Resolvase

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<StructureSection load='1kcf' size='340' side='right' caption='Yeast Holliday junction resolvase dimer complex with sulfate, [[1kcf]]' scene='' >
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== Function ==
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[[Resolvase]] or '''recombinase''' (Rec) is a nuclease which is involved in DNA recombination. According to the binding residue, the recombinases are grouped to Tyr- and Ser-recombinase.
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{{STRUCTURE_1kcf| PDB=1kcf | SIZE=300| SCENE=Resolvase/Cv/1 |right|CAPTION=Yeast Holliday junction resolvase dimer complex with sulfate, [[1kcf]] }}
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*'''Holliday junction resolvase''' (HJR) resolves 4-way DNA intermediates known as Holliday junctions. Recombination of 2 DNA sites occurs when recombinase binds to the 2 strands<ref>PMID:19442245</ref>. <br />
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[[Resolvase]] or '''recombinase''' (Rec) is a nuclease which is involved in DNA recombination. The '''Holliday junction resolvase''' (HJR) resolves 4-way DNA intermediates known as Holliday junctions. Recombination of 2 DNA sites occurs when recombinase binds to the 2 strands. According to the binding residue, the recombinases are grouped to Tyr- and Ser-recombinase. '''Tyr-recombinases''' include Cre which recombines loxP sites, FLP and lambda integrase (LamInt). '''Ser-recombinases''' (S-rec) include gamma-delta resolvase (GDR), Tn3 resolvase and phiC31 integrase. '''RadA recombinase''' promotes DNA recombination. The images at the left and at the right correspond to one representative resolvase structure, ''i.e.'' the crystal structure of Ydc2 (also known as SpCce1), a Holliday junction resolvase from the fission yeast ''Schizosaccharomyces pombe'' that is involved in the maintenance of mitochondrial DNA ([[1kcf]]). Two sulfate ions are shown as space filling objects. Detailed analysis of its <scene name='Resolvase/Cv/2'>dimeric structure</scene> suggests mechanisms for junction isomerization and communication between the two active sites <ref>PMID:11726496</ref>.
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*'''Tyr-recombinases''' include '''Cre''' which recombines loxP sites, FLP and lambda integrase (LamInt). <br />
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*'''Ser-recombinases''' (S-rec) include gamma-delta resolvase (GDR), Tn3 resolvase and phiC31 integrase.<br />
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*'''RadA recombinase''' promotes DNA recombination. Detailed analysis of its <scene name='Resolvase/Cv/2'>dimeric structure</scene> suggests mechanisms for junction isomerization and communication between the two active sites <ref>PMID:19143611</ref>. <br />
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*'''RadB recombinase''' is a paralogue of RadA assisting in RadA-mediated strand exchange <ref>PMID:28501701</ref>. <br />
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*'''Hin-recombinase''' (HRec) is a protein of ''Salmonella''. HRec inverts a 900 base pair DNA segment which contains the promoters of flagellar genes. The inversion changes the these genes' expressions<ref>PMID:15454079</ref>.
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*'''Tre recombinase''' recognizes a target sequence of the HIV virus resulting in excision and eradication of the provirus from infected human cells <ref>PMID:19066582</ref>. <br />
==3D structures of resolvase==
==3D structures of resolvase==
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[[Resolvase 3D structures]]
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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</StructureSection>
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=== Holliday junction resolvase ===
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[[2wiw]], [[2wiz]], [[2wj0]] – AfHJR+DNA – ''Archaeoglobus fulgidus''<br />
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[[2wcw]], [[2wcz]] – AfHJR (mutant)<br />
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[[2h8c]] – EcHJR RusA (mutant) + DNA – ''Escherichia coli''<br />
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[[1gdt]] - EcHJR +DNA<br />
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[[2h8e]] - EcHJR RusA (mutant)<br />
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[[1q8r]] – EcHJR RusA<br />
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[[1hjr]] – EcHJR RuvC<br />
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[[4ep4]] – TtHJR RuvC – ''Thermus thermophilus''<br />
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[[4ep5]] – TtHJR RuvC (mutant)<br />
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[[1zp7]] – HJR RecU – ''Bacillus subtilis''<br />
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[[1ob8]], [[1ob9]], [[1hh1]] – SsHJR – ''Sulfolobus solfataricus''<br />
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[[2fco]] – HJR – ''Geobacillus kaustophilus''<br />
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[[1ipi]], [[1gef]] – HJR – ''Pyrococcus furiosus''<br />
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[[1kcf]] – HJR – fission yeast<br />
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=== Ser-recombinase ===
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[[2rsl]] - EcGDR<br />
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[[1hx7]] – EcGDR N-terminal – NMR<br />
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[[1ght]], [[1res]], [[1ret]], [[1ght]] – EcGDR catalytic domain – NMR<br />
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[[1gdr]], [[1hx7]] - EcGDR catalytic domain<br />
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[[2gm4]], [[1zr2]], [[1zr4]], [[1gdt]] – EcGDR+DNA<br />
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[[2gm5]] – EcGDR (mutant)<br />
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[[3guv]] – site-specific recombinase – ''Streptococcus pneumoniae''<br />
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[[2r0q]] – S-rec+DNA – ''Staphylococcus aureus''<br />
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[[3lhf]] – SsS-rec<br />
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=== Tyr-recombinase ===
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[[3etl]], [[3ew9]], [[3ewa]], [[2i1q]] – MmRadA Rec+AMPPNP+ion – ''Methanococcus maripaludis''<br />
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[[2b21]] - MmRadA Rec+AMPPNP<br />
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[[2zub]], [[2zuc]], [[2zud]], [[2dfl]] – SsRadA<br />
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[[2cvf]], [[2cvh]] – RadB Rec – ''Thermococcus kodakarensis''<br />
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[[2f1h]], [[2fpl]], [[2fpm]] - MvRec+AMPPNP+K – ''Methanococcus voltae''<br />
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[[2f1i]] – MvRec+AMPPNP<br />
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[[2f1j]], [[2fpk]] - MvRec+ADP<br />
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[[1a0p]] – EcXerd Rec<br />
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=== Cre recombinase ===
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[[3mgv]], [[3c28]], [[3c29]], [[2hof]], [[2hoi]], [[1xns]], [[1xo0]], [[1pvp]], [[1pvq]], [[1pvr]], [[1nzb]], [[1ouq]], [[1q3u]], [[1q3v]], [[1ma7]], [[1kbu]], [[1drg]], [[1f44]], [[2crx]], [[3crx]], [[4crx]], [[5crx]], [[1crx]] – EpCre+DNA – Enterobacteria phage PI<br />
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=== Integrase ===
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[[2a3v]] – Rec INTI4+DNA – ''Vibrio cholerae''<br />
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[[3bvp]] – Integrase N-terminal – Lactococcus phage<br />
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[[2oxo]] – LamInt binding domain – phage<br />
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[[1z19]] – EpLamInt core binding +catalytic domain+DNA<br />
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[[1z1b]], [[1z1g]], [[1p7d]] – EpLamInt+DNA<br />
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[[1kjk]] – EpLamInt N-terminal – NMR<br />
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[[1ae9]] – EpLamInt catalytic core<br />
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[[1p4e]], [[1m6x]] – yFLP (mutant)+DNA – yeast<br />
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[[1flo]] - yFLP +DNA<br />
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[[1aih]] – HP1 integrase catalytic domain - bacteriophage<br />
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===Recombinase A (RecA)===
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[[Recombinase A]]
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==References==
==References==

Current revision

Yeast Holliday junction resolvase dimer complex with sulfate, 1kcf

Drag the structure with the mouse to rotate

References

  1. ↑ West SC. The search for a human Holliday junction resolvase. Biochem Soc Trans. 2009 Jun;37(Pt 3):519-26. doi: 10.1042/BST0370519. PMID:19442245 doi:http://dx.doi.org/10.1042/BST0370519
  2. ↑ Haldenby S, White MF, Allers T. RecA family proteins in archaea: RadA and its cousins. Biochem Soc Trans. 2009 Feb;37(Pt 1):102-7. doi: 10.1042/BST0370102. PMID:19143611 doi:http://dx.doi.org/10.1042/BST0370102
  3. ↑ Wardell K, Haldenby S, Jones N, Liddell S, Ngo GHP, Allers T. RadB acts in homologous recombination in the archaeon Haloferax volcanii, consistent with a role as recombination mediator. DNA Repair (Amst). 2017 Jul;55:7-16. PMID:28501701 doi:10.1016/j.dnarep.2017.04.005
  4. ↑ Dhar G, Sanders ER, Johnson RC. Architecture of the hin synaptic complex during recombination: the recombinase subunits translocate with the DNA strands. Cell. 2004 Oct 1;119(1):33-45. PMID:15454079 doi:http://dx.doi.org/10.1016/j.cell.2004.09.010
  5. ↑ Buchholz F. Molecular evolution of the tre recombinase. J Vis Exp. 2008 May 29;(15):791. PMID:19066582 doi:10.3791/791

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Michal Harel, Alexander Berchansky, Joel L. Sussman, Jaime Prilusky

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