3ipm

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[[Image:3ipm.png|left|200px]]
 
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==Crystal Structure of Archaeal 20S Proteasome in Complex with the C-terminus of PAN==
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The line below this paragraph, containing "STRUCTURE_3ipm", creates the "Structure Box" on the page.
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<StructureSection load='3ipm' size='340' side='right'caption='[[3ipm]], [[Resolution|resolution]] 4.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3ipm]] is a 21 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii], [https://en.wikipedia.org/wiki/Thermoplasma_acidophilum Thermoplasma acidophilum] and [https://en.wikipedia.org/wiki/Trypanosoma_brucei Trypanosoma brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IPM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IPM FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ipm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ipm OCA], [https://pdbe.org/3ipm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ipm RCSB], [https://www.ebi.ac.uk/pdbsum/3ipm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ipm ProSAT]</span></td></tr>
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{{STRUCTURE_3ipm| PDB=3ipm | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PSA_THEAC PSA_THEAC] Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation. The T.acidophilum proteasome is able to cleave oligopeptides after Tyr, Leu, Phe, and to a lesser extent after Glu and Arg. Thus, displays chymotrypsin-like activity and low level of caspase-like and trypsin-like activities.<ref>PMID:8999862</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ip/3ipm_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ipm ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Protein degradation in the 20S proteasome is regulated in eukaryotes by the 19S ATPase complex and in archaea by the homologous PAN ATPase ring complex. Subunits of these hexameric ATPases contain on their C-termini a conserved hydrophobic-tyrosine-X (HbYX) motif that docks into pockets in the 20S to stimulate the opening of a gated substrate entry channel. Here, we report the crystal structure of the archaeal 20S proteasome in complex with the C-terminus of the archaeal proteasome regulatory ATPase, PAN. This structure defines the detailed interactions between the critical C-terminal HbYX motif and the 20S alpha-subunits and indicates that the intersubunit pocket in the 20S undergoes an induced-fit conformational change on binding of the HbYX motif. This structure together with related mutagenesis data suggest how in eukaryotes certain proteasomal ATPases bind to specific pockets in an asymmetrical manner to regulate gate opening.
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===Crystal Structure of Archaeal 20S Proteasome in Complex with the C-terminus of PAN===
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Interactions of PAN's C-termini with archaeal 20S proteasome and implications for the eukaryotic proteasome-ATPase interactions.,Yu Y, Smith DM, Kim HM, Rodriguez V, Goldberg AL, Cheng Y EMBO J. 2010 Feb 3;29(3):692-702. Epub 2009 Dec 17. PMID:20019667<ref>PMID:20019667</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The line below this paragraph, {{ABSTRACT_PUBMED_20019667}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3ipm" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20019667 is the PubMed ID number.
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{{ABSTRACT_PUBMED_20019667}}
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==About this Structure==
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[[3ipm]] is a 21 chain structure of [[Proteasome]] with sequence from [http://en.wikipedia.org/wiki/Thermoplasma_acidophilum Thermoplasma acidophilum] and [http://en.wikipedia.org/wiki/Trypanosoma_brucei,_methanocaldococcus_jannaschii Trypanosoma brucei, methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IPM OCA].
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==See Also==
==See Also==
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*[[Proteasome]]
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*[[Proteasome 3D structures|Proteasome 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:20019667</ref><references group="xtra"/>
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__TOC__
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[[Category: Proteasome endopeptidase complex]]
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Methanocaldococcus jannaschii]]
[[Category: Thermoplasma acidophilum]]
[[Category: Thermoplasma acidophilum]]
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[[Category: Trypanosoma brucei, methanocaldococcus jannaschii]]
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[[Category: Trypanosoma brucei]]
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[[Category: Cheng, Y.]]
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[[Category: Cheng Y]]
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[[Category: Yu, Y.]]
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[[Category: Yu Y]]
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[[Category: Aaa atpase]]
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[[Category: Electron cryomicroscopy]]
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[[Category: Hydrolase]]
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[[Category: Hydrolase-hydrolase activator complex]]
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[[Category: Protease]]
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[[Category: Proteasomal atpase]]
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[[Category: Proteasome]]
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[[Category: Protein degradation]]
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[[Category: Threonine protease]]
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Current revision

Crystal Structure of Archaeal 20S Proteasome in Complex with the C-terminus of PAN

PDB ID 3ipm

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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