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| | <StructureSection load='6s9f' size='340' side='right'caption='[[6s9f]], [[Resolution|resolution]] 1.97Å' scene=''> | | <StructureSection load='6s9f' size='340' side='right'caption='[[6s9f]], [[Resolution|resolution]] 1.97Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6s9f]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S9F OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6S9F FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6s9f]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S9F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6S9F FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.969Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">otk, CG8967 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6s9f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s9f OCA], [http://pdbe.org/6s9f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6s9f RCSB], [http://www.ebi.ac.uk/pdbsum/6s9f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6s9f ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6s9f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s9f OCA], [https://pdbe.org/6s9f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6s9f RCSB], [https://www.ebi.ac.uk/pdbsum/6s9f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6s9f ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PTK7_DROME PTK7_DROME]] Acts as a calcium-dependent, homophilic cell adhesion molecule that regulates neural recognition during the development of the nervous system. Component of the repulsive Plexin signaling response to regulate motor axon guidance at the embryonic stage. Also component of a receptor complex that is required in the adult visual system to innervate the lamina layer; specific targeting of R1-R6 axons.<ref>PMID:11604138</ref> <ref>PMID:1371458</ref> <ref>PMID:15456725</ref> | + | [https://www.uniprot.org/uniprot/PTK7_DROME PTK7_DROME] Acts as a calcium-dependent, homophilic cell adhesion molecule that regulates neural recognition during the development of the nervous system. Component of the repulsive Plexin signaling response to regulate motor axon guidance at the embryonic stage. Also component of a receptor complex that is required in the adult visual system to innervate the lamina layer; specific targeting of R1-R6 axons.<ref>PMID:11604138</ref> <ref>PMID:1371458</ref> <ref>PMID:15456725</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Drome]] | + | [[Category: Drosophila melanogaster]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Jones, E Y]] | + | [[Category: Jones EY]] |
| - | [[Category: Rozbesky, D]] | + | [[Category: Rozbesky D]] |
| - | [[Category: Ig-like domain]]
| + | |
| - | [[Category: Off-track]]
| + | |
| - | [[Category: Otk]]
| + | |
| - | [[Category: Signaling protein]]
| + | |
| - | [[Category: Signalling]]
| + | |
| Structural highlights
Function
PTK7_DROME Acts as a calcium-dependent, homophilic cell adhesion molecule that regulates neural recognition during the development of the nervous system. Component of the repulsive Plexin signaling response to regulate motor axon guidance at the embryonic stage. Also component of a receptor complex that is required in the adult visual system to innervate the lamina layer; specific targeting of R1-R6 axons.[1] [2] [3]
Publication Abstract from PubMed
The transmembrane protein OTK plays an essential role in plexin and Wnt signaling during Drosophila development. We have determined a crystal structure of the last three domains of the OTK ectodomain and found that OTK shows high conformational flexibility resulting from mobility at the interdomain interfaces. We failed to detect direct binding between Drosophila Plexin A (PlexA) and OTK, which was suggested previously. We found that, instead of PlexA, OTK directly binds semaphorin 1a. Our binding analyses further revealed that glycosaminoglycans, heparin and heparan sulfate, are ligands for OTK and thus may play a role in the Sema1a-PlexA axon guidance system.
Drosophila OTK Is a Glycosaminoglycan-Binding Protein with High Conformational Flexibility.,Rozbesky D, Monistrol J, Jain V, Hillier J, Padilla-Parra S, Jones EY Structure. 2020 Mar 13. pii: S0969-2126(20)30072-1. doi:, 10.1016/j.str.2020.02.008. PMID:32187531[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Winberg ML, Tamagnone L, Bai J, Comoglio PM, Montell D, Goodman CS. The transmembrane protein Off-track associates with Plexins and functions downstream of Semaphorin signaling during axon guidance. Neuron. 2001 Oct 11;32(1):53-62. doi: 10.1016/s0896-6273(01)00446-9. PMID:11604138 doi:http://dx.doi.org/10.1016/s0896-6273(01)00446-9
- ↑ Pulido D, Campuzano S, Koda T, Modolell J, Barbacid M. Dtrk, a Drosophila gene related to the trk family of neurotrophin receptors, encodes a novel class of neural cell adhesion molecule. EMBO J. 1992 Feb;11(2):391-404. PMID:1371458
- ↑ Cafferty P, Yu L, Rao Y. The receptor tyrosine kinase Off-track is required for layer-specific neuronal connectivity in Drosophila. Development. 2004 Nov;131(21):5287-95. doi: 10.1242/dev.01406. Epub 2004 Sep 29. PMID:15456725 doi:http://dx.doi.org/10.1242/dev.01406
- ↑ Rozbesky D, Monistrol J, Jain V, Hillier J, Padilla-Parra S, Jones EY. Drosophila OTK Is a Glycosaminoglycan-Binding Protein with High Conformational Flexibility. Structure. 2020 Mar 13. pii: S0969-2126(20)30072-1. doi:, 10.1016/j.str.2020.02.008. PMID:32187531 doi:http://dx.doi.org/10.1016/j.str.2020.02.008
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