This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1esc
From Proteopedia
(New page: 200px<br /><applet load="1esc" size="450" color="white" frame="true" align="right" spinBox="true" caption="1esc, resolution 2.1Å" /> '''THE MOLECULAR MECHANI...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1esc.gif|left|200px]]<br /><applet load="1esc" size=" | + | [[Image:1esc.gif|left|200px]]<br /><applet load="1esc" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1esc, resolution 2.1Å" /> | caption="1esc, resolution 2.1Å" /> | ||
'''THE MOLECULAR MECHANISM OF ENANTIORECOGNITION BY ESTERASES'''<br /> | '''THE MOLECULAR MECHANISM OF ENANTIORECOGNITION BY ESTERASES'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of a novel esterase from Streptomyces scabies, a | + | The crystal structure of a novel esterase from Streptomyces scabies, a causal agent of the potato scab disease, was solved at 2.1 A resolution. The tertiary fold of the enzyme is substantially different from that of the alpha/beta hydrolase family and unique among all known hydrolases. The active site contains a dyad of Ser 14 and His 283, closely resembling two of the three components of typical Ser-His-Asp(Glu) triads from other serine hydrolases. Proper orientation of the active site imidazol is maintained by a hydrogen bond between the N delta-H group and a main chain oxygen. Thus, the enzyme constitutes the first known natural variation of the chymotrypsin-like triad in which a carboxylic acid is replaced by a neutral hydrogen-bond acceptor. |
==About this Structure== | ==About this Structure== | ||
| - | 1ESC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_scabiei Streptomyces scabiei]. Full crystallographic information is available from [http:// | + | 1ESC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_scabiei Streptomyces scabiei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ESC OCA]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Streptomyces scabiei]] | [[Category: Streptomyces scabiei]] | ||
[[Category: Derewenda, U.]] | [[Category: Derewenda, U.]] | ||
| - | [[Category: Derewenda, Z | + | [[Category: Derewenda, Z S.]] |
[[Category: Patkar, S.]] | [[Category: Patkar, S.]] | ||
| - | [[Category: Schottel, J | + | [[Category: Schottel, J L.]] |
[[Category: Swenson, L.]] | [[Category: Swenson, L.]] | ||
[[Category: Wei, Y.]] | [[Category: Wei, Y.]] | ||
[[Category: hydrolase (serine esterase)]] | [[Category: hydrolase (serine esterase)]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:30:57 2008'' |
Revision as of 10:31, 21 February 2008
|
THE MOLECULAR MECHANISM OF ENANTIORECOGNITION BY ESTERASES
Overview
The crystal structure of a novel esterase from Streptomyces scabies, a causal agent of the potato scab disease, was solved at 2.1 A resolution. The tertiary fold of the enzyme is substantially different from that of the alpha/beta hydrolase family and unique among all known hydrolases. The active site contains a dyad of Ser 14 and His 283, closely resembling two of the three components of typical Ser-His-Asp(Glu) triads from other serine hydrolases. Proper orientation of the active site imidazol is maintained by a hydrogen bond between the N delta-H group and a main chain oxygen. Thus, the enzyme constitutes the first known natural variation of the chymotrypsin-like triad in which a carboxylic acid is replaced by a neutral hydrogen-bond acceptor.
About this Structure
1ESC is a Single protein structure of sequence from Streptomyces scabiei. Full crystallographic information is available from OCA.
Reference
A novel variant of the catalytic triad in the Streptomyces scabies esterase., Wei Y, Schottel JL, Derewenda U, Swenson L, Patkar S, Derewenda ZS, Nat Struct Biol. 1995 Mar;2(3):218-23. PMID:7773790
Page seeded by OCA on Thu Feb 21 12:30:57 2008
