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2x4t

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Revision as of 10:49, 25 December 2014

CRYSTAL STRUCTURE OF MHC CLASS I HLA-A2.1 BOUND TO A PEIODATE-CLEAVABLE PEPTIDE

Structural highlights

2x4t is a 6 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
NonStd Res:	,
Related:	1uqs, 1bd2, 2esv, 2ak4, 1ypz, 1im3, 1uxw, 1i7u, 1c16, 1hsa, 1gzp, 2axf, 2bnq, 1w72, 2jcc, 2bck, 1de4, 2vlk, 1exu, 1qrn, 2hla, 1mhe, 1im9, 1eez, 1jht, 1qqd, 1qr1, 1zs8, 1hla, 1jgd, 1i1y, 1vgk, 1age, 1ur7, 1hhg, 1s9x, 1a9e, 1duz, 2clr, 3hla, 1m05, 1tvb, 2v2w, 1onq, 2vlr, 1a1n, 2bvo, 1lp9, 1zsd, 1m6o, 1hhk, 1zt4, 1hsb, 1ce6, 1x7q, 1py4, 1syv, 2j8u, 1sys, 1ogt, 1cg9, 1p7q, 1q94, 1jnj, 1agb, 2d31, 1aqd, 1xz0, 1lds, 1tvh, 1hhh, 1xr8, 2bss, 1a1m, 1e28, 2bvp, 2v2x, 1xr9, 2gj6, 1qlf, 1efx, 2av1, 1tmc, 1qsf, 1jge, 1duy, 1kpr, 2hjl, 1qew, 1w0v, 1k5n, 1ao7, 1xh3, 2bnr, 2bst, 1mi5, 2h26, 1s9y, 1a1o, 2a83, 1agf, 1oga, 2f8o, 2cii, 1i7r, 1jf1, 2c7u, 2f74, 1e27, 1w0w, 1gzq, 1uxs, 1akj, 2hjk, 2vb5, 1agd, 1r3h, 1eey, 1i7t, 1i4f, 1ydp, 2vll, 2bsr, 2vlj, 1b0r, 1b0g, 1of2, 1hhi, 1qse, 2axg, 1a9b, 2bvq, 1agc, 1hhj, 1qvo, 1s9w, 1ktl, 1a6z, 2cik, 2uwe, 1i1f, 2av7, 2x4p, 2x4u, 2x4s, 2x4n, 2x4o, 2x4r, 2x4q
Resources:	FirstGlance, OCA, RCSB, PDBsum

Function

[1A02_HUMAN] Involved in the presentation of foreign antigens to the immune system. [PP65_HCMVA] Counteracts the host antiviral immune response when activated and phosphorylated, by preventing IRF3 from entering the nucleus. Also participates in the transactivation of viral major immediate-early genes by the recruitment of host IFI16 to the promoters pf these genes.^[1] ^[2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Class I major histocompatibility complexes (MHCs) present peptide ligands on the cell surface for recognition by appropriate cytotoxic T cells. The unstable nature of unliganded MHC necessitates the production of recombinant class I complexes through in vitro refolding reactions in the presence of an added excess of peptides. This strategy is not amenable to high-throughput production of vast collections of class I complexes. To address this issue, we recently designed photocaged MHC ligands that can be cleaved by a UV light trigger in the MHC bound state under conditions that do not affect the integrity of the MHC structure. The results obtained with photocaged MHC ligands demonstrate that conditional MHC ligands can form a generally applicable concept for the creation of defined peptide-MHCs. However, the use of UV exposure to mediate ligand exchange is unsuited for a number of applications, due to the lack of UV penetration through cell culture systems and due to the transfer of heat upon UV irradiation, which can induce evaporation. To overcome these limitations, here, we provide proof-of-concept for the generation of defined peptide-MHCs by chemical trigger-induced ligand exchange. The crystal structure of the MHC with the novel chemosensitive ligand showcases that the ligand occupies the expected binding site, in a conformation where the hydroxyl groups should be reactive to periodate. We proceed to validate this technology by producing peptide-MHCs that can be used for T cell detection. The methodology that we describe here should allow loading of MHCs with defined peptides in cell culture devices, thereby permitting antigen-specific T cell expansion and purification for cell therapy. In addition, this technology will be useful to develop miniaturized assay systems for performing high-throughput screens for natural and unnatural MHC ligands.

Class I major histocompatibility complexes loaded by a periodate trigger.,Rodenko B, Toebes M, Celie PH, Perrakis A, Schumacher TN, Ovaa H J Am Chem Soc. 2009 Sep 2;131(34):12305-13. PMID:19655751^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Abate DA, Watanabe S, Mocarski ES. Major human cytomegalovirus structural protein pp65 (ppUL83) prevents interferon response factor 3 activation in the interferon response. J Virol. 2004 Oct;78(20):10995-1006. PMID:15452220 doi:10.1128/JVI.78.20.10995-11006.2004
↑ Cristea IM, Moorman NJ, Terhune SS, Cuevas CD, O'Keefe ES, Rout MP, Chait BT, Shenk T. Human cytomegalovirus pUL83 stimulates activity of the viral immediate-early promoter through its interaction with the cellular IFI16 protein. J Virol. 2010 Aug;84(15):7803-14. doi: 10.1128/JVI.00139-10. Epub 2010 May 26. PMID:20504932 doi:10.1128/JVI.00139-10
↑ Rodenko B, Toebes M, Celie PH, Perrakis A, Schumacher TN, Ovaa H. Class I major histocompatibility complexes loaded by a periodate trigger. J Am Chem Soc. 2009 Sep 2;131(34):12305-13. PMID:19655751 doi:10.1021/ja9037565

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2x4t"

@@ Line 3: / Line 3: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[2x4t]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X4T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2X4T FirstGlance]. <br>
-</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene><br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr>
-<tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PRW:(2R,3R)-4-AMINO-2,3-DIHYDROXYBUTANOIC+ACID'>PRW</scene></td></tr>
+<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PRW:(2R,3R)-4-AMINO-2,3-DIHYDROXYBUTANOIC+ACID'>PRW</scene></td></tr>
-<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1uqs|1uqs]], [[1bd2|1bd2]], [[2esv|2esv]], [[2ak4|2ak4]], [[1ypz|1ypz]], [[1im3|1im3]], [[1uxw|1uxw]], [[1i7u|1i7u]], [[1c16|1c16]], [[1hsa|1hsa]], [[1gzp|1gzp]], [[2axf|2axf]], [[2bnq|2bnq]], [[1w72|1w72]], [[2jcc|2jcc]], [[2bck|2bck]], [[1de4|1de4]], [[2vlk|2vlk]], [[1exu|1exu]], [[1qrn|1qrn]], [[2hla|2hla]], [[1mhe|1mhe]], [[1im9|1im9]], [[1eez|1eez]], [[1jht|1jht]], [[1qqd|1qqd]], [[1qr1|1qr1]], [[1zs8|1zs8]], [[1hla|1hla]], [[1jgd|1jgd]], [[1i1y|1i1y]], [[1vgk|1vgk]], [[1age|1age]], [[1ur7|1ur7]], [[1hhg|1hhg]], [[1s9x|1s9x]], [[1a9e|1a9e]], [[1duz|1duz]], [[2clr|2clr]], [[3hla|3hla]], [[1m05|1m05]], [[1tvb|1tvb]], [[2v2w|2v2w]], [[1onq|1onq]], [[2vlr|2vlr]], [[1a1n|1a1n]], [[2bvo|2bvo]], [[1lp9|1lp9]], [[1zsd|1zsd]], [[1m6o|1m6o]], [[1hhk|1hhk]], [[1zt4|1zt4]], [[1hsb|1hsb]], [[1ce6|1ce6]], [[1x7q|1x7q]], [[1py4|1py4]], [[1syv|1syv]], [[2j8u|2j8u]], [[1sys|1sys]], [[1ogt|1ogt]], [[1cg9|1cg9]], [[1p7q|1p7q]], [[1q94|1q94]], [[1jnj|1jnj]], [[1agb|1agb]], [[2d31|2d31]], [[1aqd|1aqd]], [[1xz0|1xz0]], [[1lds|1lds]], [[1tvh|1tvh]], [[1hhh|1hhh]], [[1xr8|1xr8]], [[2bss|2bss]], [[1a1m|1a1m]], [[1e28|1e28]], [[2bvp|2bvp]], [[2v2x|2v2x]], [[1xr9|1xr9]], [[2gj6|2gj6]], [[1qlf|1qlf]], [[1efx|1efx]], [[2av1|2av1]], [[1tmc|1tmc]], [[1qsf|1qsf]], [[1jge|1jge]], [[1duy|1duy]], [[1kpr|1kpr]], [[2hjl|2hjl]], [[1qew|1qew]], [[1w0v|1w0v]], [[1k5n|1k5n]], [[1ao7|1ao7]], [[1xh3|1xh3]], [[2bnr|2bnr]], [[2bst|2bst]], [[1mi5|1mi5]], [[2h26|2h26]], [[1s9y|1s9y]], [[1a1o|1a1o]], [[2a83|2a83]], [[1agf|1agf]], [[1oga|1oga]], [[2f8o|2f8o]], [[2cii|2cii]], [[1i7r|1i7r]], [[1jf1|1jf1]], [[2c7u|2c7u]], [[2f74|2f74]], [[1e27|1e27]], [[1w0w|1w0w]], [[1gzq|1gzq]], [[1uxs|1uxs]], [[1akj|1akj]], [[2hjk|2hjk]], [[2vb5|2vb5]], [[1agd|1agd]], [[1r3h|1r3h]], [[1eey|1eey]], [[1i7t|1i7t]], [[1i4f|1i4f]], [[1ydp|1ydp]], [[2vll|2vll]], [[2bsr|2bsr]], [[2vlj|2vlj]], [[1b0r|1b0r]], [[1b0g|1b0g]], [[1of2|1of2]], [[1hhi|1hhi]], [[1qse|1qse]], [[2axg|2axg]], [[1a9b|1a9b]], [[2bvq|2bvq]], [[1agc|1agc]], [[1hhj|1hhj]], [[1qvo|1qvo]], [[1s9w|1s9w]], [[1ktl|1ktl]], [[1a6z|1a6z]], [[2cik|2cik]], [[2uwe|2uwe]], [[1i1f|1i1f]], [[2av7|2av7]], [[2x4p|2x4p]], [[2x4u|2x4u]], [[2x4s|2x4s]], [[2x4n|2x4n]], [[2x4o|2x4o]], [[2x4r|2x4r]], [[2x4q|2x4q]]</td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1uqs|1uqs]], [[1bd2|1bd2]], [[2esv|2esv]], [[2ak4|2ak4]], [[1ypz|1ypz]], [[1im3|1im3]], [[1uxw|1uxw]], [[1i7u|1i7u]], [[1c16|1c16]], [[1hsa|1hsa]], [[1gzp|1gzp]], [[2axf|2axf]], [[2bnq|2bnq]], [[1w72|1w72]], [[2jcc|2jcc]], [[2bck|2bck]], [[1de4|1de4]], [[2vlk|2vlk]], [[1exu|1exu]], [[1qrn|1qrn]], [[2hla|2hla]], [[1mhe|1mhe]], [[1im9|1im9]], [[1eez|1eez]], [[1jht|1jht]], [[1qqd|1qqd]], [[1qr1|1qr1]], [[1zs8|1zs8]], [[1hla|1hla]], [[1jgd|1jgd]], [[1i1y|1i1y]], [[1vgk|1vgk]], [[1age|1age]], [[1ur7|1ur7]], [[1hhg|1hhg]], [[1s9x|1s9x]], [[1a9e|1a9e]], [[1duz|1duz]], [[2clr|2clr]], [[3hla|3hla]], [[1m05|1m05]], [[1tvb|1tvb]], [[2v2w|2v2w]], [[1onq|1onq]], [[2vlr|2vlr]], [[1a1n|1a1n]], [[2bvo|2bvo]], [[1lp9|1lp9]], [[1zsd|1zsd]], [[1m6o|1m6o]], [[1hhk|1hhk]], [[1zt4|1zt4]], [[1hsb|1hsb]], [[1ce6|1ce6]], [[1x7q|1x7q]], [[1py4|1py4]], [[1syv|1syv]], [[2j8u|2j8u]], [[1sys|1sys]], [[1ogt|1ogt]], [[1cg9|1cg9]], [[1p7q|1p7q]], [[1q94|1q94]], [[1jnj|1jnj]], [[1agb|1agb]], [[2d31|2d31]], [[1aqd|1aqd]], [[1xz0|1xz0]], [[1lds|1lds]], [[1tvh|1tvh]], [[1hhh|1hhh]], [[1xr8|1xr8]], [[2bss|2bss]], [[1a1m|1a1m]], [[1e28|1e28]], [[2bvp|2bvp]], [[2v2x|2v2x]], [[1xr9|1xr9]], [[2gj6|2gj6]], [[1qlf|1qlf]], [[1efx|1efx]], [[2av1|2av1]], [[1tmc|1tmc]], [[1qsf|1qsf]], [[1jge|1jge]], [[1duy|1duy]], [[1kpr|1kpr]], [[2hjl|2hjl]], [[1qew|1qew]], [[1w0v|1w0v]], [[1k5n|1k5n]], [[1ao7|1ao7]], [[1xh3|1xh3]], [[2bnr|2bnr]], [[2bst|2bst]], [[1mi5|1mi5]], [[2h26|2h26]], [[1s9y|1s9y]], [[1a1o|1a1o]], [[2a83|2a83]], [[1agf|1agf]], [[1oga|1oga]], [[2f8o|2f8o]], [[2cii|2cii]], [[1i7r|1i7r]], [[1jf1|1jf1]], [[2c7u|2c7u]], [[2f74|2f74]], [[1e27|1e27]], [[1w0w|1w0w]], [[1gzq|1gzq]], [[1uxs|1uxs]], [[1akj|1akj]], [[2hjk|2hjk]], [[2vb5|2vb5]], [[1agd|1agd]], [[1r3h|1r3h]], [[1eey|1eey]], [[1i7t|1i7t]], [[1i4f|1i4f]], [[1ydp|1ydp]], [[2vll|2vll]], [[2bsr|2bsr]], [[2vlj|2vlj]], [[1b0r|1b0r]], [[1b0g|1b0g]], [[1of2|1of2]], [[1hhi|1hhi]], [[1qse|1qse]], [[2axg|2axg]], [[1a9b|1a9b]], [[2bvq|2bvq]], [[1agc|1agc]], [[1hhj|1hhj]], [[1qvo|1qvo]], [[1s9w|1s9w]], [[1ktl|1ktl]], [[1a6z|1a6z]], [[2cik|2cik]], [[2uwe|2uwe]], [[1i1f|1i1f]], [[2av7|2av7]], [[2x4p|2x4p]], [[2x4u|2x4u]], [[2x4s|2x4s]], [[2x4n|2x4n]], [[2x4o|2x4o]], [[2x4r|2x4r]], [[2x4q|2x4q]]</td></tr>
-<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2x4t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x4t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2x4t RCSB], [http://www.ebi.ac.uk/pdbsum/2x4t PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2x4t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x4t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2x4t RCSB], [http://www.ebi.ac.uk/pdbsum/2x4t PDBsum]</span></td></tr>
-<table>
+</table>
+== Function ==
+[[http://www.uniprot.org/uniprot/1A02_HUMAN 1A02_HUMAN]] Involved in the presentation of foreign antigens to the immune system. [[http://www.uniprot.org/uniprot/PP65_HCMVA PP65_HCMVA]] Counteracts the host antiviral immune response when activated and phosphorylated, by preventing IRF3 from entering the nucleus. Also participates in the transactivation of viral major immediate-early genes by the recruitment of host IFI16 to the promoters pf these genes.<ref>PMID:15452220</ref> <ref>PMID:20504932</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 35: / Line 37: @@
 </StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Celie, P H.N.]]
+[[Category: Celie, P H.N]]
-[[Category: Ovaa, H.]]
+[[Category: Ovaa, H]]
-[[Category: Perrakis, A.]]
+[[Category: Perrakis, A]]
-[[Category: Rodenko, B.]]
+[[Category: Rodenko, B]]
-[[Category: Schumacher, T N.M.]]
+[[Category: Schumacher, T N.M]]
-[[Category: Toebes, M.]]
+[[Category: Toebes, M]]
 [[Category: Amyloid]]
 [[Category: Amyloidosis]]

2x4t

From Proteopedia

Revision as of 10:49, 25 December 2014

CRYSTAL STRUCTURE OF MHC CLASS I HLA-A2.1 BOUND TO A PEIODATE-CLEAVABLE PEPTIDE

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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