This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
4bwm
From Proteopedia
(Difference between revisions)
| (5 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
| - | {{STRUCTURE_4bwm| PDB=4bwm | SCENE= }} | ||
| - | ===KlenTaq mutant in complex with a RNA/DNA hybrid=== | ||
| - | == | + | ==KlenTaq mutant in complex with a RNA/DNA hybrid== |
| - | [[4bwm]] is a 3 chain structure with sequence from [ | + | <StructureSection load='4bwm' size='340' side='right'caption='[[4bwm]], [[Resolution|resolution]] 1.75Å' scene=''> |
| - | [[ | + | == Structural highlights == |
| - | [[ | + | <table><tr><td colspan='2'>[[4bwm]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BWM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BWM FirstGlance]. <br> |
| - | [[Category: Bergen | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.749Å</td></tr> |
| - | [[Category: Blatter | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DCT:2,3-DIDEOXYCYTIDINE+5-TRIPHOSPHATE'>DCT</scene>, <scene name='pdbligand=DOC:2,3-DIDEOXYCYTIDINE-5-MONOPHOSPHATE'>DOC</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | [[Category: Diederichs | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bwm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bwm OCA], [https://pdbe.org/4bwm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bwm RCSB], [https://www.ebi.ac.uk/pdbsum/4bwm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bwm ProSAT]</span></td></tr> |
| - | [[Category: Marx | + | </table> |
| - | [[Category: Welte | + | == Function == |
| - | + | [https://www.uniprot.org/uniprot/DPO1_THEAQ DPO1_THEAQ] | |
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Unfaithful: The structure of a DNA-dependent DNA polymerase, whose parental ancestor has no significant reverse transcriptase activity, was obtained for the first time incorporating a nucleotide against an RNA template and then structurally characterized. The DNA polymerase variant has significant reverse transcriptase activity that allows its application in PCR-based molecular diagnostics. | ||
| + | |||
| + | Structure and Function of an RNA-Reading Thermostable DNA Polymerase.,Blatter N, Bergen K, Nolte O, Welte W, Diederichs K, Mayer J, Wieland M, Marx A Angew Chem Int Ed Engl. 2013 Sep 17. doi: 10.1002/anie.201306655. PMID:24106012<ref>PMID:24106012</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 4bwm" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[DNA polymerase 3D structures|DNA polymerase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Synthetic construct]] | ||
| + | [[Category: Thermus aquaticus]] | ||
| + | [[Category: Bergen K]] | ||
| + | [[Category: Blatter N]] | ||
| + | [[Category: Diederichs K]] | ||
| + | [[Category: Marx A]] | ||
| + | [[Category: Welte W]] | ||
Current revision
KlenTaq mutant in complex with a RNA/DNA hybrid
| |||||||||||
