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Resolvase

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[[Image:1kcf.png|left|200px|thumb|Crystal structure of yeast Holliday junction resolvase, [[1kcf]]]]
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<StructureSection load='1kcf' size='340' side='right' caption='Yeast Holliday junction resolvase dimer complex with sulfate, [[1kcf]]' scene='' >
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{{STRUCTURE_1kcf| PDB=1kcf | SIZE=300| SCENE=Resolvase/Cv/1 |right|CAPTION=Yeast Holliday junction resolvase, [[1kcf]] }}
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== Function ==
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[[Resolvase]] or '''recombinase''' (Rec) is a nuclease which is involved in DNA recombination. The Holliday junction resolvase (HJR) resolves 4-way DNA intermediates known as Holliday junctions. Recombination of 2 DNA sites occurs when recombinase binds to the 2 strands. According to the binding residue, the recombinases are grouped to Tyr- and Ser-recombinase. Tyr-recombinases include Cre, FLP and lambda integrase (LamInt). Ser-recombinases (S-rec) include gamma-delta resolvase (GDR), Tn3 resolvase and phiC31 integrase. RadA recombinase promotes DNA recombination. The images at the left and at the right correspond to one representative resolvase structure, ''i.e.'' the crystal structure of Ydc2 (also known as SpCce1), a Holliday junction resolvase from the fission yeast ''Schizosaccharomyces pombe'' that is involved in the maintenance of mitochondrial DNA ([[1kcf]]). Two sulfate ions are shown as space filling objects. Detailed analysis of its <scene name='Resolvase/Cv/2'>dimeric structure</scene> suggests mechanisms for junction isomerization and communication between the two active sites <ref>PMID:11726496</ref>.
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[[Resolvase]] or '''recombinase''' (Rec) is a nuclease which is involved in DNA recombination. According to the binding residue, the recombinases are grouped to Tyr- and Ser-recombinase.
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{{TOC limit|limit=2}}
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*'''Holliday junction resolvase''' (HJR) resolves 4-way DNA intermediates known as Holliday junctions. Recombination of 2 DNA sites occurs when recombinase binds to the 2 strands<ref>PMID:19442245</ref>. <br />
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*'''Tyr-recombinases''' include Cre which recombines loxP sites, FLP and lambda integrase (LamInt). <br />
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*'''Ser-recombinases''' (S-rec) include gamma-delta resolvase (GDR), Tn3 resolvase and phiC31 integrase.<br />
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*'''RadA recombinase''' promotes DNA recombination. Detailed analysis of its <scene name='Resolvase/Cv/2'>dimeric structure</scene> suggests mechanisms for junction isomerization and communication between the two active sites <ref>PMID:19143611</ref>. <br />
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*'''Hin-recombinase''' (HRec) is a protein of ''Salmonella''. HRec inverts a 900 base pair DNA segment which contains the promoters of flagellar genes. The inversion changes the these genes' expressions<ref>PMID:15454079</ref>.
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== Holliday junction resolvase ==
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==3D structures of resolvase==
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[[Resolvase 3D structures]]
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</StructureSection>
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[[2wiw]], [[2wiz]], [[2wj0]] – AfHJR+DNA – ''Archaeoglobus fulgidus''<br />
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[[2wcw]], [[2wcz]] – AfHJR (mutant)<br />
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[[2h8c]] – EcHJR (mutant) +DNA – ''Escherichia coli''<br />
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[[1gdt]] - EcHJR +DNA<br />
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[[2h8e]] - EcHJR (mutant)<br />
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[[1q8r]], [[1hjr]] – EcHJR<br />
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[[1hjr]] – EcHJR RuvC<br />
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[[1zp7]] – HJR – ''Bacillus subtilis''<br />
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[[1ob8]], [[1ob9]], [[1hh1]] – SsHJR – ''Sulfolobus solfataricus''<br />
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[[2fco]] – HJR – ''Geobacillus kaustophilus''<br />
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[[1ipi]], [[1gef]] – HJR – ''Pyrococcus furiosus''<br />
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[[1kcf]] – HJR – fission yeast<br />
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== Ser-recombinase ==
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[[2rsl]] - EcGDR<br />
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[[1hx7]] – EcGDR N-terminal – NMR<br />
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[[1ght]], [[1res]], [[1ret]], [[1ght]] – EcGDR catalytic domain – NMR<br />
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[[1gdr]], [[1hx7]] - EcGDR catalytic domain<br />
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[[2gm4]], [[1zr2]], [[1zr4]], [[1gdt]] – EcGDR+DNA<br />
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[[2gm5]] – EcGDR (mutant)<br />
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[[3guv]] – site-specific recombinase – ''Streptococcus pneumoniae''<br />
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[[2r0q]] – S-rec+DNA – ''Staphylococcus aureus''<br />
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[[3lhf]] – SsS-rec<br />
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== Tyr-recombinase ==
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[[3mgv]], [[3c28]], [[3c29]], [[2hof]], [[2hoi]], [[1xns]], [[1xo0]], [[1pvp]], [[1pvq]], [[1pvr]], [[1nzb]], [[1ouq]], [[1q3u]], [[1q3v]], [[1ma7]], [[1kbu]], [[1drg]], [[1f44]], [[2crx]], [[3crx]], [[4crx]], [[5crx]], [[1crx]] – EpCre+DNA – Enterobacteria phage PI<br />
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[[3etl]], [[3ew9]], [[3ewa]], [[2i1q]] – MmRadA Rec+AMPPNP+ion – ''Methanococcus maripaludis''<br />
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[[2b21]] - MmRadA Rec+AMPPNP<br />
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[[2zub]], [[2zuc]], [[2zud]], [[2dfl]] – SsRadA<br />
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[[2cvf]], [[2cvh]] – RadB Rec – ''Thermococcus kodakarensis''<br />
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[[2f1h]], [[2fpl]], [[2fpm]] - MvRec+AMPPNP+K – ''Methanococcus voltae''<br />
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[[2f1i]] – MvRec+AMPPNP<br />
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[[2f1j]], [[2fpk]] - MvRec+ADP<br />
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[[1a0p]] – EcXerd Rec<br />
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== Integrase ==
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[[2a3v]] – Rec INTI4+DNA – ''Vibrio cholerae''<br />
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[[3bvp]] – Integrase N-terminal – Lactococcus phage<br />
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[[2oxo]] – LamInt binding domain – phage<br />
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[[1z19]] – EpLamInt core binding +catalytic domain+DNA<br />
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[[1z1b]], [[1z1g]], [[1p7d]] – EpLamInt+DNA<br />
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[[1kjk]] – EpLamInt N-terminal – NMR<br />
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[[1ae9]] – EpLamInt catalytic core<br />
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[[1p4e]], [[1m6x]] – yFLP (mutant)+DNA – yeast<br />
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[[1flo]] - yFLP +DNA<br />
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[[1aih]] – HP1 integrase catalytic domain - bacteriophage<br />
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==References==
==References==

Current revision

Yeast Holliday junction resolvase dimer complex with sulfate, 1kcf

Drag the structure with the mouse to rotate

References

  1. ↑ West SC. The search for a human Holliday junction resolvase. Biochem Soc Trans. 2009 Jun;37(Pt 3):519-26. doi: 10.1042/BST0370519. PMID:19442245 doi:http://dx.doi.org/10.1042/BST0370519
  2. ↑ Haldenby S, White MF, Allers T. RecA family proteins in archaea: RadA and its cousins. Biochem Soc Trans. 2009 Feb;37(Pt 1):102-7. doi: 10.1042/BST0370102. PMID:19143611 doi:http://dx.doi.org/10.1042/BST0370102
  3. ↑ Dhar G, Sanders ER, Johnson RC. Architecture of the hin synaptic complex during recombination: the recombinase subunits translocate with the DNA strands. Cell. 2004 Oct 1;119(1):33-45. PMID:15454079 doi:http://dx.doi.org/10.1016/j.cell.2004.09.010

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