Deoxycytidine kinase
From Proteopedia
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'''Deoxycytidine kinase''' (DCK) is required for the phosphorylation of deoxyribonucleosides dC, dG and dA (but not dT and dU) and nucleosides analogs which are used as antiviral and chemotherapeutic agents. <ref>PMID:20614893</ref> | '''Deoxycytidine kinase''' (DCK) is required for the phosphorylation of deoxyribonucleosides dC, dG and dA (but not dT and dU) and nucleosides analogs which are used as antiviral and chemotherapeutic agents. <ref>PMID:20614893</ref> | ||
| + | '''Deoxyadenosine/deoxycytidine kinase''' (DACK) substrates ar cytidine, adenosine, arabinosylcytosine and arabinosyladenine <ref>PMID:6251049</ref>. | ||
== Relevance == | == Relevance == | ||
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== Structural highlights == | == Structural highlights == | ||
| - | The ability of DCK to phosphorylate multiple nucleosides arises from the different conformations which the active site glutamic acid can assume.<ref>PMID:12808445</ref> | + | The ability of <scene name='54/543346/Cv/4'>DCK to phosphorylate multiple nucleosides arises from the different conformations which the active site glutamic acid can assume</scene> (active site glutamates are in yellow).<ref>PMID:12808445</ref> |
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== 3D Structures of deoxycytidine kinase == | == 3D Structures of deoxycytidine kinase == | ||
| + | [[Deoxycytidine kinase 3D structures]] | ||
| - | + | </StructureSection> | |
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| - | **[[2qrn]] – hDCK + UDP + CMP<br /> | ||
| - | **[[2qro]] – hDCK + UDP + AMP<br /> | ||
| - | **[[3ipx]] – hDCK + ADP + inhibitor<br /> | ||
| - | **[[3ipy]] – hDCK + inhibitor<br /> | ||
| - | **[[3qej]] – hDCK (mutant) + UDP <br /> | ||
| - | **[[4jlj]], [[4jlk]], [[4jlm]], [[4jln]], [[4l5b]], [[4q18]], [[4q19]], [[4q1a]], [[4q1b]], [[4q1c]], [[4q1d]], [[4q1e]], [[4q1f]] - hDCK (mutant) + UDP + inhibitor<br /> | ||
| - | **[[3mjr]] – hDCK + UDP + hydroxyethoxymethylguanine<br /> | ||
| - | }} | ||
== References == | == References == | ||
<references/> | <references/> | ||
[[Category:Topic Page]] | [[Category:Topic Page]] | ||
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References
- ↑ Hazra S, Ort S, Konrad M, Lavie A. Structural and kinetic characterization of human deoxycytidine kinase variants able to phosphorylate 5-substituted deoxycytidine and thymidine analogues . Biochemistry. 2010 Aug 10;49(31):6784-90. PMID:20614893 doi:10.1021/bi100839e
- ↑ Møllgaard H. Deoxyadenosine/deoxycytidine kinase from Bacillus subtilis. Purification, characterization, and physiological function. J Biol Chem. 1980 Sep 10;255(17):8216-20 PMID:6251049
- ↑ Sabini E, Ort S, Monnerjahn C, Konrad M, Lavie A. Structure of human dCK suggests strategies to improve anticancer and antiviral therapy. Nat Struct Biol. 2003 Jul;10(7):513-9. PMID:12808445 doi:http://dx.doi.org/10.1038/nsb942
