1q5r

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(New page: 200px<br /><applet load="1q5r" size="450" color="white" frame="true" align="right" spinBox="true" caption="1q5r, resolution 3.10&Aring;" /> '''The Rhodococcus 20S ...)
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[[Image:1q5r.gif|left|200px]]<br /><applet load="1q5r" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1q5r, resolution 3.10&Aring;" />
 
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'''The Rhodococcus 20S proteasome with unprocessed pro-peptides'''<br />
 
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==Overview==
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==The Rhodococcus 20S proteasome with unprocessed pro-peptides==
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To understand the role of the pro-peptide in proteasome assembly, we have, determined structures of the Rhodococcus proteasome and a mutant form that, prevents the autocatalytic removal of its pro-peptides. The structures, reveal that the pro-peptide acts as an assembly-promoting factor by, linking its own beta-subunit to two adjacent alpha-subunits, thereby, providing a molecular explanation for the observed kinetics of proteasome, assembly. The Rhodococcus proteasome has been found to have a, substantially smaller contact region between alpha-subunits compared to, those regions in the proteasomes of Thermoplasma, yeast, and mammalian, cells, suggesting that a smaller contact area between alpha-subunits is, likely the structural basis for the Rhodococcus alpha-subunits not, assembling into alpha-rings when expressed alone. Analysis of all, available beta-subunit structures shows that the contact area between, beta-subunits within a beta-ring is not sufficient for beta-ring, self-assembly without the additional contact provided by the alpha-ring., This appears to be a fail-safe mechanism ensuring that the active sites on, the beta-subunits are activated only after proteasome assembly is, complete.
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<StructureSection load='1q5r' size='340' side='right'caption='[[1q5r]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1q5r]] is a 14 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodococcus_erythropolis Rhodococcus erythropolis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q5R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Q5R FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1q5r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q5r OCA], [https://pdbe.org/1q5r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1q5r RCSB], [https://www.ebi.ac.uk/pdbsum/1q5r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1q5r ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PSB1_RHOER PSB1_RHOER] Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation. The R.erythropolis proteasomes are able to cleave oligopeptides after Tyr, Phe and Leu, very poorly after Arg but not after Glu. Thus, displays chymotrypsin-like activity, low trypsin-like activity but no caspase-like activity.[HAMAP-Rule:MF_02113]<ref>PMID:7583123</ref> <ref>PMID:9000518</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q5/1q5r_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1q5r ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1Q5R is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rhodococcus_erythropolis Rhodococcus erythropolis]. Active as [http://en.wikipedia.org/wiki/Proteasome_endopeptidase_complex Proteasome endopeptidase complex], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.25.1 3.4.25.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1Q5R OCA].
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*[[Proteasome 3D structures|Proteasome 3D structures]]
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== References ==
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==Reference==
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<references/>
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Crystal structures of the Rhodococcus proteasome with and without its pro-peptides: implications for the role of the pro-peptide in proteasome assembly., Kwon YD, Nagy I, Adams PD, Baumeister W, Jap BK, J Mol Biol. 2004 Jan 2;335(1):233-45. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14659753 14659753]
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__TOC__
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[[Category: Proteasome endopeptidase complex]]
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</StructureSection>
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[[Category: Protein complex]]
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[[Category: Large Structures]]
[[Category: Rhodococcus erythropolis]]
[[Category: Rhodococcus erythropolis]]
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[[Category: Adams, P.D.]]
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[[Category: Adams PD]]
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[[Category: Baumeister, W.]]
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[[Category: Baumeister W]]
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[[Category: Jap, B.K.]]
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[[Category: Jap BK]]
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[[Category: Kwon, Y.D.]]
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[[Category: Kwon YD]]
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[[Category: Nagy, I.]]
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[[Category: Nagy I]]
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[[Category: inter-subunit contacts]]
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[[Category: pro-peptide]]
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[[Category: proteasome assembly]]
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[[Category: rhodococcus erythropolis]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 03:37:33 2007''
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Current revision

The Rhodococcus 20S proteasome with unprocessed pro-peptides

PDB ID 1q5r

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