|
|
| (4 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| - | [[Image:3tuf.png|left|200px]] | |
| | | | |
| - | {{STRUCTURE_3tuf| PDB=3tuf | SCENE= }}
| + | ==Structure of the SpoIIQ-SpoIIIAH pore forming complex.== |
| - | | + | <StructureSection load='3tuf' size='340' side='right'caption='[[3tuf]], [[Resolution|resolution]] 2.26Å' scene=''> |
| - | ===Structure of the SpoIIQ-SpoIIIAH pore forming complex.===
| + | == Structural highlights == |
| - | | + | <table><tr><td colspan='2'>[[3tuf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TUF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TUF FirstGlance]. <br> |
| - | {{ABSTRACT_PUBMED_22431604}}
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.26Å</td></tr> |
| - | | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tuf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tuf OCA], [https://pdbe.org/3tuf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tuf RCSB], [https://www.ebi.ac.uk/pdbsum/3tuf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tuf ProSAT]</span></td></tr> |
| - | ==About this Structure== | + | </table> |
| - | [[3tuf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TUF OCA]. | + | == Function == |
| - | | + | [https://www.uniprot.org/uniprot/SP2Q_BACSU SP2Q_BACSU] Involved in forespore engulfment and required for anchoring membrane proteins on the forespore side of the septal membrane. Forms a channel with SpoIIIAH that is open on the forespore end and closed (or gated) on the mother cell end. This allows sigma-E-directed gene expression in the mother-cell compartment of the sporangium to trigger the activation of sigma-G forespore-specific gene expression by a pathway of intercellular signaling.<ref>PMID:9140963</ref> <ref>PMID:10781563</ref> <ref>PMID:15882622</ref> <ref>PMID:15752199</ref> <ref>PMID:16164552</ref> <ref>PMID:16959571</ref> <ref>PMID:17824930</ref> <ref>PMID:18077456</ref> <ref>PMID:18485064</ref> <ref>PMID:18812514</ref> |
| - | ==Reference== | + | == References == |
| - | <ref group="xtra">PMID:022431604</ref><references group="xtra"/> | + | <references/> |
| | + | __TOC__ |
| | + | </StructureSection> |
| | [[Category: Bacillus subtilis]] | | [[Category: Bacillus subtilis]] |
| - | [[Category: Blagova, E V.]] | + | [[Category: Large Structures]] |
| - | [[Category: Levdikov, V M.]] | + | [[Category: Blagova EV]] |
| - | [[Category: Wilkinson, A J.]] | + | [[Category: Levdikov VM]] |
| - | [[Category: Cell engulfment and signalling]] | + | [[Category: Wilkinson AJ]] |
| - | [[Category: Intercellular channel]]
| + | |
| - | [[Category: Intercellular signalling]]
| + | |
| - | [[Category: Intercellular space]]
| + | |
| - | [[Category: Signaling protein]]
| + | |
| - | [[Category: Sporulation]]
| + | |
| Structural highlights
Function
SP2Q_BACSU Involved in forespore engulfment and required for anchoring membrane proteins on the forespore side of the septal membrane. Forms a channel with SpoIIIAH that is open on the forespore end and closed (or gated) on the mother cell end. This allows sigma-E-directed gene expression in the mother-cell compartment of the sporangium to trigger the activation of sigma-G forespore-specific gene expression by a pathway of intercellular signaling.[1] [2] [3] [4] [5] [6] [7] [8] [9] [10]
References
- ↑ Londono-Vallejo JA, Frehel C, Stragier P. SpoIIQ, a forespore-expressed gene required for engulfment in Bacillus subtilis. Mol Microbiol. 1997 Apr;24(1):29-39. PMID:9140963
- ↑ Sun YL, Sharp MD, Pogliano K. A dispensable role for forespore-specific gene expression in engulfment of the forespore during sporulation of Bacillus subtilis. J Bacteriol. 2000 May;182(10):2919-27. PMID:10781563
- ↑ Dworkin J, Losick R. Developmental commitment in a bacterium. Cell. 2005 May 6;121(3):401-9. PMID:15882622 doi:http://dx.doi.org/10.1016/j.cell.2005.02.032
- ↑ Doan T, Marquis KA, Rudner DZ. Subcellular localization of a sporulation membrane protein is achieved through a network of interactions along and across the septum. Mol Microbiol. 2005 Mar;55(6):1767-81. PMID:15752199 doi:http://dx.doi.org/10.1111/j.1365-2958.2005.04501.x
- ↑ Jiang X, Rubio A, Chiba S, Pogliano K. Engulfment-regulated proteolysis of SpoIIQ: evidence that dual checkpoints control sigma activity. Mol Microbiol. 2005 Oct;58(1):102-15. PMID:16164552 doi:http://dx.doi.org/MMI4811
- ↑ Broder DH, Pogliano K. Forespore engulfment mediated by a ratchet-like mechanism. Cell. 2006 Sep 8;126(5):917-28. PMID:16959571 doi:http://dx.doi.org/10.1016/j.cell.2006.06.053
- ↑ Aung S, Shum J, Abanes-De Mello A, Broder DH, Fredlund-Gutierrez J, Chiba S, Pogliano K. Dual localization pathways for the engulfment proteins during Bacillus subtilis sporulation. Mol Microbiol. 2007 Sep;65(6):1534-46. PMID:17824930 doi:http://dx.doi.org/MMI5887
- ↑ Campo N, Marquis KA, Rudner DZ. SpoIIQ anchors membrane proteins on both sides of the sporulation septum in Bacillus subtilis. J Biol Chem. 2008 Feb 22;283(8):4975-82. Epub 2007 Dec 11. PMID:18077456 doi:http://dx.doi.org/10.1074/jbc.M708024200
- ↑ Camp AH, Losick R. A novel pathway of intercellular signalling in Bacillus subtilis involves a protein with similarity to a component of type III secretion channels. Mol Microbiol. 2008 Jul;69(2):402-17. doi: 10.1111/j.1365-2958.2008.06289.x. PMID:18485064 doi:http://dx.doi.org/10.1111/j.1365-2958.2008.06289.x
- ↑ Meisner J, Wang X, Serrano M, Henriques AO, Moran CP Jr. A channel connecting the mother cell and forespore during bacterial endospore formation. Proc Natl Acad Sci U S A. 2008 Sep 30;105(39):15100-5. doi:, 10.1073/pnas.0806301105. Epub 2008 Sep 23. PMID:18812514 doi:http://dx.doi.org/10.1073/pnas.0806301105
|