RNA Interference
From Proteopedia
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| - | [[Image:500px-ShRNA Lentivirus.png|350px|left|thumb| Schematic of RNAi Interference in Mammalian Cells]] | ||
{{Structure | {{Structure | ||
|PDB= 3dlh |SIZE=300|SCENE=RNA_Interference/3dlh/2|CAPTION= guide-strand-containing Argonaute protein silencing complex ([[3dlh]]), resolution 3.00Å (<scene name='RNA_Interference/3dlh/2'>initial scene</scene>) | |PDB= 3dlh |SIZE=300|SCENE=RNA_Interference/3dlh/2|CAPTION= guide-strand-containing Argonaute protein silencing complex ([[3dlh]]), resolution 3.00Å (<scene name='RNA_Interference/3dlh/2'>initial scene</scene>) | ||
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}} | }} | ||
[[RNA Interference]] (RNAi) is a system in living cells that helps control the level of activity of genes. In RNAi, a long double stranded [[RNA]] is cleaved into shorter strands. One strand known as the guide strand is complimentary to the mRNA gene product of a gene of interest. This guide strand is incorporated into an RNA-induced silencing complex (RISC) and subsequently binds the mRNA of the gene of interest. After binding, the RNA/RISC complex subsequently silences the mRNA of interest via cleavage of the mRNA strand. | [[RNA Interference]] (RNAi) is a system in living cells that helps control the level of activity of genes. In RNAi, a long double stranded [[RNA]] is cleaved into shorter strands. One strand known as the guide strand is complimentary to the mRNA gene product of a gene of interest. This guide strand is incorporated into an RNA-induced silencing complex (RISC) and subsequently binds the mRNA of the gene of interest. After binding, the RNA/RISC complex subsequently silences the mRNA of interest via cleavage of the mRNA strand. | ||
| + | [[Image:500px-ShRNA Lentivirus.png|350px|left|thumb| Schematic of RNAi Interference in Mammalian Cells]] | ||
{{Structure | {{Structure | ||
|PDB= 1rpu |SIZE=300|ALIGN='left'|SCENE=User:Wayne_Decatur/Plant_Viral_Protein_p19_Suppression_of_RNA_Silencing/View1/2|CAPTION= viral silencing suppressor bound to a siRNA ([[1rpu]]), resolution 2.50Å (<scene name='User:Wayne_Decatur/Plant_Viral_Protein_p19_Suppression_of_RNA_Silencing/View1/2'>initial scene</scene>) | |PDB= 1rpu |SIZE=300|ALIGN='left'|SCENE=User:Wayne_Decatur/Plant_Viral_Protein_p19_Suppression_of_RNA_Silencing/View1/2|CAPTION= viral silencing suppressor bound to a siRNA ([[1rpu]]), resolution 2.50Å (<scene name='User:Wayne_Decatur/Plant_Viral_Protein_p19_Suppression_of_RNA_Silencing/View1/2'>initial scene</scene>) | ||
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Articles in Proteopedia concerning RNA Interference include: | Articles in Proteopedia concerning RNA Interference include: | ||
| - | *[[Suppression of RNA Silencing by Viruses|Suppression of RNA Silencing by Viruses]] | + | **[[Suppression of RNA Silencing by Viruses|Suppression of RNA Silencing by Viruses]] |
| - | *[[Plant_Viral_Protein_p19_Suppression_of_RNA_Silencing|Plant Viral Protein p19 Supression of RNA Silencing]] | + | **[[Plant_Viral_Protein_p19_Suppression_of_RNA_Silencing|Plant Viral Protein p19 Supression of RNA Silencing]] |
| - | *[[Tomato aspermy virus protein 2b Suppression of RNA Silencing|Tomato aspermy virus protein 2b Suppression of RNA Silencing]] | + | **[[Tomato aspermy virus protein 2b Suppression of RNA Silencing|Tomato aspermy virus protein 2b Suppression of RNA Silencing]] |
| - | *[[Flock house virus B2 protein Suppression of RNA Silencing|Flock house virus B2 protein Suppression of RNA Silencing]] | + | **[[Flock house virus B2 protein Suppression of RNA Silencing|Flock house virus B2 protein Suppression of RNA Silencing]] |
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| + | See RNAi in action by viewing this | ||
| + | [http://www.nature.com/nrg/multimedia/rnai/index.html RNAi Animation]. | ||
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| - | To view automatically seeded indices concerning RNA Interference | + | To view automatically seeded indices concerning RNA Interference, see: |
| - | *[[:Category:Argonaute|Argonaute]] | + | **[[:Category:Argonaute|Argonaute]] |
| - | *[[:Category:Dicer|Dicer]] | + | **[[:Category:Dicer|Dicer]] |
| - | *[[:Category:Drosha|Drosha]] | + | **[[:Category:Drosha|Drosha]] |
| - | *[[:Category:Piwi|Piwi]] | + | **[[:Category:Piwi|Piwi]] |
| - | *[[:Category:Risc|RISC]] | + | **[[:Category:Risc|RISC]] |
| - | *[[:Category:Rnai|RNAi]] | + | **[[:Category:Rnai|RNAi]] |
| - | *[[:Category:Rnai_suppression|RNAi Suppression]] | + | **[[:Category:Rnai_suppression|RNAi Suppression]] |
| - | *[[:Category:Rna interference|RNA Interference]] | + | **[[:Category:Rna interference|RNA Interference]] |
| - | *[[:Category:Slicer|Slicer]] | + | **[[:Category:Slicer|Slicer]] |
<br /> | <br /> | ||
| - | To view other Proteopedia pages about diseases & drug targets, | + | To view other Proteopedia pages about diseases & drug targets, see: [[Pharmaceutical_Drug_Targets|Pharmaceutical Drug Targets]] |
Current revision
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| guide-strand-containing Argonaute protein silencing complex (3dlh), resolution 3.00Å () | |||||||
|---|---|---|---|---|---|---|---|
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
RNA Interference (RNAi) is a system in living cells that helps control the level of activity of genes. In RNAi, a long double stranded RNA is cleaved into shorter strands. One strand known as the guide strand is complimentary to the mRNA gene product of a gene of interest. This guide strand is incorporated into an RNA-induced silencing complex (RISC) and subsequently binds the mRNA of the gene of interest. After binding, the RNA/RISC complex subsequently silences the mRNA of interest via cleavage of the mRNA strand.
| |||||||
| viral silencing suppressor bound to a siRNA (1rpu), resolution 2.50Å () | |||||||
|---|---|---|---|---|---|---|---|
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Articles in Proteopedia concerning RNA Interference include:
See RNAi in action by viewing this
RNAi Animation.
To view automatically seeded indices concerning RNA Interference, see:
To view other Proteopedia pages about diseases & drug targets, see: Pharmaceutical Drug Targets
