2vyi

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (05:33, 17 October 2024) (edit) (undo)
 
(8 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2vyi.png|left|200px]]
 
-
{{STRUCTURE_2vyi| PDB=2vyi | SCENE= }}
+
==Crystal Structure of the TPR domain of Human SGT==
 +
<StructureSection load='2vyi' size='340' side='right'caption='[[2vyi]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2vyi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VYI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VYI FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vyi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vyi OCA], [https://pdbe.org/2vyi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vyi RCSB], [https://www.ebi.ac.uk/pdbsum/2vyi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vyi ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/SGTA_HUMAN SGTA_HUMAN] Co-chaperone that binds directly to HSC70 and HSP70 and regulates their ATPase activity.<ref>PMID:18759457</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vy/2vyi_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vyi ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The small glutamine-rich tetratricopeptide repeat protein (SGT) belongs to a family of cochaperones that interacts with both Hsp70 and Hsp90 via the so-called TPR domain. Here, we present the crystal structure of the TPR domain of human SGT (SGT-TPR), which shows that it contains typical features found in the structures of other TPR domains. Previous studies show that full-length SGT can bind to both Vpu and Gag of human immunodeficiency virus type 1 (HIV-1) and the overexpression of SGT in cells reduces the efficiency of HIV-1 particle release. We show that SGT-TPR can bind Vpu and reduce the amount of HIV-1 p24, which is the viral capsid, secreted from cells transfected with the HIV-1 proviral construct, albeit at a lower efficiency than full-length SGT. This indicates that the TPR domain of SGT is sufficient for the inhibition of HIV-1 particle release but the N- and/or C-terminus also have some contributions. The SGT binding site in Vpu was also identified by using peptide array and confirmed by GST pull-down assay.
-
===CRYSTAL STRUCTURE OF THE TPR DOMAIN OF HUMAN SGT===
+
Structural and functional characterization of human SGT and its interaction with Vpu of the human immunodeficiency virus type 1.,Dutta S, Tan YJ Biochemistry. 2008 Sep 23;47(38):10123-31. Epub 2008 Aug 30. PMID:18759457<ref>PMID:18759457</ref>
-
{{ABSTRACT_PUBMED_18759457}}
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 2vyi" style="background-color:#fffaf0;"></div>
-
==About this Structure==
+
==See Also==
-
[[2vyi]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VYI OCA].
+
*[[Small glutamine-rich tetratricopeptide repeat containing protein alpha|Small glutamine-rich tetratricopeptide repeat containing protein alpha]]
-
 
+
== References ==
-
==Reference==
+
<references/>
-
<ref group="xtra">PMID:018759457</ref><references group="xtra"/>
+
__TOC__
 +
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Dutta, S.]]
+
[[Category: Large Structures]]
-
[[Category: Tan, Y J.]]
+
[[Category: Dutta S]]
-
[[Category: Chaperone]]
+
[[Category: Tan YJ]]
-
[[Category: Host-virus interaction]]
+
-
[[Category: Phosphoprotein]]
+
-
[[Category: Sgt]]
+
-
[[Category: Tetratricopeptide repeat protein]]
+
-
[[Category: Tpr repeat]]
+

Current revision

Crystal Structure of the TPR domain of Human SGT

PDB ID 2vyi

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2vyi"
Personal tools