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2z4d

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NMR Structures of Yeast Proteasome Component Rpn13

Structural highlights

2z4d is a 1 chain structure with sequence from Saccharomyces cerevisiae. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RPN13_YEAST Component of the 19S cap proteasome complex which acts as a regulatory subunit of the 26S proteasome, involved in the ATP-dependent degradation of ubiquitinated proteins.^[1] ^[2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Proteasomal receptors that recognize ubiquitin chains attached to substrates are key mediators of selective protein degradation in eukaryotes. Here we report the identification of a new ubiquitin receptor, Rpn13/ARM1, a known component of the proteasome. Rpn13 binds ubiquitin through a conserved amino-terminal region termed the pleckstrin-like receptor for ubiquitin (Pru) domain, which binds K48-linked diubiquitin with an affinity of approximately 90 nM. Like proteasomal ubiquitin receptor Rpn10/S5a, Rpn13 also binds ubiquitin-like (UBL) domains of UBL-ubiquitin-associated (UBA) proteins. In yeast, a synthetic phenotype results when specific mutations of the ubiquitin binding sites of Rpn10 and Rpn13 are combined, indicating functional linkage between these ubiquitin receptors. Because Rpn13 is also the proteasomal receptor for Uch37, a deubiquitinating enzyme, our findings suggest a coupling of chain recognition and disassembly at the proteasome.

Proteasome subunit Rpn13 is a novel ubiquitin receptor.,Husnjak K, Elsasser S, Zhang N, Chen X, Randles L, Shi Y, Hofmann K, Walters KJ, Finley D, Dikic I Nature. 2008 May 22;453(7194):481-8. PMID:18497817^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Fischer M, Hilt W, Richter-Ruoff B, Gonen H, Ciechanover A, Wolf DH. The 26S proteasome of the yeast Saccharomyces cerevisiae. FEBS Lett. 1994 Nov 21;355(1):69-75. PMID:7957966
↑ Verma R, Chen S, Feldman R, Schieltz D, Yates J, Dohmen J, Deshaies RJ. Proteasomal proteomics: identification of nucleotide-sensitive proteasome-interacting proteins by mass spectrometric analysis of affinity-purified proteasomes. Mol Biol Cell. 2000 Oct;11(10):3425-39. PMID:11029046
↑ Husnjak K, Elsasser S, Zhang N, Chen X, Randles L, Shi Y, Hofmann K, Walters KJ, Finley D, Dikic I. Proteasome subunit Rpn13 is a novel ubiquitin receptor. Nature. 2008 May 22;453(7194):481-8. PMID:18497817 doi:10.1038/nature06926

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2z4d"

Categories: Large Structures | Saccharomyces cerevisiae | Walters KJ | Zhang N

@@ Line 1: / Line 1: @@
 ==NMR Structures of Yeast Proteasome Component Rpn13==
-<StructureSection load='2z4d' size='340' side='right' caption='[[2z4d]], [[NMR_Ensembles_of_Models | 8 NMR models]]' scene=''>
+<StructureSection load='2z4d' size='340' side='right'caption='[[2z4d]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2z4d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z4D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2Z4D FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2z4d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z4D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Z4D FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ubq|1ubq]], [[2coc|2coc]], [[2dhk|2dhk]], [[2hth|2hth]], [[1fho|1fho]], [[2d9v|2d9v]]</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RPN13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2z4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z4d OCA], [https://pdbe.org/2z4d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2z4d RCSB], [https://www.ebi.ac.uk/pdbsum/2z4d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2z4d ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2z4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z4d OCA], [http://pdbe.org/2z4d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2z4d RCSB], [http://www.ebi.ac.uk/pdbsum/2z4d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2z4d ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/RPN13_YEAST RPN13_YEAST]] Component of the 19S cap proteasome complex which acts as a regulatory subunit of the 26S proteasome, involved in the ATP-dependent degradation of ubiquitinated proteins.<ref>PMID:7957966</ref> <ref>PMID:11029046</ref>
+[https://www.uniprot.org/uniprot/RPN13_YEAST RPN13_YEAST] Component of the 19S cap proteasome complex which acts as a regulatory subunit of the 26S proteasome, involved in the ATP-dependent degradation of ubiquitinated proteins.<ref>PMID:7957966</ref> <ref>PMID:11029046</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z4/2z4d_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z4/2z4d_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 31: / Line 30: @@
 ==See Also==
-*[[Proteasome|Proteasome]]
+*[[Proteasome 3D structures|Proteasome 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Atcc 18824]]
+[[Category: Large Structures]]
-[[Category: Walters, K J]]
+[[Category: Saccharomyces cerevisiae]]
-[[Category: Zhang, N]]
+[[Category: Walters KJ]]
-[[Category: Nuclear protein]]
+[[Category: Zhang N]]
-[[Category: Ph domain]]
-[[Category: Proteasome]]

2z4d

From Proteopedia

Current revision

NMR Structures of Yeast Proteasome Component Rpn13

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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