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4adl
From Proteopedia
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| - | [[Image:4adl.png|left|200px]] | ||
| - | + | ==Crystal structures of Rv1098c in complex with malate== | |
| + | <StructureSection load='4adl' size='340' side='right'caption='[[4adl]], [[Resolution|resolution]] 2.20Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4adl]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ADL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ADL FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LMR:(2S)-2-HYDROXYBUTANEDIOIC+ACID'>LMR</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4adl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4adl OCA], [https://pdbe.org/4adl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4adl RCSB], [https://www.ebi.ac.uk/pdbsum/4adl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4adl ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/FUMC_MYCTU FUMC_MYCTU] Catalyzes the reversible addition of water to fumarate to give L-malate. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | rv1098c, an essential gene in Mycobacterium tuberculosis, codes for a class II fumarase. We describe here the crystal structure of Rv1098c in complex with l-malate, fumarate or the competitive inhibitor meso-tartrate. The models reveal that substrate binding promotes the closure of the active site through conformational changes involving the catalytic SS-loop and the C-terminal domain, which likely represents a general feature of this enzyme superfamily. Analysis of ligand-enzyme interactions as well as site-directed mutagenesis suggest Ser318 as one of the two acid-base catalysts. STRUCTURED SUMMARY OF PROTEIN INTERACTIONS: Rv1098c and Rv1098c bind by X-ray crystallography (View interaction). | ||
| - | + | Conformational changes upon ligand binding in the essential class II fumarase Rv1098c from Mycobacterium tuberculosis.,Mechaly AE, Haouz A, Miras I, Barilone N, Weber P, Shepard W, Alzari PM, Bellinzoni M FEBS Lett. 2012 Jun 4;586(11):1606-11. Epub 2012 May 3. PMID:22561013<ref>PMID:22561013</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 4adl" style="background-color:#fffaf0;"></div> | |
| - | + | ||
==See Also== | ==See Also== | ||
*[[Fumarase|Fumarase]] | *[[Fumarase|Fumarase]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| - | [[Category: | + | </StructureSection> |
| - | [[Category: Mycobacterium tuberculosis]] | + | [[Category: Large Structures]] |
| - | [[Category: Alzari | + | [[Category: Mycobacterium tuberculosis H37Rv]] |
| - | [[Category: Bellinzoni | + | [[Category: Alzari PM]] |
| - | [[Category: Cole | + | [[Category: Bellinzoni M]] |
| - | [[Category: Haouz | + | [[Category: Cole S]] |
| - | [[Category: Mechaly | + | [[Category: Haouz A]] |
| - | [[Category: Miras | + | [[Category: Mechaly AE]] |
| - | [[Category: Shepard | + | [[Category: Miras I]] |
| - | [[Category: Weber | + | [[Category: Shepard W]] |
| - | + | [[Category: Weber P]] | |
| - | + | ||
Current revision
Crystal structures of Rv1098c in complex with malate
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