Rhomboid protease

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(New page: <StructureSection load='4h1d' size='350' side='right' caption='Structure of rhomboid protease complex with inhibitor DFP (PDB entry 4h1d)' scene=''> '''Rhomboid protease''' is a tran...)
Current revision (08:59, 8 August 2024) (edit) (undo)
 
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<StructureSection load='' size='450' side='right' caption='Structure of rhomboid protease complex with inhibitor isocoumarin and nonyglucoside detergent molecules (PDB entry [[2xow]])' scene='55/551211/Cv/1'>
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== Function ==
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'''Rhomboid protease''' ('''GlpG''') is a transmembranal serine protease. The active site is buried in the lipid bilayer of cell membrane. It cleaves proteins within their transmembrane domain<ref>PMID:18605900</ref>. Rhomboids regulate many cellular processes and are involved in many human diseases. The catalytic site of rhomboids contains a hydrophilic pocket protected from the lipid bilayer. Rhomboids are specific for a single transmembranal helix proteins.
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*'''Rhomboid protease YqgP''' interacts with the membrane bound metlloprotease FisH and cleaves the high-affinity Mg transporter<ref>PMID:31930742</ref>.
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<StructureSection load='4h1d' size='350' side='right' caption='Structure of rhomboid protease complex with inhibitor DFP (PDB entry [[4h1d]])' scene=''>
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== Structural highlights ==
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The inhibitor <scene name='55/551211/Cv/5'>isocoumarin covalently binds to the GlpG catalytic residues Ser and His</scene> (in deepskyblue)<ref>PMID:20890268</ref>. <scene name='55/551211/Cv/6'>Whole active site</scene>. Water molecules shown as red spheres.
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'''Rhomboid protease''' is a transmembranal serine protease. The active site is buried in the lipid bilayer of cell membrane. It cleaves proteins within their transmembrane domain. Rhomboids regulate many cellular processes and are involved in many human diseases. The catalytic site of rhomboids contains a hydrophilic pocket protected by from the lipid bilayer. Rhomboids are specific for a single transmembranal helix proteins.
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</StructureSection>
== 3D Structures of rhomboid protease ==
== 3D Structures of rhomboid protease ==
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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{{#tree:id=OrganizedByTopic|openlevels=0|
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*Rhomboid protease GlpG
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**[[2ic8]], [[2irv]], [[2nrf]], [[2o7l]], [[3b45]], [[2xov]], [[4njn]], [[4njp]] – EcGlpG core region – ''Escherichia coli '' <BR />
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**[[2xtu]], [[3b44]], [[2xtv]], [[5f5d]] – EcGlpG core region (mutant) <BR />
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**[[4hdd]] – EcGlpG N terminal <BR />
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**[[2lep]], [[2mja]] – EcGlpG N terminal - NMR <BR />
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**[[3odj]] – HiGlpG - ''Haemophilus influenzae '' <BR />
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**[[2nr9]] – HiGlpG (mutant) + detergent <BR />
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**[[2gqc]] – RP N terminal – NMR – ''Pseudomonas aeruginosa''<br />
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*Rhomboid protease GlpG complex with inhibitor
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**[[2xow]], [[3zeb]] – EcGlpG core region + isocoumarin inhibitor <BR />
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**[[3txt]], [[4h1d]] – EcGlpG core region + DFP inhibitor <BR />
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**[[3ubb]] – EcGlpG core region + phosphonofluoridate inhibitor <BR />
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**[[3zmh]], [[3zmi]], [[3zmj]], [[3zot]] – EcGlpG core region + β-lactam inhibitor <BR />
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**[[6pj5]], [[6pj7]], [[6pj8]], [[6pj9]], [[6pja]], [[6pjp]], [[6pjq]], [[6pjr]], [[6pju]] – EcGlpG core region + peptide aldehyde inhibitor <BR />
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**[[5f5j]], [[5f5k]], [[6pj4]] – EcGlpG core region (mutant) + peptide aldehyde inhibitor <BR />
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**[[4qo0]], [[4qo2]], [[5f5g]], [[5f5b]], [[5mtf]], [[5mt6]], [[5mt7]], [[5mt8]], [[6vj8]], [[6jv9]], [[6vjp]], [[6xro]], [[6xrp]] – EcGlpG core region + peptide inhibitor <BR />
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**[[4qnz]] – EcGlpG core region (mutant) + peptide inhibitor <BR />
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===GlpG===
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*Rhomboid protease YqgP
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[[2ic8]], [[2irv]], [[2nrf]], [[2o7l]], [[3b45]], [[2xov]] – EcGlpG core region – ''Escherichia coli '' <BR />
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**[[6r0j]] – YqpG – ''Bacillus subtilis'' - NMR <BR />
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[[2xtu]], [[3b44]], [[2xtv]] – EcGlpG core region (mutant) <BR />
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[[4hdd]] – EcGlpG N terminal <BR />
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[[2lep]] – EcGlpG N terminal - NMR <BR />
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[[2nr9]] – HiGlpG - ''Haemophilus influenzae '' <BR />
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[[2nr9]] – HiGlpG (mutant) + detergent <BR />
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===GlgG complex with inhibitor===
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*Rhomboid protease GluP
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[[2xov]], [[3zeb]] – EcGlpG core region + isocoumarin inhibitor <BR />
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**[[7tj4]] – GluP + LytH – ''Staphylococcus aureus'' <BR />
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[[3txt]], [[4h1d]] – EcGlpG core region + DFP inhibitor <BR />
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}}
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[[3ubb]] – EcGlpG core region + phosphonofluoridate inhibitor <BR />
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== References ==
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[[3zmh]], [[3zmi]], [[3zmhj], [[3zot]] – EcGlpG core region + β-lactam inhibitor <BR />
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<references/>
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[[Category:Topic Page]]

Current revision

Structure of rhomboid protease complex with inhibitor isocoumarin and nonyglucoside detergent molecules (PDB entry 2xow)

Drag the structure with the mouse to rotate

3D Structures of rhomboid protease

Updated on 08-August-2024

References

  1. ↑ Freeman M. Rhomboid proteases and their biological functions. Annu Rev Genet. 2008;42:191-210. doi: 10.1146/annurev.genet.42.110807.091628. PMID:18605900 doi:http://dx.doi.org/10.1146/annurev.genet.42.110807.091628
  2. ↑ Began J, Cordier B, Brezinova J, Delisle J, Hexnerova R, Srb P, Rampirova P, Kozisek M, Baudet M, Coute Y, Galinier A, Veverka V, Doan T, Strisovsky K. Rhomboid intramembrane protease YqgP licenses bacterial membrane protein quality control as adaptor of FtsH AAA protease. EMBO J. 2020 Jan 13:e102935. doi: 10.15252/embj.2019102935. PMID:31930742 doi:http://dx.doi.org/10.15252/embj.2019102935
  3. ↑ Vinothkumar KR, Strisovsky K, Andreeva A, Christova Y, Verhelst S, Freeman M. The structural basis for catalysis and substrate specificity of a rhomboid protease. EMBO J. 2010 Oct 1. PMID:20890268 doi:10.1038/emboj.2010.243

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Michal Harel, Alexander Berchansky, Joel L. Sussman

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