4q1s
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 4q1s is ON HOLD Authors: Beck, P., Heinemeyer, W., Spaeth, A., Elnakady, Y., Mueller, R., Groll, M. Description: Yeast 20S proteasome in Complex wi...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Yeast 20S proteasome in Complex with Kendomycin== | |
| + | <StructureSection load='4q1s' size='340' side='right'caption='[[4q1s]], [[Resolution|resolution]] 2.60Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4q1s]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q1S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Q1S FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2YD:(5R,6R,7S,8R,9R,12S,13E,16S,18S,19R,20AR)-4,7,19-TRIHYDROXY-2,6,8,12,14,16,18-HEPTAMETHYL-6,7,8,9,10,11,12,15,16,17,18,19,20,20A-TETRADECAHYDRO-1,19 5,9-DIEPOXYBENZO[18]ANNULEN-3(5H)-ONE'>2YD</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4q1s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q1s OCA], [https://pdbe.org/4q1s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4q1s RCSB], [https://www.ebi.ac.uk/pdbsum/4q1s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4q1s ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/PSB6_YEAST PSB6_YEAST] The proteasome degrades poly-ubiquitinated proteins in the cytoplasm and in the nucleus. It is essential for the regulated turnover of proteins and for the removal of misfolded proteins. The proteasome is a multicatalytic proteinase complex that is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. It has an ATP-dependent proteolytic activity. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Natural products are a valuable source for novel lead structures in drug discovery, but for the majority of isolated bioactive compounds the cellular targets are unknown. The structurally unique ansa-polyketide kendomycin was reported to exert its potent cytotoxic effects via impairment of the ubiquitin proteasome system, but the exact mode of action remained unclear. Here, we present a systematic biochemical characterization of kendomycin-proteasome interactions in vitro as well as in vivo, including complex structures of wildtype and mutant yeast 20S proteasome with kendomycin. Our results provide evidence for a polypharmacological mode of action for kendomycin's cytotoxic effect on cancer cells. | ||
| - | + | Structural and biochemical characterization of the natural product kendomycin and the 20S proteasome.,Beck P, Heinemeyer W, Spath AL, Elnakady Y, Muller R, Groll M J Mol Biol. 2014 Jul 16. pii: S0022-2836(14)00335-0. doi:, 10.1016/j.jmb.2014.06.019. PMID:25038530<ref>PMID:25038530</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 4q1s" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Proteasome 3D structures|Proteasome 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Saccharomyces cerevisiae S288C]] | ||
| + | [[Category: Beck P]] | ||
| + | [[Category: Elnakady Y]] | ||
| + | [[Category: Groll M]] | ||
| + | [[Category: Heinemeyer W]] | ||
| + | [[Category: Mueller R]] | ||
| + | [[Category: Spaeth A]] | ||
Current revision
Yeast 20S proteasome in Complex with Kendomycin
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