This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


5yiy

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (10:23, 27 March 2024) (edit) (undo)
 
Line 1: Line 1:
==Crystal structure of D175A mutant of Rv3272 from Mycobacterium tuberculosis==
==Crystal structure of D175A mutant of Rv3272 from Mycobacterium tuberculosis==
-
<StructureSection load='5yiy' size='340' side='right' caption='[[5yiy]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
+
<StructureSection load='5yiy' size='340' side='right'caption='[[5yiy]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[5yiy]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YIY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YIY FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[5yiy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YIY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5YIY FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.504&#8491;</td></tr>
-
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Rv3272 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5yiy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yiy OCA], [http://pdbe.org/5yiy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yiy RCSB], [http://www.ebi.ac.uk/pdbsum/5yiy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yiy ProSAT]</span></td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5yiy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yiy OCA], [https://pdbe.org/5yiy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5yiy RCSB], [https://www.ebi.ac.uk/pdbsum/5yiy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5yiy ProSAT]</span></td></tr>
</table>
</table>
-
<div style="background-color:#fffaf0;">
+
== Function ==
-
== Publication Abstract from PubMed ==
+
[https://www.uniprot.org/uniprot/COATR_MYCTU COATR_MYCTU] Probably involved in fatty acid metabolism. Binds to fatty acyl-CoAs of varying carbon chain lengths, with the highest binding affinity for palmitoyl-CoA (C16:0). In vitro, alters the cell wall lipid profile and protects mycobacteria from acidic, oxidative and antibiotic stress. May play a significant role in host-pathogen interaction.<ref>PMID:30342240</ref>
-
The availability of complete genome sequence of Mycobacterium tuberculosis has provided an important tool to understand the mycobacterial biology with respect to host-pathogen interaction, which is an unmet need of the hour owing to continuous increasing drug resistance. Hypothetical proteins are often an overlooked pool though half the genome encodes for such proteins of unknown function that could potentially play vital roles in mycobacterial biology. In this context, we report the structural and functional characterization of the hypothetical protein Rv3272. Sequence analysis classifies Rv3272 as a Family III CoA transferase with the classical two domain structure and conserved Aspartate residue (D175). The crystal structure of the wild type protein (2.2A) demonstrated the associated inter-locked dimer while that of the D175A mutant co-crystallized with octanoyl-CoA demonstrated relative movement between the two domains. Isothermal titration calorimetry studies indicate that Rv3272 binds to fatty acyl-CoAs of varying carbon chain lengths, with palmitoyl-CoA (C16:0) exhibiting maximum affinity. To determine the functional relevance of Rv3272 in mycobacterial biology, we ectopically expressed Rv3272 in M. smegmatis and assessed that its expression encodes significant alteration in cell surface with marked differences in triacylglycerol accumulation. Additionally, Rv3272 expression protects mycobacteria from acidic, oxidative and antibiotic stress under in vitro conditions. Taken together, these studies indicate a significant role for Rv3272 in host-pathogen interaction.
+
-
 
+
-
Rv3272 encodes a novel Family III CoA transferase that alters the cell wall lipid profile and protects mycobacteria from acidic and oxidative stress.,Karade SS, Pandey S, Ansari A, Das S, Tripathi S, Arora A, Chopra S, Pratap JV, Dasgupta A Biochim Biophys Acta Proteins Proteom. 2018 Oct 17. pii: S1570-9639(18)30180-8., doi: 10.1016/j.bbapap.2018.10.011. PMID:30342240<ref>PMID:30342240</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 5yiy" style="background-color:#fffaf0;"></div>
+
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Myctu]]
+
[[Category: Large Structures]]
-
[[Category: Karade, S S]]
+
[[Category: Mycobacterium tuberculosis H37Rv]]
-
[[Category: Pratap, J V]]
+
[[Category: Karade SS]]
-
[[Category: Coa transferase]]
+
[[Category: Pratap JV]]
-
[[Category: D175a]]
+
-
[[Category: Mycobacterium]]
+
-
[[Category: Rv3272]]
+
-
[[Category: Transferase]]
+

Current revision

Crystal structure of D175A mutant of Rv3272 from Mycobacterium tuberculosis

PDB ID 5yiy

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5yiy"
Personal tools