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6j43
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==Proteinase K determined by PAL-XFEL== | ==Proteinase K determined by PAL-XFEL== | ||
| - | <StructureSection load='6j43' size='340' side='right'caption='[[6j43]]' scene=''> | + | <StructureSection load='6j43' size='340' side='right'caption='[[6j43]], [[Resolution|resolution]] 1.85Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J43 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6J43 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6j43]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Parengyodontium_album Parengyodontium album]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J43 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6J43 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6j43 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j43 OCA], [https://pdbe.org/6j43 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6j43 RCSB], [https://www.ebi.ac.uk/pdbsum/6j43 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6j43 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6j43 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j43 OCA], [https://pdbe.org/6j43 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6j43 RCSB], [https://www.ebi.ac.uk/pdbsum/6j43 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6j43 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/PRTK_PARAQ PRTK_PARAQ] Hydrolyzes keratin at aromatic and hydrophobic residues. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Microcrystal delivery methods are pivotal in the use of serial femtosecond crystallography (SFX) to resolve the macromolecular structures of proteins. Here, the development of a novel technique and instruments for efficiently delivering microcrystals for SFX are presented. The new method, which relies on a one-dimensional fixed-target system that includes a microcrystal container, consumes an extremely low amount of sample compared with conventional two-dimensional fixed-target techniques at ambient temperature. This novel system can deliver soluble microcrystals without highly viscous carrier media and, moreover, can be used as a microcrystal growth device for SFX. Diffraction data collection utilizing this advanced technique along with a real-time visual servo scan system has been successfully demonstrated for the structure determination of proteinase K microcrystals at 1.85 A resolution. | ||
| + | |||
| + | Application of a high-throughput microcrystal delivery system to serial femtosecond crystallography.,Lee D, Park S, Lee K, Kim J, Park G, Nam KH, Baek S, Chung WK, Lee JL, Cho Y, Park J J Appl Crystallogr. 2020 Mar 25;53(Pt 2):477-485. doi: 10.1107/S1600576720002423., eCollection 2020 Apr 1. PMID:32280322<ref>PMID:32280322</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6j43" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Proteinase 3D structures|Proteinase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Parengyodontium album]] | ||
[[Category: Lee SJ]] | [[Category: Lee SJ]] | ||
[[Category: Park J]] | [[Category: Park J]] | ||
Current revision
Proteinase K determined by PAL-XFEL
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