This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
CRISPR-Cas9
From Proteopedia
(Difference between revisions)
| Line 10: | Line 10: | ||
Within Cascade, the <scene name='74/742625/Cv/19'>six Cas7 subunits form a right-handed filament</scene>, with a pitch of ~135Å, around the guide target hybrid. <scene name='74/742625/Cv/16'>Rocket representation of one Cas7 colored by domain: thumb (green), fingers (blue), and palm (purple).</scene> The filament is arranged such that the <scene name='74/742625/Cv/17'>thumb of one Cas7 subunit, composed of an extended β hairpin, extends toward the fingers of the adjacent subunit</scene>. <scene name='74/742625/Cv/18'>90° rotation about axis Z</scene>. | Within Cascade, the <scene name='74/742625/Cv/19'>six Cas7 subunits form a right-handed filament</scene>, with a pitch of ~135Å, around the guide target hybrid. <scene name='74/742625/Cv/16'>Rocket representation of one Cas7 colored by domain: thumb (green), fingers (blue), and palm (purple).</scene> The filament is arranged such that the <scene name='74/742625/Cv/17'>thumb of one Cas7 subunit, composed of an extended β hairpin, extends toward the fingers of the adjacent subunit</scene>. <scene name='74/742625/Cv/18'>90° rotation about axis Z</scene>. | ||
| + | |||
| + | ===Stabilization of the guide-target hybrid by Cas7, Cse1, and Cse2.=== | ||
| + | <scene name='74/742625/Cv/20'>Each 5-bp segment of the hybrid is situated between the palm of one Cas7 subunit (e.g. Cas7.3) and the fingers of the adjacent subunit (e.g. Cas7.4)</scene>. 5-bp segment is colored red. | ||
</StructureSection> | </StructureSection> | ||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 11:33, 22 September 2016
| |||||||||||
References
- ↑ Mulepati S, Heroux A, Bailey S. Crystal structure of a CRISPR RNA-guided surveillance complex bound to a ssDNA target. Science. 2014 Aug 14. pii: 1256996. PMID:25123481 doi:http://dx.doi.org/10.1126/science.1256996
