RNA Interference

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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3dlh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dlh OCA], [http://www.ebi.ac.uk/pdbsum/3dlh PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3dlh RCSB]</span>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3dlh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dlh OCA], [http://www.ebi.ac.uk/pdbsum/3dlh PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3dlh RCSB]</span>
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}}
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[[Image:500px-ShRNA Lentivirus.png|350px|left|thumb| Schematic of RNAi Interference in Mammalian Cells]]
 
[[RNA Interference]] (RNAi) is a system in living cells that helps control the level of activity of genes. In RNAi, a long double stranded [[RNA]] is cleaved into shorter strands. One strand known as the guide strand is complimentary to the mRNA gene product of a gene of interest. This guide strand is incorporated into an RNA-induced silencing complex (RISC) and subsequently binds the mRNA of the gene of interest. After binding, the RNA/RISC complex subsequently silences the mRNA of interest via cleavage of the mRNA strand.
[[RNA Interference]] (RNAi) is a system in living cells that helps control the level of activity of genes. In RNAi, a long double stranded [[RNA]] is cleaved into shorter strands. One strand known as the guide strand is complimentary to the mRNA gene product of a gene of interest. This guide strand is incorporated into an RNA-induced silencing complex (RISC) and subsequently binds the mRNA of the gene of interest. After binding, the RNA/RISC complex subsequently silences the mRNA of interest via cleavage of the mRNA strand.
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[[Image:500px-ShRNA Lentivirus.png|350px|left|thumb| Schematic of RNAi Interference in Mammalian Cells]]
{{Structure
{{Structure
|PDB= 1rpu |SIZE=300|ALIGN='left'|SCENE=User:Wayne_Decatur/Plant_Viral_Protein_p19_Suppression_of_RNA_Silencing/View1/2|CAPTION= viral silencing suppressor bound to a siRNA ([[1rpu]]), resolution 2.50&Aring; (<scene name='User:Wayne_Decatur/Plant_Viral_Protein_p19_Suppression_of_RNA_Silencing/View1/2'>initial scene</scene>)
|PDB= 1rpu |SIZE=300|ALIGN='left'|SCENE=User:Wayne_Decatur/Plant_Viral_Protein_p19_Suppression_of_RNA_Silencing/View1/2|CAPTION= viral silencing suppressor bound to a siRNA ([[1rpu]]), resolution 2.50&Aring; (<scene name='User:Wayne_Decatur/Plant_Viral_Protein_p19_Suppression_of_RNA_Silencing/View1/2'>initial scene</scene>)
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**[[Tomato aspermy virus protein 2b Suppression of RNA Silencing|Tomato aspermy virus protein 2b Suppression of RNA Silencing]]
**[[Tomato aspermy virus protein 2b Suppression of RNA Silencing|Tomato aspermy virus protein 2b Suppression of RNA Silencing]]
**[[Flock house virus B2 protein Suppression of RNA Silencing|Flock house virus B2 protein Suppression of RNA Silencing]]
**[[Flock house virus B2 protein Suppression of RNA Silencing|Flock house virus B2 protein Suppression of RNA Silencing]]
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To view automatically seeded indices concerning RNA Interference See:
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See RNAi in action by viewing this
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[http://www.nature.com/nrg/multimedia/rnai/index.html RNAi Animation].
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To view automatically seeded indices concerning RNA Interference, see:
**[[:Category:Argonaute|Argonaute]]
**[[:Category:Argonaute|Argonaute]]
**[[:Category:Dicer|Dicer]]
**[[:Category:Dicer|Dicer]]
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To view other Proteopedia pages about diseases & drug targets, See: [[Pharmaceutical_Drug_Targets|Pharmaceutical Drug Targets]]
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To view other Proteopedia pages about diseases & drug targets, see: [[Pharmaceutical_Drug_Targets|Pharmaceutical Drug Targets]]

Current revision

PDB ID 3dlh

Drag the structure with the mouse to rotate
guide-strand-containing Argonaute protein silencing complex (3dlh), resolution 3.00Å ()
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml


RNA Interference (RNAi) is a system in living cells that helps control the level of activity of genes. In RNAi, a long double stranded RNA is cleaved into shorter strands. One strand known as the guide strand is complimentary to the mRNA gene product of a gene of interest. This guide strand is incorporated into an RNA-induced silencing complex (RISC) and subsequently binds the mRNA of the gene of interest. After binding, the RNA/RISC complex subsequently silences the mRNA of interest via cleavage of the mRNA strand.

Schematic of RNAi Interference in Mammalian Cells
Schematic of RNAi Interference in Mammalian Cells

PDB ID 1rpu

Drag the structure with the mouse to rotate
viral silencing suppressor bound to a siRNA (1rpu), resolution 2.50Å ()
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml


Articles in Proteopedia concerning RNA Interference include:


See RNAi in action by viewing this RNAi Animation.


To view automatically seeded indices concerning RNA Interference, see:


To view other Proteopedia pages about diseases & drug targets, see: Pharmaceutical Drug Targets

Proteopedia Page Contributors and Editors (what is this?)

David Canner, Wayne Decatur, Eric Martz

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