Johnson's Monday Lab Sandbox for Insulin Receptor
From Proteopedia
(Difference between revisions)
| Line 13: | Line 13: | ||
===How Insulin Binds=== | ===How Insulin Binds=== | ||
| - | The insulin receptor unit has four separate sites for the insulin molecule to bind to. There are two pairs of two identical binding sites referred to as 1 and 1' and then 2 and 2'. The insulin molecules bind to these sites mostly through [http://en.wikipedia.org/wiki/Hydrophobic_effect hydrophobic interactions]. Despite a majority of the interactions being similar, sites 1 and 1' have a higher binding affinity than sites 2 and 2' due to site one having a larger surface area (706 square angstroms) exposed for insulin to bind to compared to site 2 (394 square angstroms)<ref name="Uchikawa"> | + | The insulin receptor unit has four separate sites for the insulin molecule to bind to. There are two pairs of two identical binding sites referred to as 1 and 1' and then 2 and 2'. The insulin molecules bind to these sites mostly through [http://en.wikipedia.org/wiki/Hydrophobic_effect hydrophobic interactions]. Despite a majority of the interactions being similar, sites 1 and 1' have a higher binding affinity than sites 2 and 2' due to site one having a larger surface area (706 square angstroms) exposed for insulin to bind to compared to site 2 (394 square angstroms)<ref name="Uchikawa">. |
It was found that at least three insulin molecules would have to bound to the receptor for the receptor to take on its active “T-state” conformation <ref name=”Uchikawa”>. The difference between have the fully bound state with four insulins and the three insulin bound state is minimal compared to the difference between two and three insulins bound <ref name=”Uchikawa”. | It was found that at least three insulin molecules would have to bound to the receptor for the receptor to take on its active “T-state” conformation <ref name=”Uchikawa”>. The difference between have the fully bound state with four insulins and the three insulin bound state is minimal compared to the difference between two and three insulins bound <ref name=”Uchikawa”. | ||
Revision as of 22:57, 21 March 2020
Your Heading Here (maybe something like 'Structure')
| |||||||||||
References
- ↑ Hanson, R. M., Prilusky, J., Renjian, Z., Nakane, T. and Sussman, J. L. (2013), JSmol and the Next-Generation Web-Based Representation of 3D Molecular Structure as Applied to Proteopedia. Isr. J. Chem., 53:207-216. doi:http://dx.doi.org/10.1002/ijch.201300024
- ↑ Herraez A. Biomolecules in the computer: Jmol to the rescue. Biochem Mol Biol Educ. 2006 Jul;34(4):255-61. doi: 10.1002/bmb.2006.494034042644. PMID:21638687 doi:10.1002/bmb.2006.494034042644
- ↑ 3.0 3.1 . It was found that at least three insulin molecules would have to bound to the receptor for the receptor to take on its active “T-state” conformation <ref>. The difference between have the fully bound state with four insulins and the three insulin bound state is minimal compared to the difference between two and three insulins bound <ref name=”Uchikawa”. [http://</li> <li id="cite_note-Boucher-3">[[#cite_ref-Boucher_3-0|↑]] Boucher J, Kleinridders A, Kahn CR. Insulin receptor signaling in normal and insulin-resistant states. Cold Spring Harb Perspect Biol. 2014 Jan 1;6(1). pii: 6/1/a009191. doi:, 10.1101/cshperspect.a009191. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/24384568 24384568] doi:[http://dx.doi.org/10.1101/cshperspect.a009191 http://dx.doi.org/10.1101/cshperspect.a009191]</li> <li id="cite_note-Meyts-4">[[#cite_ref-Meyts_4-0|↑]] De Meyts P. The structural basis of insulin and insulin-like growth factor-I receptor binding and negative co-operativity, and its relevance to mitogenic versus metabolic signalling. Diabetologia. 1994 Sep;37 Suppl 2:S135-48. doi: 10.1007/bf00400837. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7821729 7821729] doi:[http://dx.doi.org/10.1007/bf00400837 http://dx.doi.org/10.1007/bf00400837]</li></ol></ref>
Student Contributors
- Maxwell Todd
- Abby Hillan
- Andrew Scheel
