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Chaperonin, Sandbox transition test

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<StructureSection load='1pcq' size='350' side='right' caption='E. coli GroEL/GroES complex with ADP, AlF3, Mg+2 and K+ ions (PDB entry [[1pcq]])' scene='44/445432/Cv/1'>
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==Your Heading Here (maybe something like 'Structure')==
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[[Image:1pcq.png|left|200px|thumb|Crystal Structure of Chaperonin, [[1pcq]]]]
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<StructureSection load='1stp' size='340' side='right' caption='Caption for this structure' scene=''>
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'''Chaperonins''' (CPN) are oligomeric proteins that mediate the folding of polypeptide chains. '''Group I CPN''' are found in bacteria, chloroplasts and mitochondria. For an introductory overview, see [http://en.wikipedia.org/wiki/Chaperonins Chaperonins in Wikipedia].
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This is a default text for your page '''Sandbox transition test'''. Click above on '''edit this page''' to modify. Be careful with the &lt; and &gt; signs.
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You may include any references to papers as in: the use of JSmol in Proteopedia <ref>DOI 10.1002/ijch.201300024</ref> or to the article describing Jmol <ref>PMID:21638687</ref> to the rescue.
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The most characterized CPN are in the GroEL/GroES complex from ''Escherichia coli'' and CPN60/CPN10 from ''Thermus thermophilus''.<ref>PMID:18987317</ref> The larger subunit (GroEL, CPN60) contains 3 domains: apical, intermediate and equatorial domain. The apical domain is the one which binds the polypeptide substrate. The equatorial domains binds the nucleotide. '''Group II CPNs''' are found in eukaryotic cytosol and archaea. '''Thermosome''' is a CPN complex found in archaea.<ref>PMID:9546398</ref> '''CCT''' is a CPN complex found in eukarya.<ref>PMID:22503819</ref>
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== Function ==
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*<scene name='44/445432/Cv/2'>E. coli GroEL/GroES complex</scene> (GroEL in green, GroES in magenta, PDB entry [[1pcq]]).
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*See also [[Chaperones]].
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</StructureSection>
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== 3D Structures of Chaperonin ==
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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== Disease ==
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{{#tree:id=OrganizedByTopic|openlevels=0|
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<br>
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Load 1eve and save with standard defaults - no reload standard display YES YES transition <scene name='10/1050327/Sb_transition_default/1'>Default</scene>
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<br>
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blow up
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always reload YES YES <scene name='10/1050327/Sb_transition_default/2'>default blow up</scene>
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<br>
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no reload YES YES <scene name='10/1050327/Sb_transition_default/3'>NO Reload Blow up</scene>
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== Relevance ==
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*'''Group I'''
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== Structural highlights ==
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*''Large subunit''
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The X-ray structure of the E2020-''Tc''AChE complex shows that E2020 has a <scene name='1eve/E2020_close_up_with_84_279/13'>unique orientation</scene> along the active-site gorge, extending from the anionic subsite (<scene name='1eve/E2020_close_up_with_84lbld/7'>W84</scene>) of the active site, at the bottom, to the peripheral anionic site (<scene name='1eve/E2020_close_up_with_84_279lbld/5'>near W279</scene>), at the top, via aromatic stacking interactions with conserved aromatic acid residues. E2020 does not, however, interact directly with either the catalytic triad or the 'oxyanion hole' but only <scene name='1eve/E20_interactionshown/8'>indirectly via solvent molecules</scene>.
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<br>
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**[[3m6c]] – MtGroEL1 apical domain – ''Mycobacterium tuberculosis''<BR />
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</StructureSection>
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**[[1sjp]], [[3rtk]] – MtGroEL2 residues 42-539<BR />
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**[[3fbh]], [[2eu1]], [[1j4z]], [[1kpo]], [[1oel]], [[1grl]], [[2yey]] – EcGroEL (mutant) - ''Escherichia coli''<BR />
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**[[3e76]], [[2nwc]], [[1xck]], [[1ss8]], [[4hel]] – EcGroEL<BR />
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**[[3c9v]], [[3cau]], [[2ynj]] – EcGroEL – Cryo EM<BR />
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**[[2c7e]], [[1gr5]], [[4aaq]] - EcGroEL (mutant) – Cryo EM<BR />
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**[[1dk7]], [[1la1]] - EcGroEL apical domain<br />
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**[[1fy9]], [[1fya]], [[1kid]], [[1jon]], [[3vz6]], [[3vz7]], [[3vz8]] – EcGroEL apical domain (mutant) <BR />
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**[[1srv]] – TtCPN60 apical domain - ''Thermus thermophilus''<BR />
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**[[3osx]] - CPN60 apical domain – ''Xenorhabdus nematophila''<br />
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**[[1iok]] – CPN60 – ''Paracoccus denitrificans''
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*''Large subunit binary complex''
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**[[1kp8]] - EcGroEL (mutant) + ATP<BR />
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**[[4aar]], [[4aas]], [[4aau]], [[4ab2]], [[4ab3]] - EcGroEL (mutant) + ATP – Cryo EM<br />
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**[[1sx3]] - EcGroEL + ATP<BR />
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**[[1sx4]] - EcGroEL + ADP<BR />
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**[[4ki8]] - EcGroEL (mutant) + ADP<br />
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**[[1mnf]] – EcGroEL + polypeptide<BR />
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**[[2cgt]] – EcGroEL + capsid assbly protein GP31 – EM<BR />
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**[[1dkd]] - EcGroEL apical domain + polypeptide
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*''Small subunit''
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**[[3nx6]] – GroES residues 40-134 (mutant) – ''Xanthomonas oryzae''<BR />
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**[[1wnr]] – TtCPN10 residues 1-94<BR />
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**[[1p3h]], [[1hx5]] – MtCPN10<BR />
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**[[1p82]], [[1p83]] - MtCPN10 residues 1-25 - NMR<BR />
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**[[1egs]] - GroES residues 19-27 - NMR
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*''Large + small subunit''
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**[[1svt]], [[1pcq]] - EcGroEL + GroES<BR />
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**[[1pf9]], [[1aon]], [[4pko]] - EcGroEL + GroES + ADP<BR />
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**[[3wvl]] - EcGroEL (mutant) + GroES + ATP<br />
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**[[2c7c]], [[2c7d]] - EcGroEL + GroES – Cryo EM<BR />
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**[[1gru]] - EcGroEL + GroES + ATP + ADP – Cryo EM<BR />
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**[[3zpz]], [[3zq0]], [[3zq1]] - EcGroEL + GroES + ADP – Cryo EM<br />
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**[[1we3]] – TtCPN60 + CPN10<BR />
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**[[1wf4]], [[4v4o]], [[4pkn]] - TtCPN60 + CPN10 + ADP
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*'''Group II'''
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**[[3izh]], [[3izi]], [[3izj]], [[3izk]], [[3izl]], [[3izm]], [[3izn]], [[3los]], [[3iyf]], [[3j3x]] – MmCPN – ''Methanococcus maripaludis'' – EM<BR />
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**[[3kfb]], [[3kfe]], [[3kfk]] – MmCPN<BR />
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**[[3j02]], [[3j03]] – MmCPN (mutant) – Cryo EM<br />
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**[[3ruq]] - MmCPN + ADP<br />
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**[[3rus]] - MmCPN (mutant) + ADP<br />
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**[[3ruw]] - MmCPN (mutant) + ADP-AlF3<br />
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**[[3ruv]] - MmCPN (mutant) + ATP analog<br />
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*'''CCT'''
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**[[2xsm]], [[4b2t]] – bCPN CCT – bovine<BR />
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**[[3p9d]], [[3p9e]], [[4d8q]], [[4d8r]], [[4aol]], [[4apk]] – CPN CCT - yeast<br />
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**[[3iyg]], [[4a0o]] - bCPN CCT – Cryo EM<BR />
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**[[4a0v]] - bCPN CCT + AMPPNP – Cryo EM<br />
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**[[4a0w]] - bCPN CCT + ADP + AlF3 – Cryo EM<br />
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**[[4a13]] - bCPN CCT + ADP – Cryo EM<br />
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**[[3ktt]] - bCPN CCT β subunit – Cryo EM<BR />
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**[[1gml]], [[1gn1]] - CPN CCT γ subunit apical domain – mouse
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*'''Hsp33'''
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See [[Heat Shock Proteins]]
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*'''Thermosome'''
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**[[1a6d]] - TaTherm α+β subunits – ''Thermoplasma acidophilum''<BR />
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**[[1a6e]] - TaTherm α+β subunits + ADP<BR />
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**[[1ass]], [[1asx]] - TaTherm α apical domain<BR />
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**[[1e0r]] – TaTherm β apical domain<br />
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**[[3ko1]] – AtTherm α subunit– ''Acidianus tengchongensis''<br />
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**[[3j1b]], [[3j1c]], [[3j1e]] - AtTherm α subunit – Cryo EM <br />
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**[[3j1f]] - AtTherm β subunit + ATP – Cryo EM <br />
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**[[3aq1]] – Therm – ''Methanococcoides burtonii''<br />
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**[[1q2v]], [[1q3r]] – TkTherm α subunit (mutant) – ''Thermococcus KS-1''<br />
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**[[1q3q]] - TkTherm α subunit (mutant) + AMP-PNP<br />
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**[[1q3s]] - TkTherm α subunit (mutant) + ADP<br />
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**[[1lep]] – CPN-10 – ''Mycobacterium leprae''
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}}
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== References ==
== References ==
<references/>
<references/>
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[[Category:Topic Page]]
 

Revision as of 10:35, 8 March 2026

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Caption for this structure

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References

  1. ↑ Hanson, R. M., Prilusky, J., Renjian, Z., Nakane, T. and Sussman, J. L. (2013), JSmol and the Next-Generation Web-Based Representation of 3D Molecular Structure as Applied to Proteopedia. Isr. J. Chem., 53:207-216. doi:http://dx.doi.org/10.1002/ijch.201300024
  2. ↑ Herraez A. Biomolecules in the computer: Jmol to the rescue. Biochem Mol Biol Educ. 2006 Jul;34(4):255-61. doi: 10.1002/bmb.2006.494034042644. PMID:21638687 doi:10.1002/bmb.2006.494034042644
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