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1ob8

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[[Image:1ob8.gif|left|200px]]<br />
 
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<applet load="1ob8" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ob8, resolution 1.80&Aring;" />
 
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'''HOLLIDAY JUNCTION RESOLVING ENZYME'''<br />
 
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==Overview==
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==Holliday Junction Resolving Enzyme==
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Two archaeal Holliday junction resolving enzymes, Holliday junction, cleavage (Hjc) and Holliday junction endonuclease (Hje), have been, characterized. Both are members of a nuclease superfamily that includes, the type II restriction enzymes, although their DNA cleaving activity is, highly specific for four-way junction structure and not nucleic acid, sequence. Despite 28% sequence identity, Hje and Hjc cleave junctions with, distinct cutting patterns--they cut different strands of a four-way, junction, at different distances from the junction centre. We report the, high-resolution crystal structure of Hje from Sulfolobus solfataricus. The, structure provides a basis to explain the differences in substrate, specificity of Hje and Hjc, which result from changes in dimer, organization, and ... [[http://ispc.weizmann.ac.il/pmbin/getpm?15479781 (full description)]]
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<StructureSection load='1ob8' size='340' side='right'caption='[[1ob8]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ob8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OB8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OB8 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ob8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ob8 OCA], [https://pdbe.org/1ob8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ob8 RCSB], [https://www.ebi.ac.uk/pdbsum/1ob8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ob8 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HJE_SACS2 HJE_SACS2] A structure-specific endonuclease that resolves Holliday junction (HJ) intermediates during genetic recombination. Acts only on 4-way DNA junctions in a sequence non-specific manner; introduces paired nicks in opposing strands 2 bases 3' of the point of strand exchange only on continuous strands of 4-way junction DNA. Cleaves both mobile and immobile junctions.<ref>PMID:10623519</ref> <ref>PMID:10736227</ref> <ref>PMID:15921693</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ob/1ob8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ob8 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1OB8 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]] with SO4 and EDO as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: SA1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OB8 OCA]].
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*[[Resolvase 3D structures|Resolvase 3D structures]]
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== References ==
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==Reference==
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<references/>
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Substrate recognition and catalysis by the Holliday junction resolving enzyme Hje., Middleton CL, Parker JL, Richard DJ, White MF, Bond CS, Nucleic Acids Res. 2004 Oct 12;32(18):5442-51. Print 2004. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15479781 15479781]
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__TOC__
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[[Category: Single protein]]
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</StructureSection>
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[[Category: Sulfolobus solfataricus]]
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[[Category: Large Structures]]
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[[Category: Bond, C.S.]]
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[[Category: Saccharolobus solfataricus]]
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[[Category: Middleton, C.L.]]
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[[Category: Bond CS]]
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[[Category: Parker, J.L.]]
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[[Category: Middleton CL]]
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[[Category: Richard, D.J.]]
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[[Category: Parker JL]]
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[[Category: White, M.F.]]
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[[Category: Richard DJ]]
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[[Category: EDO]]
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[[Category: White MF]]
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[[Category: SO4]]
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[[Category: archaea]]
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[[Category: enzyme]]
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[[Category: holliday junction resolving enzyme]]
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[[Category: homologous recombination]]
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[[Category: hydrolase]]
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[[Category: nuclease]]
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[[Category: thermophile]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:11:45 2007''
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Current revision

Holliday Junction Resolving Enzyme

PDB ID 1ob8

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