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Proteasome

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[[Image:3gpt.jpg|left|200px|thumb|Crystal Structure of Proteasome, [[3gpt]]]]
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<StructureSection load='' size='350' side='right' scene='41/417543/Cv/1' caption='Yeast proteasome 20S core complex with salinosporamide derivative, [[3gpt]]'>
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{{STRUCTURE_3gpt| PDB=3gpt | SIZE=300| SCENE= |right|CAPTION=Proteasome, [[3gpt]] }}
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== Function ==
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[[Proteasome]] (PRTS) are large protein complexes which degrade unneeded proteins into small polypeptides<ref>PMID:22350895</ref>. The '''26S PRTS''' is composed of a central '''20S core particle''' which contains 4 stacked rings each with several members and two 19S caps. The 19S cap is composed of a base with 10 proteins six of which are ATPases and a lid which contains 9 proteins which bind polyubiquitin. <br />
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For more details see [[3unb]].
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== Structural highlights ==
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[[Proteasome]] (PRTS) are large protein complexes which degrade unneeded proteins into small polypeptides. The 26S PRTS is composed of a central 20S core particle which contains 4 stacked rings each with several members and two 19S caps. The core particle two outer rings contain 7 alpha subunits which form the PRTS gate. The two inner rings contain 6 beta subunits with protease activity. The 19S cap is composed of a base with 10 proteins six of which are ATPases and a lid which contains 9 proteins which binds polyubiquitin. The images at the left and at the right correspond to one representative Proteasome, ''i.e.'' the crystal structure of yeast Proteasome ([[3gpt]]).
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The core particle two outer rings contain 7 α subunits (Y7, Y13, PRE6, PRE5, PUP2, C1, C7-α) which form the PRTS gate. The two inner rings contain 7 β subunits (PRE2, PRE4, PRE3, PUP1, PUP3, C5, C11) with protease activity<ref>PMID:8811196</ref>.
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*<scene name='41/417543/Cv/2'>α subunits and β subunits</scene>.
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{{TOC limit|limit=2}}
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*<scene name='41/417543/Cv/6'>Salinosporamide derivative binding site</scene> of chain H.
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*<scene name='41/417543/Cv/7'>Covalent bond between Salinosporamide derivative and Thr1</scene>.
== 3D Structures of Proteasome ==
== 3D Structures of Proteasome ==
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[[Proteasome 3D structures]]
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== See Also ==
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[[PROTAC]] (Proteolysis-Targeting Chimera), an alternative to conventional enzyme inhibitors, by enabling proteasome induced degradation of the target protein.
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</StructureSection>
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== References ==
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=== 20S particle ===
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<references/>
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[[Category:Topic Page]]
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[[3mfe]], [[3mi0]], [[2fhg]] – Mt20S core – ''Mycobacterium tuberculosis''<br />
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[[3gpt]], [[3gpw]], [[1g0u]] – y20S core - yeast<br />
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[[1ryp]] - y20S core (mutant)<br />
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[[3mka]], [[3hfa]] – Mt20S core (mutant)<br />
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[[3c91]], [[3c92]], [[1pma]] – Ta20S core - ''Themoplasma acidophilum''<br />
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[[3h4p]] – 20S core – ''Methanocaldococcus janaschii''<br />
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[[2h6j]], [[1q5r]] – Re20S core (mutant) – ''Rhodococcus erythropolis''<br />
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[[1q5q]] - Re20S core<br />
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[[1j2q]] – Af20S core - ''Archaeoglobus fulgidus''<br />
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[[1iru]] – 20S core - bovine<br />
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=== 20S+activator protein ===
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[[1vsy]], [[1l5q]] – y20S core+ Blm10 <br />
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[[1z7q]], [[1fnt]] – y20S core+PA26<br />
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[[3ipm]], [[3jrm]], [[3jse]], [[3jtl]], [[1ya7]], [[1yar]], [[1yau]] - Taa-PRTS chains A-G+ Tab-PRTS chains H-N+PA26 chains O-U <br />
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=== 20S+inhibitor ===
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[[3hye]], [[3gpj]], [[3dy3]], [[3dy4]], [[3e47]], [[3d29]], [[3bdm]], [[2gpl]], [[2fak]], [[2f16]], [[1jd2]], [[1g65]] – y20S core+inhibitor<br />
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[[3h6f]], [[3h6i]], [[3hf9]], [[2fhh]] – Mt20S core+inhibitor<br />
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=== Partial 20S ===
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[[2ku1]], [[2ku2]] – Taa-PRTS chains A-G <br />
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[[3fp9]] – MtATPase chains A-L<br />
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[[1j2p]] – Af α subunit<br />
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Current revision

Yeast proteasome 20S core complex with salinosporamide derivative, 3gpt

Drag the structure with the mouse to rotate

References

  1. ↑ Saeki Y, Tanaka K. Assembly and function of the proteasome. Methods Mol Biol. 2012;832:315-37. doi: 10.1007/978-1-61779-474-2_22. PMID:22350895 doi:http://dx.doi.org/10.1007/978-1-61779-474-2_22
  2. ↑ Coux O, Tanaka K, Goldberg AL. Structure and functions of the 20S and 26S proteasomes. Annu Rev Biochem. 1996;65:801-47. PMID:8811196 doi:http://dx.doi.org/10.1146/annurev.bi.65.070196.004101

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Jaime Prilusky, Joel L. Sussman

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