CHEM2052 Tutorial Example4

Chem2052: Example 4 - Renin

The spinning structure you initially view on this page is a protease called Renin. Renin is an aspartyl protease, which cleaves a particular peptide called angiotensinogen.

Background

In later lecture we will look a little more closely at Renin (also known as angiotensinase). This enzyme is involved in a biological pathway leading to elevation of blood pressure, which can be beneficial in many ways. However if this process has become overactive, hypertension (high blood pressure) can result. Hypertension leads to cardiovascular disease which is the leading cause of death globally. The World Health Organisation states "An estimated 17.3 million people died from cardiovascular disease in 2008, representing 30% of all global deaths" see the following web page if you want to know more: Cardiovascular disease facts WHO.

Since the 1970s scientists have been trying to modulate the action of renin by blocking the active site of the enzyme and preventing its function, hence lowering blood pressure. Aliskerin is the only renin inhibitor in clinical use today Renin information Site. However there is still interest in developing new, improved inhibitors. This question looks at a renin inhibitor identified through research at Pfizer.^[1]

Active Site

This representation illustrates the of Renin. Consider the mechanism of the enzymatic cleavage of the natural peptide substrate angiotensinase. Identify the structure of the transition state. In later lectures we will see how knowledge of the transition state can be used to design very effective enzyme inhibitors which mimic this transition state these are (not surprisingly) called "transition state" inhibitors.

Inhibition of Renin

This scene shows the . Which part of the inhibitor binds to the catalytic residues of the active site?

Structural highlights

This is a sample scene created with SAT to by Group, and another to make of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.