149d2a471a66257db804 (always reloads)
Cas9-sgRNA-target DNA complex from Streptococcus pyogenes
script: https://proteopedia.org/wiki/scripts/74/742625/Cv/42.spt
coords: https://proteopedia.org/cgi-bin/getfrozenstructure?24390165f6e745806f0ed99c67de2118 (always reloads)
Cas9-sgRNA-target DNA complex from Streptococcus pyogenes
script: https://proteopedia.org/wiki/scripts/74/742625/Cv2/12.spt
coords: https://proteopedia.org/cgi-bin/getfrozenstructure?9dbb58242e693852cd408648accf59ea (always reloads)
Cas9-sgRNA-target DNA complex from Francisella tularensis
script: https://proteopedia.org/wiki/scripts/74/742625/Cv2/13.spt
coords: https://proteopedia.org/cgi-bin/getfrozenstructure?0a7d3ac27b1d671cca434c9eff24c49c (always reloads)
Cas9-sgRNA-target DNA complex from Staphylococcus aureus
script: https://proteopedia.org/wiki/scripts/74/742625/Cv3/2.spt
coords: https://proteopedia.org/cgi-bin/getfrozenstructure?2ccede112780a94592edf96642f32d01 (always reloads)
optimal DNA target of the complex is determined by a Watson–Crick base pairing of a short ∼20-nt sequence within sgRNA (within the crRNA in wild-type)
script: https://proteopedia.org/wiki/scripts/74/742625/Cv3/4.spt
coords: https://proteopedia.org/cgi-bin/getfrozenstructure?e44061e874ac35d8806b45eaf9f9ebaf (always reloads)
few nucleotide long conserved motif recognized directly by Cas9 protein (protospacer adjacent motif, PAM)
script: https://proteopedia.org/wiki/scripts/74/742625/Cv3/10.spt
coords: same as optimal DNA target of the complex is determined by a Watson–Crick base pairing of a short ∼20-nt sequence within sgRNA (within the crRNA in wild-type) (always reloads)
few mismatches between guide sequence and target DNA can be tolerated
script: https://proteopedia.org/wiki/scripts/74/742625/Cv/44.spt
coords: https://proteopedia.org/cgi-bin/getfrozenstructure?3d79d333a058bb5bd2af9650e145ebe1 (always reloads)
When Cas9 is in the apo state, its PAM-interacting cleft is largely disordered
script: https://proteopedia.org/wiki/scripts/74/742625/Cv4/3.spt
coords: https://proteopedia.org/cgi-bin/getfrozenstructure?ce8514cc5aaf7818a31c1779fc0adf71 (always reloads)
Pre-target state of CAS9 with sgRNA
script: https://proteopedia.org/wiki/scripts/74/742625/Cv4/4.spt
coords: same as When Cas9 is in the apo state, its PAM-interacting cleft is largely disordered (always reloads)
CAS9 with sgRNA and tagret DNA
script: https://proteopedia.org/wiki/scripts/74/742625/Cv4/5.spt
coords: same as When Cas9 is in the apo state, its PAM-interacting cleft is largely disordered (always reloads)
Animation of these scenes
script: https://proteopedia.org/wiki/scripts/74/742625/Cv4/7.spt
coords: same as When Cas9 is in the apo state, its PAM-interacting cleft is largely disordered (always reloads)
Cas9-sgRNA-dsDNA complex
script: https://proteopedia.org/wiki/scripts/74/742625/Cv4/11.spt
coords: https://proteopedia.org/cgi-bin/getfrozenstructure?e9eee6ce3083b7b447d2efbbf956be6c (always reloads)
sgRNA-dsDNA without protein is shown
script: https://proteopedia.org/wiki/scripts/74/742625/Cv4/12.spt
coords: same as Cas9-sgRNA-dsDNA complex (always reloads)
Scissile phosphates
script: https://proteopedia.org/wiki/scripts/74/742625/Cv4/14.spt
coords: same as Cas9-sgRNA-dsDNA complex (always reloads)
Cas9 interacts with both separated DNA strands
script: https://proteopedia.org/wiki/scripts/74/742625/Cv4/15.spt
coords: same as Cas9-sgRNA-dsDNA complex (always reloads)
deactivated Cas9 (dCas9)
script: https://proteopedia.org/wiki/scripts/74/742625/Cv4/8.spt
coords: same as crRNA and tracrRNA can be joined end-to-end and transcribed as a single guide RNA (sgRNA) (always reloads)
mutating two key residues within its nuclease domains
script: https://proteopedia.org/wiki/scripts/74/742625/Cv4/9.spt
coords: same as crRNA and tracrRNA can be joined end-to-end and transcribed as a single guide RNA (sgRNA) (always reloads)
Domain organization of nuclease lobe of Cas9 from S. pyogenes
script: https://proteopedia.org/wiki/scripts/74/746096/Cv3/1.spt
coords: same as Initial view (always reloads)
Cas9-sgRNA-target DNA complex from Streptococcus pyogenes
script: https://proteopedia.org/wiki/scripts/74/742625/Cv/42.spt
coords: same as Cas9-sgRNA-target DNA complex from Streptococcus pyogenes (always reloads)
Cas9-sgRNA-target DNA complex from Streptococcus pyogenes
script: https://proteopedia.org/wiki/scripts/74/742625/Cv2/12.spt
coords: same as Cas9-sgRNA-target DNA complex from Streptococcus pyogenes (always reloads)
CAS9 with sgRNA and tagret DNA
script: https://proteopedia.org/wiki/scripts/74/742625/Cv4/5.spt
coords: same as When Cas9 is in the apo state, its PAM-interacting cleft is largely disordered (always reloads)
Cas9-sgRNA-dsDNA complex
script: https://proteopedia.org/wiki/scripts/74/742625/Cv4/11.spt
coords: same as Cas9-sgRNA-dsDNA complex (always reloads)
Jmol commands: buttons etc
Summary
Total of green links: 25
Total of Jmol buttons etc: 0
coordinates used
- /cgi-bin/getfrozenstructure?14e7e6b27144899555581f95781095d3
- /cgi-bin/getfrozenstructure?390674a1fe8f149d2a471a66257db804
- /cgi-bin/getfrozenstructure?24390165f6e745806f0ed99c67de2118
- /cgi-bin/getfrozenstructure?9dbb58242e693852cd408648accf59ea
- /cgi-bin/getfrozenstructure?0a7d3ac27b1d671cca434c9eff24c49c
- /cgi-bin/getfrozenstructure?2ccede112780a94592edf96642f32d01
- /cgi-bin/getfrozenstructure?e44061e874ac35d8806b45eaf9f9ebaf
- /cgi-bin/getfrozenstructure?3d79d333a058bb5bd2af9650e145ebe1
- /cgi-bin/getfrozenstructure?ce8514cc5aaf7818a31c1779fc0adf71
- /cgi-bin/getfrozenstructure?e9eee6ce3083b7b447d2efbbf956be6c
