Deoxycytidine kinase

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<StructureSection load='1p61' size='350' side='right' caption='Mouse deoxycytidine kinase complex with ADP and deoxycytidine (PDB entry [[1p61]])' scene='54/543346/Cv/1'>
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[[Image:1p62.png|left|200px|thumb|Crystal structure of human deoxycytidine kinase complex with ADP and chemotherapy agent [[1p62]]]]
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== Function ==
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{{STRUCTURE_1p62| PDB=1p62 | SIZE=350| SCENE= |right|CAPTION= Human deoxycytidine kinase complex with ADP and chemotherapy agent [[1p62]] }}
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'''Deoxycytidine kinase''' (DCK) is required for the phosphorylation of deoxyribonucleosides dC, dG and dA (but not dT and dU) and nucleosides analogs which are used as antiviral and chemotherapeutic agents. <ref>PMID:20614893</ref>
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'''Deoxyadenosine/deoxycytidine kinase''' (DACK) substrates ar cytidine, adenosine, arabinosylcytosine and arabinosyladenine <ref>PMID:6251049</ref>.
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== Relevance ==
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Deficiency of DCK causes resistance to antiviral and chemotherapeutic agents.
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== Structural highlights ==
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The ability of <scene name='54/543346/Cv/4'>DCK to phosphorylate multiple nucleosides arises from the different conformations which the active site glutamic acid can assume</scene> (active site glutamates are in yellow).<ref>PMID:12808445</ref>
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'''Deoxycytidine kinase''' (DCK) is required for the phosphorylation of deoxyribonucleosides dC, dG and dA and nucleosides analogs which are used as antiviral and chemotherapeutic agents. Deficiency of DCK causes resistance to these agents.
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== 3D Structures of deoxycytidine kinase ==
== 3D Structures of deoxycytidine kinase ==
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[[Deoxycytidine kinase 3D structures]]
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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</StructureSection>
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===dCK complex with chemotherapy agent===
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[[1p5z]], [[1p62]], [[2a7q]] – hDCK + ADP + chemotherapy agent – human<br />
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[[2no9]], [[2noa]], [[2no0]], [[2no6]], [[2zi9]] – hDCK (mutant) + ADP + chemotherapy agent <br />
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[[2zia]] – hDCK (mutant) + UDP + chemotherapy agent <br />
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===dCK complex with nucleoside===
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[[1p60]], [[1p61]], [[3kfx]] – hDCK + ADP + dC<br />
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[[2no1]], [[2no7]] – hDCK (mutant) + ADP + dC<br />
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[[2zi3]], [[2zi4]] – hDCK (mutant) + ADP + dA<br />
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[[3hp1]] – hDCK (mutant) + ADP + dT<br />
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[[2zi5]], [[2zi6]] – hDCK (mutant) + UDP + dA<br />
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[[2zi7]] – hDCK (mutant) + UDP + dG<br />
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[[2a2z]], [[3qen]] – hDCK (mutant) + UDP + dC<br />
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[[3qeo]] – hDCK (mutant) + UDP + dT<br />
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[[2a30]] – hDCK (mutant) + dC<br />
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===Other dCK complexes===
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[[2qrn]] – hDCK + UDP + CMP<br />
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== References ==
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[[2qro]] – hDCK + UDP + AMP<br />
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<references/>
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[[3ipx]] – hDCK + ADP + inbhibitor<br />
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[[Category:Topic Page]]
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[[3ipy]] – hDCK + inbhibitor<br />
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[[3qej]] – hDCK (mutant) + UDP <br />
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[[3mjr]] – hDCK + UDP + hydroxyethoxymethylguanine<br />
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Current revision

Mouse deoxycytidine kinase complex with ADP and deoxycytidine (PDB entry 1p61)

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References

  1. ↑ Hazra S, Ort S, Konrad M, Lavie A. Structural and kinetic characterization of human deoxycytidine kinase variants able to phosphorylate 5-substituted deoxycytidine and thymidine analogues . Biochemistry. 2010 Aug 10;49(31):6784-90. PMID:20614893 doi:10.1021/bi100839e
  2. ↑ Møllgaard H. Deoxyadenosine/deoxycytidine kinase from Bacillus subtilis. Purification, characterization, and physiological function. J Biol Chem. 1980 Sep 10;255(17):8216-20 PMID:6251049
  3. ↑ Sabini E, Ort S, Monnerjahn C, Konrad M, Lavie A. Structure of human dCK suggests strategies to improve anticancer and antiviral therapy. Nat Struct Biol. 2003 Jul;10(7):513-9. PMID:12808445 doi:http://dx.doi.org/10.1038/nsb942

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

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