2x5n

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[[Image:2x5n.png|left|200px]]
 
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{{STRUCTURE_2x5n| PDB=2x5n | SCENE= }}
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==Crystal Structure of the SpRpn10 VWA domain==
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<StructureSection load='2x5n' size='340' side='right'caption='[[2x5n]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2x5n]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X5N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2X5N FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2x5n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x5n OCA], [https://pdbe.org/2x5n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2x5n RCSB], [https://www.ebi.ac.uk/pdbsum/2x5n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2x5n ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RPN10_SCHPO RPN10_SCHPO] Protects ubiquitin chains against dissambly by deubiquitinating enzymes thereby promoting protein degradation.<ref>PMID:10809753</ref> <ref>PMID:12560082</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x5/2x5n_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2x5n ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Schizosaccharomyces pombe Rpn10 (SpRpn10) is a proteasomal ubiquitin (Ub) receptor located within the 19S regulatory particle where it binds to subunits of both the base and lid sub-particles. We have solved the structure of full-length SpRpn10 by determining the crystal structure of the VWA domain and characterising the full-length protein by NMR. We demonstrate that the single Ub interacting motif (UIM) of SpRpn10 forms a 1:1 complex with K48-linked diUb, which it binds selectively over monoUb and K63-linked diUb. We further show that the SpRpn10 UIM binds to SpRpn12, a subunit of the lid sub-particle, with an affinity comparable to Lys48-linked diUb. This is the first observation of a UIM binding other than a Ub fold and suggests that SpRpn12 could modulate the activity of SpRpn10 as a proteasomal Ub receptor.
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===CRYSTAL STRUCTURE OF THE SPRPN10 VWA DOMAIN===
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The structure of RPN10 and its interactions with polyubiquitin chains and the proteasome subunit RPN12.,Riedinger C, Boehringer J, Trempe JF, Lowe ED, Brown NR, Gehring K, Noble ME, Gordon C, Endicott JA J Biol Chem. 2010 Aug 24. PMID:20739285<ref>PMID:20739285</ref>
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{{ABSTRACT_PUBMED_20739285}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2x5n" style="background-color:#fffaf0;"></div>
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[[2x5n]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X5N OCA].
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==See Also==
==See Also==
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*[[Proteasome|Proteasome]]
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*[[Proteasome 3D structures|Proteasome 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:020739285</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Schizosaccharomyces pombe]]
[[Category: Schizosaccharomyces pombe]]
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[[Category: Boehringer, J.]]
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[[Category: Boehringer J]]
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[[Category: Brown, N R.]]
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[[Category: Brown NR]]
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[[Category: Endicott, J A.]]
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[[Category: Endicott JA]]
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[[Category: Gehring, K.]]
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[[Category: Gehring K]]
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[[Category: Gordon, C.]]
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[[Category: Gordon C]]
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[[Category: Lowe, E D.]]
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[[Category: Lowe ED]]
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[[Category: Noble, M E.M.]]
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[[Category: Noble MEM]]
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[[Category: Riedinger, C.]]
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[[Category: Riedinger C]]
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[[Category: Trempe, J F.]]
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[[Category: Trempe J-F]]
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[[Category: Nuclear protein]]
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[[Category: Nucleus]]
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[[Category: Proteasome]]
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[[Category: Ubiquitin]]
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Current revision

Crystal Structure of the SpRpn10 VWA domain

PDB ID 2x5n

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