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| | ==Structural and functional studies of the trans-encoded HLA-DQ2.3 (DQA1*03:01/DQB1*02:01) molecule== | | ==Structural and functional studies of the trans-encoded HLA-DQ2.3 (DQA1*03:01/DQB1*02:01) molecule== |
| - | <StructureSection load='4d8p' size='340' side='right' caption='[[4d8p]], [[Resolution|resolution]] 3.05Å' scene=''> | + | <StructureSection load='4d8p' size='340' side='right'caption='[[4d8p]], [[Resolution|resolution]] 3.05Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4d8p]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Canadian_hard_winter_wheat Canadian hard winter wheat] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D8P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4D8P FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4d8p]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D8P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4D8P FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.05Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HLA-DQA1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), HLA-DQB1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4565 Canadian hard winter wheat])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4d8p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d8p OCA], [http://pdbe.org/4d8p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4d8p RCSB], [http://www.ebi.ac.uk/pdbsum/4d8p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4d8p ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4d8p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d8p OCA], [https://pdbe.org/4d8p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4d8p RCSB], [https://www.ebi.ac.uk/pdbsum/4d8p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4d8p ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/L8E864_HUMAN L8E864_HUMAN] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Canadian hard winter wheat]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Human]] | + | [[Category: Large Structures]] |
| - | [[Category: Chen, X]] | + | [[Category: Triticum aestivum]] |
| - | [[Category: Hotta, K]] | + | [[Category: Chen X]] |
| - | [[Category: Kim, C Y]] | + | [[Category: Hotta K]] |
| - | [[Category: Mathews, I I]] | + | [[Category: Kim C-Y]] |
| - | [[Category: Class ii mhc]]
| + | [[Category: Mathews II]] |
| - | [[Category: Immune system]]
| + | |
| Structural highlights
Function
L8E864_HUMAN
Publication Abstract from PubMed
MHC class II molecules are composed of one alpha-chain and one beta-chain whose membrane distal interface forms the peptide binding groove. Most of the existing knowledge on MHC class II molecules comes from the cis-encoded variants where the alpha- and beta-chain are encoded on the same chromosome. However, trans-encoded class II MHC molecules, where the alpha- and beta-chain are encoded on opposite chromosomes, can also be expressed. We have studied the trans-encoded class II HLA molecule DQ2.3 (DQA1*03:01/DQB1*02:01) that has received particular attention as it may explain the increased risk of certain individuals to type 1 diabetes. We report the X-ray crystal structure of this HLA molecule complexed with a gluten epitope at 3.05 A resolution. The gluten epitope is preferentially recognized in context of the DQ2.3 molecule by T-cell clones of a DQ8/DQ2.5 heterozygous celiac disease patient. This preferential recognition can be explained by improved HLA binding as the epitope combines the peptide binding motif of DQ2.5 (negative charge at P4) and DQ8 (negative charge at P1). The analysis of the structure of DQ2.3 together with all other available DQ crystal structures and sequences led us to categorize DQA1 and DQB1 genes into two groups where any alpha-chain and beta-chain belonging to the same group are expected to form a stable heterodimer.
Structural and functional studies of the trans-encoded HLA-DQ2.3 (DQA1*03:01/DQB1*02:01) molecule.,Tollefsen S, Hotta K, Chen X, Simonsen B, Swaminathan K, Mathews II, Sollid LM, Kim CY J Biol Chem. 2012 Feb 23. PMID:22362761[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Tollefsen S, Hotta K, Chen X, Simonsen B, Swaminathan K, Mathews II, Sollid LM, Kim CY. Structural and functional studies of the trans-encoded HLA-DQ2.3 (DQA1*03:01/DQB1*02:01) molecule. J Biol Chem. 2012 Feb 23. PMID:22362761 doi:10.1074/jbc.M111.320374
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