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4ke2

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==Crystal structure of the hyperactive Type I antifreeze from winter flounder==
==Crystal structure of the hyperactive Type I antifreeze from winter flounder==
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<StructureSection load='4ke2' size='340' side='right' caption='[[4ke2]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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<StructureSection load='4ke2' size='340' side='right'caption='[[4ke2]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4ke2]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseam Pseam]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KE2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KE2 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4ke2]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudopleuronectes_americanus Pseudopleuronectes americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KE2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KE2 FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ke2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ke2 OCA], [http://pdbe.org/4ke2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ke2 RCSB], [http://www.ebi.ac.uk/pdbsum/4ke2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ke2 ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ke2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ke2 OCA], [https://pdbe.org/4ke2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ke2 RCSB], [https://www.ebi.ac.uk/pdbsum/4ke2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ke2 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/ANPM_PSEAM ANPM_PSEAM] Contributes to protect fish blood from freezing at subzero sea water temperatures. Lowers the blood freezing point by about 1.1 degrees at a concentration of 0.1 mg/ml, and by about 1.5 degrees at a concentration of 0.2 mg/ml. Binds to nascent ice crystals and prevents further growth.<ref>PMID:15141201</ref> <ref>PMID:15716269</ref> <ref>PMID:18225917</ref> <ref>PMID:24531972</ref>
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When polypeptide chains fold into a protein, hydrophobic groups are compacted in the center with exclusion of water. We report the crystal structure of an alanine-rich antifreeze protein that retains ~400 waters in its core. The putative ice-binding residues of this dimeric, four-helix bundle protein point inwards and coordinate the interior waters into two intersecting polypentagonal networks. The bundle makes minimal protein contacts between helices, but is stabilized by anchoring to the semi-clathrate water monolayers through backbone carbonyl groups in the protein interior. The ordered waters extend outwards to the protein surface and likely are involved in ice binding. This protein fold supports both the anchored-clathrate water mechanism of antifreeze protein adsorption to ice and the water-expulsion mechanism of protein folding.
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An antifreeze protein folds with an interior network of more than 400 semi-clathrate waters.,Sun T, Lin FH, Campbell RL, Allingham JS, Davies PL Science. 2014 Feb 14;343(6172):795-8. doi: 10.1126/science.1247407. PMID:24531972<ref>PMID:24531972</ref>
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==See Also==
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*[[Antifreeze protein 3D structures|Antifreeze protein 3D structures]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4ke2" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Pseam]]
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[[Category: Large Structures]]
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[[Category: Allingham, J S]]
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[[Category: Pseudopleuronectes americanus]]
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[[Category: Campbell, R L]]
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[[Category: Allingham JS]]
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[[Category: Davies, P L]]
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[[Category: Campbell RL]]
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[[Category: Lin, F H]]
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[[Category: Davies PL]]
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[[Category: Sun, T]]
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[[Category: Lin F-H]]
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[[Category: Antifreeze protein]]
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[[Category: Sun T]]
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[[Category: Dimeric alpha-helical bundle]]
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Current revision

Crystal structure of the hyperactive Type I antifreeze from winter flounder

PDB ID 4ke2

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