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| | ==Crystal structure of the Sgt2 N domain and the Get5 UBL domain complex== | | ==Crystal structure of the Sgt2 N domain and the Get5 UBL domain complex== |
| - | <StructureSection load='3zdm' size='340' side='right' caption='[[3zdm]], [[Resolution|resolution]] 1.80Å' scene=''> | + | <StructureSection load='3zdm' size='340' side='right'caption='[[3zdm]], [[Resolution|resolution]] 1.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3zdm]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZDM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZDM FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3zdm]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZDM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZDM FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zdm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zdm OCA], [http://pdbe.org/3zdm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3zdm RCSB], [http://www.ebi.ac.uk/pdbsum/3zdm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3zdm ProSAT]</span></td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zdm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zdm OCA], [https://pdbe.org/3zdm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zdm RCSB], [https://www.ebi.ac.uk/pdbsum/3zdm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zdm ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SGT2_YEAST SGT2_YEAST]] Co-chaperone that binds to the molecular chaperone Hsp70 (SSA1 and SSA2). Regulates Hsp70 ATPase activity (By similarity). Required for recovery from heat shock.<ref>PMID:12482202</ref> [[http://www.uniprot.org/uniprot/MDY2_YEAST MDY2_YEAST]] Required for efficient mating. Involved in the production of alpha-factor, the KAR9 and TUB1 location to the shmoo tip and nuclear migration into pheromone-induced shmoos.<ref>PMID:10514570</ref> <ref>PMID:16390866</ref> | + | [[https://www.uniprot.org/uniprot/SGT2_YEAST SGT2_YEAST]] Co-chaperone that binds to the molecular chaperone Hsp70 (SSA1 and SSA2). Regulates Hsp70 ATPase activity (By similarity). Required for recovery from heat shock.<ref>PMID:12482202</ref> [[https://www.uniprot.org/uniprot/MDY2_YEAST MDY2_YEAST]] Required for efficient mating. Involved in the production of alpha-factor, the KAR9 and TUB1 location to the shmoo tip and nuclear migration into pheromone-induced shmoos.<ref>PMID:10514570</ref> <ref>PMID:16390866</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 3zdm" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 3zdm" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Small glutamine-rich tetratricopeptide repeat containing protein alpha|Small glutamine-rich tetratricopeptide repeat containing protein alpha]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Atcc 18824]] | | [[Category: Atcc 18824]] |
| | + | [[Category: Large Structures]] |
| | [[Category: Hsiao, C D]] | | [[Category: Hsiao, C D]] |
| | [[Category: Li, Y C]] | | [[Category: Li, Y C]] |
| | [[Category: Tung, J Y]] | | [[Category: Tung, J Y]] |
| | [[Category: Chaperone-signaling protein complex]] | | [[Category: Chaperone-signaling protein complex]] |
| Structural highlights
Function
[SGT2_YEAST] Co-chaperone that binds to the molecular chaperone Hsp70 (SSA1 and SSA2). Regulates Hsp70 ATPase activity (By similarity). Required for recovery from heat shock.[1] [MDY2_YEAST] Required for efficient mating. Involved in the production of alpha-factor, the KAR9 and TUB1 location to the shmoo tip and nuclear migration into pheromone-induced shmoos.[2] [3]
Publication Abstract from PubMed
The insertion of tail-anchored membrane (TA) proteins into the appropriate membrane is a post-translational event that requires stabilization of the transmembrane domain and targeting to the proper destination. Sgt2, a small glutamine-rich tetratricopeptide-repeat protein, is a heat-shock protein cognate (HSC) co-chaperone that preferentially binds endoplasmic reticulum-destined TA proteins and directs them to the GET pathway via Get4 and Get5. The N-terminal domain of Sgt2 seems to exert dual functions. It mediates Get5 interaction and allows substrate delivery to Get3. Following the N-terminus of Get5 is a ubiquitin-like (Ubl) domain that interacts with the N-terminus of Sgt2. Here, the crystal structure of the Sgt2 dimerization domain complexed with the Get5 Ubl domain (Sgt2N-Get5Ubl) is reported. This complex reveals an intimate interaction between one Sgt2 dimer and one Get5 monomer. This research further demonstrates that hydrophobic residues from both Sgt2 and Get5 play an important role in cell survival under heat stress. This study provides detailed molecular insights into the specific binding of this GET-pathway complex.
Structure of the Sgt2 dimerization domain complexed with the Get5 UBL domain involved in the targeting of tail-anchored membrane proteins to the endoplasmic reticulum.,Tung JY, Li YC, Lin TW, Hsiao CD Acta Crystallogr D Biol Crystallogr. 2013 Oct 1;69(Pt 10):2081-2090. Epub 2013, Sep 20. PMID:24100326[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Angeletti PC, Walker D, Panganiban AT. Small glutamine-rich protein/viral protein U-binding protein is a novel cochaperone that affects heat shock protein 70 activity. Cell Stress Chaperones. 2002 Jul;7(3):258-68. PMID:12482202
- ↑ Iwanejko L, Smith KN, Loeillet S, Nicolas A, Fabre F. Disruption and functional analysis of six ORFs on chromosome XV: YOL117w, YOL115w ( TRF4), YOL114c, YOL112w ( MSB4), YOL111c and YOL072w. Yeast. 1999 Oct;15(14):1529-39. PMID:10514570 doi:<1529::AID-YEA457>3.0.CO;2-Y 10.1002/(SICI)1097-0061(199910)15:14<1529::AID-YEA457>3.0.CO;2-Y
- ↑ Hu Z, Potthoff B, Hollenberg CP, Ramezani-Rad M. Mdy2, a ubiquitin-like (UBL)-domain protein, is required for efficient mating in Saccharomyces cerevisiae. J Cell Sci. 2006 Jan 15;119(Pt 2):326-38. Epub 2006 Jan 3. PMID:16390866 doi:jcs.02754
- ↑ Tung JY, Li YC, Lin TW, Hsiao CD. Structure of the Sgt2 dimerization domain complexed with the Get5 UBL domain involved in the targeting of tail-anchored membrane proteins to the endoplasmic reticulum. Acta Crystallogr D Biol Crystallogr. 2013 Oct 1;69(Pt 10):2081-2090. Epub 2013, Sep 20. PMID:24100326 doi:http://dx.doi.org/10.1107/S0907444913019379
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