This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


5vhh

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: '''Unreleased structure''' The entry 5vhh is ON HOLD Authors: Lu, Y., Wu, J., Dong, Y., Chen, S., Sun, S., Ma, Y.B., Ouyang, Q., Finley, D., Kirschner, M.W., Mao, Y. Description: Confo...)
Current revision (10:07, 16 August 2023) (edit) (undo)
 
(6 intermediate revisions not shown.)
Line 1: Line 1:
-
'''Unreleased structure'''
 
-
The entry 5vhh is ON HOLD
+
==Conformational Landscape of the p28-Bound Human Proteasome Regulatory Particle==
 +
<SX load='5vhh' size='340' side='right' viewer='molstar' caption='[[5vhh]], [[Resolution|resolution]] 6.10&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[5vhh]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VHH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5VHH FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 6.1&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5vhh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vhh OCA], [https://pdbe.org/5vhh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5vhh RCSB], [https://www.ebi.ac.uk/pdbsum/5vhh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5vhh ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/PSMD3_HUMAN PSMD3_HUMAN] Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The proteasome holoenzyme is activated by its regulatory particle (RP) consisting of two subcomplexes, the lid and the base. A key event in base assembly is the formation of a heterohexameric ring of AAA-ATPases, which is guided by at least four RP assembly chaperones in mammals: PAAF1, p28/gankyrin, p27/PSMD9, and S5b. Using cryogenic electron microscopy, we analyzed the non-AAA structure of the p28-bound human RP at 4.5 A resolution and determined seven distinct conformations of the Rpn1-p28-AAA subcomplex within the p28-bound RP at subnanometer resolutions. Remarkably, the p28-bound AAA ring does not form a channel in the free RP and spontaneously samples multiple "open" and "closed" topologies at the Rpt2-Rpt6 and Rpt3-Rpt4 interfaces. Our analysis suggests that p28 assists the proteolytic core particle to select a specific conformation of the ATPase ring for RP engagement and is released in a shoehorn-like fashion in the last step of the chaperone-mediated proteasome assembly.
-
Authors: Lu, Y., Wu, J., Dong, Y., Chen, S., Sun, S., Ma, Y.B., Ouyang, Q., Finley, D., Kirschner, M.W., Mao, Y.
+
Conformational Landscape of the p28-Bound Human Proteasome Regulatory Particle.,Lu Y, Wu J, Dong Y, Chen S, Sun S, Ma YB, Ouyang Q, Finley D, Kirschner MW, Mao Y Mol Cell. 2017 Jul 20;67(2):322-333.e6. doi: 10.1016/j.molcel.2017.06.007. Epub, 2017 Jul 6. PMID:28689658<ref>PMID:28689658</ref>
-
Description: Conformational Landscape of the p28-Bound Human Proteasome Regulatory Particle
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
[[Category: Unreleased Structures]]
+
</div>
-
[[Category: Ma, Y.B]]
+
<div class="pdbe-citations 5vhh" style="background-color:#fffaf0;"></div>
-
[[Category: Kirschner, M.W]]
+
 
-
[[Category: Lu, Y]]
+
==See Also==
-
[[Category: Ouyang, Q]]
+
*[[Ankyrin 3D structures|Ankyrin 3D structures]]
-
[[Category: Wu, J]]
+
*[[Proteasome 3D structures|Proteasome 3D structures]]
-
[[Category: Mao, Y]]
+
== References ==
-
[[Category: Chen, S]]
+
<references/>
-
[[Category: Sun, S]]
+
__TOC__
-
[[Category: Dong, Y]]
+
</SX>
-
[[Category: Finley, D]]
+
[[Category: Homo sapiens]]
 +
[[Category: Large Structures]]
 +
[[Category: Chen S]]
 +
[[Category: Dong Y]]
 +
[[Category: Finley D]]
 +
[[Category: Kirschner MW]]
 +
[[Category: Lu Y]]
 +
[[Category: Ma YB]]
 +
[[Category: Mao Y]]
 +
[[Category: Ouyang Q]]
 +
[[Category: Sun S]]
 +
[[Category: Wu J]]

Current revision

Conformational Landscape of the p28-Bound Human Proteasome Regulatory Particle

5vhh, resolution 6.10Å

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5vhh"
Personal tools