5lyp
From Proteopedia
(Difference between revisions)
| (One intermediate revision not shown.) | |||
| Line 1: | Line 1: | ||
==Crystal structure of the Tpr Domain of Sgt2.== | ==Crystal structure of the Tpr Domain of Sgt2.== | ||
| - | <StructureSection load='5lyp' size='340' side='right' caption='[[5lyp]], [[Resolution|resolution]] 1.55Å' scene=''> | + | <StructureSection load='5lyp' size='340' side='right'caption='[[5lyp]], [[Resolution|resolution]] 1.55Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[5lyp]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LYP OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[5lyp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LYP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LYP FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BO4:BORATE+ION'>BO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BO4:BORATE+ION'>BO4</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lyp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lyp OCA], [https://pdbe.org/5lyp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lyp RCSB], [https://www.ebi.ac.uk/pdbsum/5lyp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lyp ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
| - | [ | + | [https://www.uniprot.org/uniprot/SGT2_YEAST SGT2_YEAST] Co-chaperone that binds to the molecular chaperone Hsp70 (SSA1 and SSA2). Regulates Hsp70 ATPase activity (By similarity). Required for recovery from heat shock.<ref>PMID:12482202</ref> |
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Small glutamine-rich tetratricopeptide repeat-containing protein 2 (Sgt2) is a multi-module co-chaperone involved in several protein quality control pathways. The TPR domain of Sgt2 and several other proteins, including SGTA, Hop, and CHIP, is a highly conserved motif known to form transient complexes with molecular chaperones such as Hsp70 and Hsp90. In this work, we present the first high resolution crystal structures of Sgt2_TPR alone and in complex with a C-terminal peptide PTVEEVD from heat shock protein, Ssa1. Using nuclear magnetic resonance spectroscopy and isothermal titration calorimetry, we demonstrate that Sgt2_TPR interacts with peptides corresponding to the C-termini of Ssa1, Hsc82, and Ybr137wp with similar binding modes and affinities. | ||
| + | |||
| + | Structure and Interactions of the TPR Domain of Sgt2 with Yeast Chaperones and Ybr137wp.,Krysztofinska EM, Evans NJ, Thapaliya A, Murray JW, Morgan RML, Martinez-Lumbreras S, Isaacson RL Front Mol Biosci. 2017 Oct 11;4:68. doi: 10.3389/fmolb.2017.00068. eCollection, 2017. PMID:29075633<ref>PMID:29075633</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5lyp" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Small glutamine-rich tetratricopeptide repeat containing protein alpha|Small glutamine-rich tetratricopeptide repeat containing protein alpha]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: Evans | + | [[Category: Large Structures]] |
| - | [[Category: Isaacson | + | [[Category: Saccharomyces cerevisiae]] |
| - | [[Category: Krysztofinska | + | [[Category: Evans NJ]] |
| - | [[Category: Martinez-Lumbreras | + | [[Category: Isaacson RL]] |
| - | [[Category: Morgan | + | [[Category: Krysztofinska EM]] |
| - | [[Category: Murray | + | [[Category: Martinez-Lumbreras S]] |
| - | [[Category: Thapaliya | + | [[Category: Morgan RML]] |
| - | + | [[Category: Murray JW]] | |
| + | [[Category: Thapaliya A]] | ||
Current revision
Crystal structure of the Tpr Domain of Sgt2.
| |||||||||||
