2ku2

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{{Large structure}}
 
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{{STRUCTURE_2ku2| PDB=2ku2 | SCENE= }}
 
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===Dynamic Regulation of Archaeal Proteasome Gate Opening as Studied by TROSY-NMR===
 
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{{ABSTRACT_PUBMED_20360109}}
 
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==About this Structure==
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==Dynamic Regulation of Archaeal Proteasome Gate Opening as Studied by TROSY-NMR==
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[[2ku2]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermoplasma_acidophilum Thermoplasma acidophilum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KU2 OCA].
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<StructureSection load='2ku2' size='340' side='right'caption='[[2ku2]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2ku2]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoplasma_acidophilum Thermoplasma acidophilum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KU2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KU2 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ku2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ku2 OCA], [https://pdbe.org/2ku2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ku2 RCSB], [https://www.ebi.ac.uk/pdbsum/2ku2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ku2 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PSA_THEAC PSA_THEAC] Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation. The T.acidophilum proteasome is able to cleave oligopeptides after Tyr, Leu, Phe, and to a lesser extent after Glu and Arg. Thus, displays chymotrypsin-like activity and low level of caspase-like and trypsin-like activities.<ref>PMID:8999862</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ku/2ku2_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ku2 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The proteasome catalyzes the majority of protein degradation in the cell and plays an integral role in cellular homeostasis. Control over proteolysis by the 20S core-particle (CP) proteasome is achieved by gated access of substrate; thus, an understanding of the molecular mechanism by which these gates regulate substrate entry is critical. We used methyl-transverse relaxation optimized nuclear magnetic resonance spectroscopy to show that the amino-terminal residues that compose the gates of the alpha subunits of the Thermoplasma acidophilum proteasome are highly dynamic over a broad spectrum of time scales and that gating termini are in conformations that extend either well inside (closed gate) or outside (open gate) of the antechamber. Interconversion between these conformers on a time scale of seconds leads to a dynamic regulation of 20S CP proteolysis activity.
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==See Also==
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Dynamic regulation of archaeal proteasome gate opening as studied by TROSY NMR.,Religa TL, Sprangers R, Kay LE Science. 2010 Apr 2;328(5974):98-102. PMID:20360109<ref>PMID:20360109</ref>
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*[[Jmol/Visualizing large molecules|Jmol/Visualizing large molecules]]
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*[[Proteasome|Proteasome]]
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:020360109</ref><references group="xtra"/>
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</div>
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[[Category: Proteasome endopeptidase complex]]
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<div class="pdbe-citations 2ku2" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Proteasome 3D structures|Proteasome 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Thermoplasma acidophilum]]
[[Category: Thermoplasma acidophilum]]
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[[Category: Kay, L E.]]
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[[Category: Kay LE]]
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[[Category: Religa, T L.]]
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[[Category: Religa TL]]
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[[Category: Sprangers, R.]]
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[[Category: Sprangers R]]
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[[Category: Gating residue]]
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[[Category: Hydrolase]]
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[[Category: Protease]]
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[[Category: Proteasome]]
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[[Category: Proteolysis]]
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[[Category: Threonine protease]]
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Current revision

Dynamic Regulation of Archaeal Proteasome Gate Opening as Studied by TROSY-NMR

PDB ID 2ku2

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