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==O'Brochta's Sandbox == 0
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==Anopheles arabiensis Insulin Receptor like Protein==
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<StructureSection load='Model.itasser.relax.pdb' size='400' side='right' caption='Anopheles arabiensis InR kinase domain model' scene=''>
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<StructureSection load='Model.itasser.relax.pdb' side='right' caption='Anopheles arabiensis InR kinase domain model' scene='75/750300/Kinase_n_and_c_lobes/1'>
The Anopheles arabiensis Insulin Receptor's kinase domain was modeled by Brian Pierce for the 'An arab ts lethal project'
The Anopheles arabiensis Insulin Receptor's kinase domain was modeled by Brian Pierce for the 'An arab ts lethal project'
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This shows the basic structure of the kinase domain with an <scene name='75/750300/Kinase_n_and_c_lobes/1'>N and C lobe</scene>. The ATP will sit between them just below Beta 1
This shows the basic structure of the kinase domain with an <scene name='75/750300/Kinase_n_and_c_lobes/1'>N and C lobe</scene>. The ATP will sit between them just below Beta 1
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<scene name='75/750300/Inr_atp-cat_loop/1'>This</scene> shows the ATP binding site (purple) and the catalytic loop (green).
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<scene name='75/750300/Inr_atp-cat_loop/3'>This</scene> shows the ATP binding site (purple) and the catalytic loop (green).
Here it is as a <scene name='75/750300/Inr_atp-cat_loopspacefill/1'>spacefilled model</scene>.
Here it is as a <scene name='75/750300/Inr_atp-cat_loopspacefill/1'>spacefilled model</scene>.
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These are <scene name='75/750300/Geneticmutations/1'>three ts mutations</scene> found in other RTKs
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These are <scene name='75/750300/Geneticmutations/1'>three ts mutations</scene> found in other RTKs.
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G1000S (magenta)
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G1000S (magenta), A1133G (orange), T1221I (teal)
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A1133G (orange)
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T1221I (teal)
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And as a <scene name='75/750300/Geneticmutationsspacefilled/1'>spacefilled model</scene>
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This <scene name='75/750300/T1221_spacefilled/1'>threonine</scene> when mutated to isoleucine resulted in ts lethal mutations in the sevenless and EGFR-like genes in D. melanogster
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Using the software TS_pred (a ts mutation prediction program): these are the <scene name='75/750300/Geneticmutations-tspred_rosett/2'>5 best sites</scene> to mutate based on sequence and structure - L1236, I1071, V1196, L1197,I1258 (red)
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Using Rosetta_ts_rbf (another ts mutation prediction program): these are the <scene name='75/750300/Geneticmutations-tspred_rosett/2'>top 5</scene> sites -W1173P, M1075K, Y1208G, L1197P, Y1208Asn (yellow)
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Both TS_pred and Rosetta_ts_rbf had <scene name='75/750300/Tspredrosetta_spacefilled/1'>L1197</scene> in their top 5 (black)
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Most but not all of the actual and predicted mutations are <scene name='75/750300/Tspred_rosett_burried_residues/1'>buried</scene>. Note that T1221 and G1000S are not buried.
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Here are the <scene name='75/750300/Tspred_rosett_hydroph_residues/1'>hydrophobic residues</scene>.
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</StructureSection>

Current revision

Anopheles arabiensis Insulin Receptor like Protein

Anopheles arabiensis InR kinase domain model

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Proteopedia Page Contributors and Editors (what is this?)

David O'Brochta

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