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3ibg

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{{Seed}}
 
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[[Image:3ibg.png|left|200px]]
 
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<!--
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==Crystal structure of Aspergillus fumigatus Get3 with bound ADP==
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The line below this paragraph, containing "STRUCTURE_3ibg", creates the "Structure Box" on the page.
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<StructureSection load='3ibg' size='340' side='right'caption='[[3ibg]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3ibg]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IBG OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3IBG FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
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-->
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arsenite-transporting_ATPase Arsenite-transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.16 3.6.3.16] </span></td></tr>
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{{STRUCTURE_3ibg| PDB=3ibg | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3ibg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ibg OCA], [http://pdbe.org/3ibg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ibg RCSB], [http://www.ebi.ac.uk/pdbsum/3ibg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ibg ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[http://www.uniprot.org/uniprot/GET3_ASPFU GET3_ASPFU]] ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting (By similarity).[HAMAP-Rule:MF_03112]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ib/3ibg_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ibg ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The Get3 ATPase directs the delivery of tail-anchored (TA) proteins to the endoplasmic reticulum (ER). TA-proteins are characterized by having a single transmembrane helix (TM) at their extreme C terminus and include many essential proteins, such as SNAREs, apoptosis factors, and protein translocation components. These proteins cannot follow the SRP-dependent co-translational pathway that typifies most integral membrane proteins; instead, post-translationally, these proteins are recognized and bound by Get3 then delivered to the ER in the ATP dependent Get pathway. To elucidate a molecular mechanism for TA protein binding by Get3 we have determined three crystal structures in apo and ADP forms from Saccharomyces cerevisae (ScGet3-apo) and Aspergillus fumigatus (AfGet3-apo and AfGet3-ADP). Using structural information, we generated mutants to confirm important interfaces and essential residues. These results point to a model of how Get3 couples ATP hydrolysis to the binding and release of TA-proteins.
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===Crystal structure of Aspergillus fumigatus Get3 with bound ADP===
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Model for eukaryotic tail-anchored protein binding based on the structure of Get3.,Suloway CJ, Chartron JW, Zaslaver M, Clemons WM Jr Proc Natl Acad Sci U S A. 2009 Sep 1;106(35):14849-54. Epub 2009 Aug 14. PMID:19706470<ref>PMID:19706470</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3ibg" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_19706470}}, adds the Publication Abstract to the page
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*[[ATPase 3D structures|ATPase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 19706470 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19706470}}
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__TOC__
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</StructureSection>
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==About this Structure==
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3IBG is a 6 chains structure of sequences from [http://en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IBG OCA].
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==Reference==
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<ref group="xtra">PMID:19706470</ref><references group="xtra"/>
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[[Category: Arsenite-transporting ATPase]]
[[Category: Arsenite-transporting ATPase]]
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[[Category: Aspergillus fumigatus]]
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[[Category: Large Structures]]
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[[Category: Chartron, J W.]]
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[[Category: Chartron, J W]]
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[[Category: Jr., W M.Clemons.]]
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[[Category: Clemons, W M]]
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[[Category: Suloway, C J.M.]]
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[[Category: Suloway, C J.M]]
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[[Category: Zaslaver, M.]]
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[[Category: Zaslaver, M]]
[[Category: Deviant walker a motif]]
[[Category: Deviant walker a motif]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: Nucleotide binding]]
[[Category: Nucleotide binding]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 21 09:33:45 2009''
 

Current revision

Crystal structure of Aspergillus fumigatus Get3 with bound ADP

PDB ID 3ibg

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