1l8w

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(New page: 200px<br /><applet load="1l8w" size="450" color="white" frame="true" align="right" spinBox="true" caption="1l8w, resolution 2.30&Aring;" /> '''Crystal Structure of...)
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[[Image:1l8w.gif|left|200px]]<br /><applet load="1l8w" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1l8w, resolution 2.30&Aring;" />
 
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'''Crystal Structure of Lyme Disease Variable Surface Antigen VlsE of Borrelia burgdorferi'''<br />
 
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==Overview==
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==Crystal Structure of Lyme Disease Variable Surface Antigen VlsE of Borrelia burgdorferi==
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VlsE is an outer surface lipoprotein of Borrelia burgdorferi that, undergoes antigenic variation through an elaborate gene conversion, mechanism and is thought to play a major role in the immune response to, the Lyme disease borellia. The crystal structure of recombinant variant, protein VlsE1 at 2.3-A resolution reveals that the six variable regions, form loop structures that constitute most of the membrane distal surface, of VlsE, covering the predominantly alpha-helical, invariant regions of, the protein. The surface localization of the variable amino acid segments, appears to protect the conserved regions from interaction with antibodies, and hence may contribute to immune evasion.
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<StructureSection load='1l8w' size='340' side='right'caption='[[1l8w]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1l8w]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Borreliella_burgdorferi Borreliella burgdorferi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L8W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1L8W FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1l8w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l8w OCA], [https://pdbe.org/1l8w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1l8w RCSB], [https://www.ebi.ac.uk/pdbsum/1l8w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1l8w ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/O06878_BORBG O06878_BORBG] The Vlp and Vsp proteins are antigenically distinct proteins, only one vlp or vsp gene is transcriptionally active at any one time. Switching between these genes is a mechanism of host immune response evasion.[RuleBase:RU363105]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l8/1l8w_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1l8w ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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VlsE is an outer surface lipoprotein of Borrelia burgdorferi that undergoes antigenic variation through an elaborate gene conversion mechanism and is thought to play a major role in the immune response to the Lyme disease borellia. The crystal structure of recombinant variant protein VlsE1 at 2.3-A resolution reveals that the six variable regions form loop structures that constitute most of the membrane distal surface of VlsE, covering the predominantly alpha-helical, invariant regions of the protein. The surface localization of the variable amino acid segments appears to protect the conserved regions from interaction with antibodies and hence may contribute to immune evasion.
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==About this Structure==
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Crystal structure of Lyme disease variable surface antigen VlsE of Borrelia burgdorferi.,Eicken C, Sharma V, Klabunde T, Lawrenz MB, Hardham JM, Norris SJ, Sacchettini JC J Biol Chem. 2002 Jun 14;277(24):21691-6. Epub 2002 Mar 28. PMID:11923306<ref>PMID:11923306</ref>
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1L8W is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Borrelia_burgdorferi Borrelia burgdorferi]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1L8W OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structure of Lyme disease variable surface antigen VlsE of Borrelia burgdorferi., Eicken C, Sharma V, Klabunde T, Lawrenz MB, Hardham JM, Norris SJ, Sacchettini JC, J Biol Chem. 2002 Jun 14;277(24):21691-6. Epub 2002 Mar 28. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11923306 11923306]
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</div>
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[[Category: Borrelia burgdorferi]]
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<div class="pdbe-citations 1l8w" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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== References ==
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[[Category: Eicken, C.]]
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<references/>
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[[Category: Hardham, J.M.]]
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__TOC__
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[[Category: Klabunde, T.]]
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</StructureSection>
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[[Category: Lawrenz, M.B.]]
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[[Category: Borreliella burgdorferi]]
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[[Category: Norris, S.J.]]
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[[Category: Large Structures]]
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[[Category: Sacchettini, J.C.]]
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[[Category: Eicken C]]
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[[Category: Sharma, V.]]
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[[Category: Hardham JM]]
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[[Category: variable surface protein]]
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[[Category: Klabunde T]]
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[[Category: vmp-like sequence]]
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[[Category: Lawrenz MB]]
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[[Category: Norris SJ]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 02:39:47 2007''
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[[Category: Sacchettini JC]]
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[[Category: Sharma V]]

Current revision

Crystal Structure of Lyme Disease Variable Surface Antigen VlsE of Borrelia burgdorferi

PDB ID 1l8w

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