2x5n

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{{Seed}}
 
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[[Image:2x5n.jpg|left|200px]]
 
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==Crystal Structure of the SpRpn10 VWA domain==
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The line below this paragraph, containing "STRUCTURE_2x5n", creates the "Structure Box" on the page.
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<StructureSection load='2x5n' size='340' side='right'caption='[[2x5n]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2x5n]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X5N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2X5N FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_2x5n| PDB=2x5n | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2x5n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x5n OCA], [https://pdbe.org/2x5n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2x5n RCSB], [https://www.ebi.ac.uk/pdbsum/2x5n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2x5n ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RPN10_SCHPO RPN10_SCHPO] Protects ubiquitin chains against dissambly by deubiquitinating enzymes thereby promoting protein degradation.<ref>PMID:10809753</ref> <ref>PMID:12560082</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x5/2x5n_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2x5n ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Schizosaccharomyces pombe Rpn10 (SpRpn10) is a proteasomal ubiquitin (Ub) receptor located within the 19S regulatory particle where it binds to subunits of both the base and lid sub-particles. We have solved the structure of full-length SpRpn10 by determining the crystal structure of the VWA domain and characterising the full-length protein by NMR. We demonstrate that the single Ub interacting motif (UIM) of SpRpn10 forms a 1:1 complex with K48-linked diUb, which it binds selectively over monoUb and K63-linked diUb. We further show that the SpRpn10 UIM binds to SpRpn12, a subunit of the lid sub-particle, with an affinity comparable to Lys48-linked diUb. This is the first observation of a UIM binding other than a Ub fold and suggests that SpRpn12 could modulate the activity of SpRpn10 as a proteasomal Ub receptor.
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===CRYSTAL STRUCTURE OF THE SPRPN10 VWA DOMAIN===
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The structure of RPN10 and its interactions with polyubiquitin chains and the proteasome subunit RPN12.,Riedinger C, Boehringer J, Trempe JF, Lowe ED, Brown NR, Gehring K, Noble ME, Gordon C, Endicott JA J Biol Chem. 2010 Aug 24. PMID:20739285<ref>PMID:20739285</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2x5n" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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2X5N is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X5N OCA].
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*[[Proteasome 3D structures|Proteasome 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Schizosaccharomyces pombe]]
[[Category: Schizosaccharomyces pombe]]
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[[Category: Bohringer, J.]]
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[[Category: Boehringer J]]
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[[Category: Brown, N R.]]
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[[Category: Brown NR]]
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[[Category: Endicott, J A.]]
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[[Category: Endicott JA]]
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[[Category: Gehring, K.]]
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[[Category: Gehring K]]
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[[Category: Gordon, C.]]
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[[Category: Gordon C]]
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[[Category: Lowe, E D.]]
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[[Category: Lowe ED]]
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[[Category: Noble, M E.M.]]
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[[Category: Noble MEM]]
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[[Category: Riedinger, C.]]
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[[Category: Riedinger C]]
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[[Category: Trempe, J F.]]
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[[Category: Trempe J-F]]
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[[Category: Nuclear protein]]
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[[Category: Nucleus]]
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[[Category: Proteasome]]
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[[Category: Ubiquitin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 25 08:50:46 2010''
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Current revision

Crystal Structure of the SpRpn10 VWA domain

PDB ID 2x5n

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