Chaperonin
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1pcq.png|left|200px|thumb|Crystal Structure of Chaperonin, [[1pcq]]]] | [[Image:1pcq.png|left|200px|thumb|Crystal Structure of Chaperonin, [[1pcq]]]] | ||
| - | {{STRUCTURE_1pcq| PDB=1pcq | SIZE=400| SCENE=Chaperonin/Groel_groes_comnplex/1 |right|CAPTION=GroEL/GroES complex, [[1pcq]] }} | + | {{STRUCTURE_1pcq| PDB=1pcq | SIZE=400| SCENE=Chaperonin/Groel_groes_comnplex/1 |right|CAPTION=GroEL/GroES complex with ADP, AlF3, Mg+2 and K+ ions, [[1pcq]] }} |
Revision as of 10:27, 18 August 2013
Chaperonins (CPN) are oligomeric proteins that mediate the folding of polypeptide chains. Group I CPN are found in bacteria, chloroplasts and mitochondria. For an introductory overview, see Chaperonins in Wikipedia.
The most characterized CPN are in the GroEL/GroES complex from Escherichia coli and CPN60/CPN10 from Thermus thermophilus. The larger subunit (GroEL, CPN60) contains 3 domains. The apical domain is the one which binds the substrate. Group II CPNs are found in eukaryotic cytosol and archaea. Thermosome is a CPN complex found in archaea. CCT is a CPN complex found in eukarya.
3D Structures of Chaperonin
Updated on 18-August-2013
Group I
Large subunit
3m6c – MtGroEL1 apical domain – Mycobacterium tuberculosis
1sjp, 3rtk – MtGroEL2 residues 42-539
3fbh, 2eu1, 1j4z, 1kpo, 1oel, 1grl, 2yey – EcGroEL (mutant) - Escherichia coli
3e76, 2nwc, 1xck, 1ss8 – EcGroEL
3c9v, 3cau, 2ynj – EcGroEL – Cryo EM
2c7e, 1gr5, 4aaq - EcGroEL (mutant) – Cryo EM
1dk7 - EcGroEL apical domain
1fy9, 1fya, 1kid, 1jon – EcGroEL apical domain (mutant)
1srv – TtCPN60 apical domain - Thermus thermophilus
3osx - CPN60 apical domain – Xenorhabdus nematophila
1iok – CPN60 – Paracoccus denitrificans
Large subunit binary complex
1kp8 - EcGroEL (mutant) + ATP
4aar, 4aas, 4aau, 4ab2, 4ab3 - EcGroEL (mutant) + ATP – Cryo EM
1sx3 - EcGroEL + ATP
1sx4 - EcGroEL + ADP
4ki8 - EcGroEL (mutant) + ADP
1mnf – EcGroEL + polypeptide
2cgt – EcGroEL + capsid assbly protein GP31 – EM
1dkd - EcGroEL apical domain + polypeptide
Small subunit
3nx6 – GroES residues 40-134 (mutant) – Xanthomonas oryzae
1wnr – TtCPN10 residues 1-94
1p3h, 1hx5 – MtCPN10
1p82, 1p83 - MtCPN10 residues 1-25 - NMR
1egs - GroES residues 19-27 - NMR
Large + small subunit
1svt, 1pcq - EcGroEL + GroES
1pf9, 1aon - EcGroEL + GroES + ADP
2c7c, 2c7d - EcGroEL + GroES – Cryo EM
1gru - EcGroEL + GroES + ATP + ADP – Cryo EM
3zpz, 3zq0, 3zq1 - EcGroEL + GroES + ADP – Cryo EM
1we3 – TtCPN60 + CPN10
1wf4 - TtCPN60 + CPN10 + ADP
Group II
3izh, 3izi, 3izj, 3izk, 3izl, 3izm, 3izn, 3los, 3iyf, 3j3x – MmCPN – Methanococcus maripaludis – EM
3kfb, 3kfe, 3kfk – MmCPN
3j02, 3j03 – MmCPN (mutant) – Cryo EM
3ruq - MmCPN + ADP
3rus - MmCPN (mutant) + ADP
3ruw - MmCPN (mutant) + ADP-AlF3
3ruv - MmCPN (mutant) + ATP analog
1q2v, 1q3r – TkCPN α subunit (mutant) – Thermococcus KS-1
1q3q - TkCPN α subunit (mutant) + AMP-PNP
1q3s - TkCPN α subunit (mutant) + ADP
CCT
2xsm – bCPN CCT – bovine
3p9d, 3p9e, 4d8q, 4d8r – CPN CCT - yeast
3iyg - bCPN CCT – Cryo EM
3ktt - bCPN CCT β subunit – Cryo EM
1gml, 1gn1 - CPN CCT γ subunit apical domain – mouse
Hsp33
3m7m, 1hw7 – EcCPN Hsp33 N-terminal
1xjh - EcCPN Hsp33 C terminal - NMR
1vq0 - CPN Hsp33 – Thermotoga maritima
1vzy - CPN Hsp33 (mutant) – Bacillus subtilis
Thermosome
1a6d - TaTherm α+β subunits – Thermoplasma acidophilum
1a6e - TaTherm α+β subunits + ADP
1ass, 1asx - TaTherm α apical domain
1e0r – TaTherm β apical domain
3ko1 – AtTherm α subunit– Acidianus tengchongensis
3j1b, 3j1c, 3j1e - AtTherm α subunit – Cryo EM
3j1f - AtTherm β subunit + ATP – Cryo EM
3aq1 – Therm – Methanococcoides burtonii
1lep – CPN-10 – Mycobacterium leprae
Proteopedia Page Contributors and Editors (what is this?)
Michal Harel, Alexander Berchansky, Joel L. Sussman, Eric Martz, Jaime Prilusky
