User:Karl E. Zahn/Sandbox RB69 1/palm-domain
From Proteopedia
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(New page: The palm domain of RB69 DNA pol (residues 383-468, 573-729) contains the catalytic motifs that allow coordination of divalent cations required for the two-metal ion mechanism employed by p...) |
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| - | The | + | The two-metal ion mechanism employed during the pol catalytic cycle relies on coordination of divalent cations by conserved motifs in the palm domain of DNA pols <ref>PMID:8341661</ref><ref>PMID:9440688</ref>. The palm domain of RB69 DNA pol (residues 383-468, 573-729) contains such motifs (VIYGDTD) that are particularly appropriate for family B DNA pols. Interestingly, the Thr (622) residue in this sequence must obtain a high energy conformation in order to correctly orient the Asp residues. This property is evident in Ramachandran plots of RB69 DNA pol where T622 is an obvious out-layer. |
Revision as of 19:40, 22 April 2009
The two-metal ion mechanism employed during the pol catalytic cycle relies on coordination of divalent cations by conserved motifs in the palm domain of DNA pols [1][2]. The palm domain of RB69 DNA pol (residues 383-468, 573-729) contains such motifs (VIYGDTD) that are particularly appropriate for family B DNA pols. Interestingly, the Thr (622) residue in this sequence must obtain a high energy conformation in order to correctly orient the Asp residues. This property is evident in Ramachandran plots of RB69 DNA pol where T622 is an obvious out-layer.
