Chaperonin
From Proteopedia
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| - | <StructureSection load='' size='350' side='right' caption='E. coli GroEL ( | + | <StructureSection load='' size='350' side='right' caption='E. coli GroEL (green)/GroES (magenta) complex with ADP, AlF3, Mg+2 and K+ ions (PDB entry [[1pcq]])' scene='44/445432/Cv/1'> |
[[Image:1pcq.png|left|200px|thumb|Crystal Structure of Chaperonin, [[1pcq]]]] | [[Image:1pcq.png|left|200px|thumb|Crystal Structure of Chaperonin, [[1pcq]]]] | ||
'''Chaperonins''' (CPN) are oligomeric proteins that mediate the folding of polypeptide chains. '''Group I CPN''' are found in bacteria, chloroplasts and mitochondria. For an introductory overview, see [http://en.wikipedia.org/wiki/Chaperonins Chaperonins in Wikipedia]. | '''Chaperonins''' (CPN) are oligomeric proteins that mediate the folding of polypeptide chains. '''Group I CPN''' are found in bacteria, chloroplasts and mitochondria. For an introductory overview, see [http://en.wikipedia.org/wiki/Chaperonins Chaperonins in Wikipedia]. | ||
The most characterized CPN are in the GroEL/GroES complex from ''Escherichia coli'' and CPN60/CPN10 from ''Thermus thermophilus''.<ref>PMID:18987317</ref> The larger subunit (GroEL, CPN60) contains 3 domains: apical, intermediate and equatorial domain. The apical domain is the one which binds the polypeptide substrate. The equatorial domains binds the nucleotide. '''Group II CPNs''' are found in eukaryotic cytosol and archaea. '''Thermosome''' is a CPN complex found in archaea.<ref>PMID:9546398</ref> '''CCT''' is a CPN complex found in eukarya.<ref>PMID:22503819</ref> | The most characterized CPN are in the GroEL/GroES complex from ''Escherichia coli'' and CPN60/CPN10 from ''Thermus thermophilus''.<ref>PMID:18987317</ref> The larger subunit (GroEL, CPN60) contains 3 domains: apical, intermediate and equatorial domain. The apical domain is the one which binds the polypeptide substrate. The equatorial domains binds the nucleotide. '''Group II CPNs''' are found in eukaryotic cytosol and archaea. '''Thermosome''' is a CPN complex found in archaea.<ref>PMID:9546398</ref> '''CCT''' is a CPN complex found in eukarya.<ref>PMID:22503819</ref> | ||
| - | *<scene name='44/445432/Cv/ | + | *<scene name='44/445432/Cv/2'>E. coli GroEL/GroES complex</scene> (GroEL in green, GroES in magenta, PDB entry [[1pcq]]). |
| - | *<scene name='44/445432/Cv/ | + | *<scene name='44/445432/Cv/3'>GroEL/GroES complex with ADP, AlF3, Mg+2 and K+ ions</scene>. |
| - | *<scene name='44/445432/Cv/5'>K+ ion coordination site</scene>. | + | *<scene name='44/445432/Cv/5'>K+ ion coordination site</scene>. GroEL in cyan. |
*<scene name='44/445432/Cv/6'>AlF3 binding site</scene>. | *<scene name='44/445432/Cv/6'>AlF3 binding site</scene>. | ||
*<scene name='44/445432/Cv/7'>Mg+2 ion coordination site</scene>. | *<scene name='44/445432/Cv/7'>Mg+2 ion coordination site</scene>. | ||
Revision as of 14:57, 9 July 2018
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3D Structures of Chaperonin
Updated on 09-July-2018
References
- ↑ Apetri AC, Horwich AL. Chaperonin chamber accelerates protein folding through passive action of preventing aggregation. Proc Natl Acad Sci U S A. 2008 Nov 11;105(45):17351-5. doi:, 10.1073/pnas.0809794105. Epub 2008 Nov 5. PMID:18987317 doi:http://dx.doi.org/10.1073/pnas.0809794105
- ↑ Ditzel L, Lowe J, Stock D, Stetter KO, Huber H, Huber R, Steinbacher S. Crystal structure of the thermosome, the archaeal chaperonin and homolog of CCT. Cell. 1998 Apr 3;93(1):125-38. PMID:9546398
- ↑ Leitner A, Joachimiak LA, Bracher A, Monkemeyer L, Walzthoeni T, Chen B, Pechmann S, Holmes S, Cong Y, Ma B, Ludtke S, Chiu W, Hartl FU, Aebersold R, Frydman J. The Molecular Architecture of the Eukaryotic Chaperonin TRiC/CCT. Structure. 2012 May 9;20(5):814-25. Epub 2012 Apr 12. PMID:22503819 doi:10.1016/j.str.2012.03.007
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