Forms of DNA
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| - | + | == A comparative representation of the three forms of DNA == | |
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''Sources'' <ref>Nucleic Acid Canonical Coordinates http://nucleix.mbu.iisc.ac.in/nacc/index.html</ref> | ''Sources'' <ref>Nucleic Acid Canonical Coordinates http://nucleix.mbu.iisc.ac.in/nacc/index.html</ref> | ||
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</jmol> | </jmol> | ||
| - | + | == Helical Parameters of the three forms of DNA == | |
DNA is a very flexible molecule and has the ability to exist in various forms based on the environmental conditions. Naturally occurring DNA double helices are classified into A, B and Z-types. A and B-forms of DNA are the right handed forms whereas [[Z-DNA]] is the left handed form. When hydrated the DNA generally assumes B-form. The A conformation is found when there is little water to interact with the helix and is also the conformation adopted by the RNA. The formation of Z-DNA occurs with the methylation of deoxycytosine residues and also during transcription where negative supercoiling stabilizes it. | DNA is a very flexible molecule and has the ability to exist in various forms based on the environmental conditions. Naturally occurring DNA double helices are classified into A, B and Z-types. A and B-forms of DNA are the right handed forms whereas [[Z-DNA]] is the left handed form. When hydrated the DNA generally assumes B-form. The A conformation is found when there is little water to interact with the helix and is also the conformation adopted by the RNA. The formation of Z-DNA occurs with the methylation of deoxycytosine residues and also during transcription where negative supercoiling stabilizes it. | ||
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|Diameter of helix [Å]||align="right"| 23||align="right"| 20||align="right"| 18 | |Diameter of helix [Å]||align="right"| 23||align="right"| 20||align="right"| 18 | ||
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| - | |Glycosidic bond configuration<br | + | |Glycosidic bond configuration<br>dA,dT,dC<br>dG ||align="center"| <br>anti<br>anti ||align="center"| <br>anti<br>anti ||align="center"| <br>anti<br>syn |
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| - | |Sugar pucker<br | + | |[[Sugar ring pucker|Sugar pucker]]<br>dA,dT,dC<br>dG ||align="center"| <br>C3'-endo<br>C3'-endo ||align="center"|<br> C2'-endo<br>C2'-endo ||align="center"| <br>C2'-endo<br>C3'-endo |
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| - | |Intrastrand phosphate-phosphate distance [Å] <br | + | |Intrastrand phosphate-phosphate distance [Å] <br>dA,dT,dC<br>dG ||align="center"| <br>5.9<br>5.9||align="center"| <br>7.0<br>7.0||align="center"| <br>7.0<br> 5.9 |
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|colspan="4"|''Sources: <ref name="Rich1984">PMID:6383204</ref><ref name="Rich1979">PMID: 514347</ref><ref> Sinden, Richard R (1994-01-15). ''DNA structure and function'' (1st ed.). Academic Press. pp. 398. ISBN 0-12-645750-6.</ref> | |colspan="4"|''Sources: <ref name="Rich1984">PMID:6383204</ref><ref name="Rich1979">PMID: 514347</ref><ref> Sinden, Richard R (1994-01-15). ''DNA structure and function'' (1st ed.). Academic Press. pp. 398. ISBN 0-12-645750-6.</ref> | ||
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</StructureSection> | </StructureSection> | ||
| - | == References== | + | == See also == |
| + | * [[DNA - History of the discovery and current applications (Hebrew)]] | ||
| + | * [[DNA]] | ||
| + | * [[Z-DNA]] | ||
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| + | == References == | ||
<references/> | <references/> | ||
Current revision
Contents |
A comparative representation of the three forms of DNA
Sources [1]
| A-DNA | B-DNA | Z-DNA | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
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Change rendering:
Helical Parameters of the three forms of DNA
DNA is a very flexible molecule and has the ability to exist in various forms based on the environmental conditions. Naturally occurring DNA double helices are classified into A, B and Z-types. A and B-forms of DNA are the right handed forms whereas Z-DNA is the left handed form. When hydrated the DNA generally assumes B-form. The A conformation is found when there is little water to interact with the helix and is also the conformation adopted by the RNA. The formation of Z-DNA occurs with the methylation of deoxycytosine residues and also during transcription where negative supercoiling stabilizes it.
| Parameter | A-DNA | B-DNA | Z-DNA |
|---|---|---|---|
| Helix sense | right-handed | right-handed | left-handed |
| Residues per turn | 11 | 10.5 | 12 |
| Axial rise [Å] | 2.55 | 3.4 | 3.7 |
| Helix pitch(°) | 28 | 34 | 45 |
| Base pair tilt(°) | 20 | −6 | 7 |
| Rotation per residue (°) | 33 | 36 | -30 |
| Diameter of helix [Å] | 23 | 20 | 18 |
| Glycosidic bond configuration dA,dT,dC dG | anti anti | anti anti | anti syn |
| Sugar pucker dA,dT,dC dG | C3'-endo C3'-endo | C2'-endo C2'-endo | C2'-endo C3'-endo |
| Intrastrand phosphate-phosphate distance [Å] dA,dT,dC dG | 5.9 5.9 | 7.0 7.0 | 7.0 5.9 |
| Sources: [2][3][4] | |||
Structural Transformation between A and B DNA
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See also
References
- ↑ Nucleic Acid Canonical Coordinates http://nucleix.mbu.iisc.ac.in/nacc/index.html
- ↑ Rich A, Nordheim A, Wang AH. The chemistry and biology of left-handed Z-DNA. Annu Rev Biochem. 1984;53:791-846. PMID:6383204 doi:http://dx.doi.org/10.1146/annurev.bi.53.070184.004043
- ↑ Wang AH, Quigley GJ, Kolpak FJ, Crawford JL, van Boom JH, van der Marel G, Rich A. Molecular structure of a left-handed double helical DNA fragment at atomic resolution. Nature. 1979 Dec 13;282(5740):680-6. PMID:514347
- ↑ Sinden, Richard R (1994-01-15). DNA structure and function (1st ed.). Academic Press. pp. 398. ISBN 0-12-645750-6.
- ↑ Krebs WG, Gerstein M. The morph server: a standardized system for analyzing and visualizing macromolecular motions in a database framework. Nucleic Acids Res. 2000 Apr 15;28(8):1665-75. PMID:10734184
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