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Resolvase
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| - | + | <StructureSection load='1kcf' size='340' side='right' caption='Yeast Holliday junction resolvase dimer complex with sulfate, [[1kcf]]' scene='' > | |
== Function == | == Function == | ||
[[Resolvase]] or '''recombinase''' (Rec) is a nuclease which is involved in DNA recombination. According to the binding residue, the recombinases are grouped to Tyr- and Ser-recombinase. | [[Resolvase]] or '''recombinase''' (Rec) is a nuclease which is involved in DNA recombination. According to the binding residue, the recombinases are grouped to Tyr- and Ser-recombinase. | ||
| - | *'''Holliday junction resolvase''' (HJR) resolves 4-way DNA intermediates known as Holliday junctions. Recombination of 2 DNA sites occurs when recombinase binds to the 2 strands. <br /> | + | *'''Holliday junction resolvase''' (HJR) resolves 4-way DNA intermediates known as Holliday junctions. Recombination of 2 DNA sites occurs when recombinase binds to the 2 strands<ref>PMID:19442245</ref>. <br /> |
*'''Tyr-recombinases''' include Cre which recombines loxP sites, FLP and lambda integrase (LamInt). <br /> | *'''Tyr-recombinases''' include Cre which recombines loxP sites, FLP and lambda integrase (LamInt). <br /> | ||
*'''Ser-recombinases''' (S-rec) include gamma-delta resolvase (GDR), Tn3 resolvase and phiC31 integrase.<br /> | *'''Ser-recombinases''' (S-rec) include gamma-delta resolvase (GDR), Tn3 resolvase and phiC31 integrase.<br /> | ||
| - | *'''RadA recombinase''' promotes DNA recombination. Detailed analysis of its <scene name='Resolvase/Cv/2'>dimeric structure</scene> suggests mechanisms for junction isomerization and communication between the two active sites <ref>PMID: | + | *'''RadA recombinase''' promotes DNA recombination. Detailed analysis of its <scene name='Resolvase/Cv/2'>dimeric structure</scene> suggests mechanisms for junction isomerization and communication between the two active sites <ref>PMID:19143611</ref>. <br /> |
| - | *'''Hin-recombinase''' (HRec) is a protein of ''Salmonella''. HRec inverts a 900 base pair DNA segment which contains the promoters of flagellar genes. The inversion changes the these genes' expressions. | + | *'''Hin-recombinase''' (HRec) is a protein of ''Salmonella''. HRec inverts a 900 base pair DNA segment which contains the promoters of flagellar genes. The inversion changes the these genes' expressions<ref>PMID:15454079</ref>. |
==3D structures of resolvase== | ==3D structures of resolvase== | ||
| + | [[Resolvase 3D structures]] | ||
| - | + | </StructureSection> | |
| - | + | ||
| - | * Holliday junction resolvase | ||
| - | |||
| - | **[[2wiw]], [[2wiz]], [[2wj0]] – AfHJR+DNA – ''Archaeoglobus fulgidus''<br /> | ||
| - | **[[2wcw]], [[2wcz]] – AfHJR (mutant)<br /> | ||
| - | **[[2h8c]] – EcHJR RusA (mutant) + DNA – ''Escherichia coli''<br /> | ||
| - | **[[1gdt]] - EcHJR +DNA<br /> | ||
| - | **[[2h8e]] - EcHJR RusA (mutant)<br /> | ||
| - | **[[1q8r]] – EcHJR RusA<br /> | ||
| - | **[[1hjr]] – EcHJR RuvC<br /> | ||
| - | **[[4ep4]] – TtHJR RuvC – ''Thermus thermophilus''<br /> | ||
| - | **[[4ep5]] – TtHJR RuvC (mutant)<br /> | ||
| - | **[[1zp7]] – HJR RecU – ''Bacillus subtilis''<br /> | ||
| - | **[[1ob8]], [[1ob9]], [[1hh1]] – SsHJR – ''Sulfolobus solfataricus''<br /> | ||
| - | **[[2fco]] – HJR – ''Geobacillus kaustophilus''<br /> | ||
| - | **[[1ipi]], [[1gef]] – HJR – ''Pyrococcus furiosus''<br /> | ||
| - | **[[1kcf]] – HJR – fission yeast<br /> | ||
| - | |||
| - | * Ser-recombinase | ||
| - | |||
| - | **[[2rsl]] - EcGDR<br /> | ||
| - | **[[1hx7]] – EcGDR N-terminal – NMR<br /> | ||
| - | **[[1ght]], [[1res]], [[1ret]], [[1ght]] – EcGDR catalytic domain – NMR<br /> | ||
| - | **[[1gdr]], [[1hx7]] - EcGDR catalytic domain<br /> | ||
| - | **[[2gm4]], [[1zr2]], [[1zr4]], [[1gdt]] – EcGDR+DNA<br /> | ||
| - | **[[2gm5]] – EcGDR (mutant)<br /> | ||
| - | **[[3guv]] – site-specific recombinase – ''Streptococcus pneumoniae''<br /> | ||
| - | **[[2r0q]] – S-rec+DNA – ''Staphylococcus aureus''<br /> | ||
| - | **[[3lhf]] – SsS-rec<br /> | ||
| - | |||
| - | * Tyr-recombinase | ||
| - | |||
| - | **[[3etl]], [[3ew9]], [[3ewa]], [[2i1q]] – MmRadA Rec+AMPPNP+ion – ''Methanococcus maripaludis''<br /> | ||
| - | **[[2b21]] - MmRadA Rec+AMPPNP<br /> | ||
| - | **[[2zub]], [[2zuc]], [[2zud]], [[2dfl]] – SsRadA<br /> | ||
| - | **[[2cvf]], [[2cvh]] – RadB Rec – ''Thermococcus kodakarensis''<br /> | ||
| - | **[[2f1h]], [[2fpl]], [[2fpm]] - MvRec+AMPPNP+K – ''Methanococcus voltae''<br /> | ||
| - | **[[2f1i]] – MvRec+AMPPNP<br /> | ||
| - | **[[2f1j]], [[2fpk]] - MvRec+ADP<br /> | ||
| - | **[[1a0p]] – EcXerd Rec<br /> | ||
| - | |||
| - | * Cre recombinase | ||
| - | |||
| - | **[[3mgv]], [[3c28]], [[3c29]], [[2hof]], [[2hoi]], [[1xns]], [[1xo0]], [[1pvp]], [[1pvq]], [[1pvr]], [[1nzb]], [[1ouq]], [[1q3u]], [[1q3v]], [[1ma7]], [[1kbu]], [[1drg]], [[1f44]], [[2crx]], [[3crx]], [[4crx]], [[5crx]], [[1crx]] – EpCre+DNA – Enterobacteria phage PI<br /> | ||
| - | |||
| - | *Hin recombinase | ||
| - | |||
| - | **[[1hcr]], [[1ijw]], [[1ijj6]], [[1jj8]], [[1jko]], [[1jkp]], [[1jkq]], [[1jkr]] – HRec + DNA – synthetic <br /> | ||
| - | |||
| - | * Integrase | ||
| - | |||
| - | **[[2a3v]] – Rec INTI4+DNA – ''Vibrio cholerae''<br /> | ||
| - | **[[3bvp]] – Integrase N-terminal – Lactococcus phage<br /> | ||
| - | **[[2oxo]] – LamInt binding domain – phage<br /> | ||
| - | **[[1z19]] – EpLamInt core binding +catalytic domain+DNA<br /> | ||
| - | **[[1z1b]], [[1z1g]], [[1p7d]] – EpLamInt+DNA<br /> | ||
| - | **[[1kjk]] – EpLamInt N-terminal – NMR<br /> | ||
| - | **[[1ae9]] – EpLamInt catalytic core<br /> | ||
| - | **[[1p4e]], [[1m6x]] – yFLP (mutant)+DNA – yeast<br /> | ||
| - | **[[1flo]] - yFLP +DNA<br /> | ||
| - | **[[1aih]] – HP1 integrase catalytic domain - bacteriophage<br /> | ||
| - | **[[3nrw]] – INT/site-specific recombinase N-terminal – ''Haloarcula marismortui''<br /> | ||
| - | **[[3jtz]] – INT arm-type binding domain – ''Yersinia pestis''<br /> | ||
| - | **[[3ju0]] – HPI INT arm-type binding domain – ''Pectobacterium atrosepticum''<br /> | ||
| - | **[[2kkp]] – INT SAM-like domain – ''Moorella thermoacetica'' – NMR<br /> | ||
| - | **[[2kkv]] – INT fragment – ''Salmonella enterica'' – NMR<br /> | ||
| - | **[[2khq]] - INT fragment – ''Staphylococcus saprophyticus'' – NMR<br /> | ||
| - | **[[2wcc]] – λINT DBD+ DNA – Enterobacteria phage λ - NMR<br /> | ||
| - | **[[2khv]], [[2kj5]] – INT residues 102-199 – ''Nitrosospira multiformis'' – NMR<br /> | ||
| - | |||
| - | *Recombinase A (RecA) see [[Recombinase A]] | ||
| - | }} | ||
==References== | ==References== | ||
<references /> | <references /> | ||
[[Category:Topic Page]] | [[Category:Topic Page]] | ||
Current revision
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References
- ↑ West SC. The search for a human Holliday junction resolvase. Biochem Soc Trans. 2009 Jun;37(Pt 3):519-26. doi: 10.1042/BST0370519. PMID:19442245 doi:http://dx.doi.org/10.1042/BST0370519
- ↑ Haldenby S, White MF, Allers T. RecA family proteins in archaea: RadA and its cousins. Biochem Soc Trans. 2009 Feb;37(Pt 1):102-7. doi: 10.1042/BST0370102. PMID:19143611 doi:http://dx.doi.org/10.1042/BST0370102
- ↑ Dhar G, Sanders ER, Johnson RC. Architecture of the hin synaptic complex during recombination: the recombinase subunits translocate with the DNA strands. Cell. 2004 Oct 1;119(1):33-45. PMID:15454079 doi:http://dx.doi.org/10.1016/j.cell.2004.09.010
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