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Spermidine/spermine N-acetyltransferase
From Proteopedia
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| - | + | <StructureSection load='' size='350' side='right' scene='48/486363/Cv/2' caption='Human SSAT1 dimer complex with acetyl-spermine-S-CoA, [[2jev]]'> | |
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== Function == | == Function == | ||
| - | '''Spermidine/spermine N-acetyltransferase''' (SSAT) catalyzes the acetylation of spermidine and spermine. SSAT regulates cellular polyamine homeostasis by degrading polyamines via their acetylation. SSAT activity is regulated by polyamine concentration and various toxins, hormones and natural products<ref>PMID:18349109</ref>. | + | '''Spermidine/spermine N-acetyltransferase''' (SSAT) or '''Spermidine N-acetyltransferase''' or '''diamine acetyltransferase''' catalyzes the acetylation of spermidine and spermine. SSAT regulates cellular polyamine homeostasis by degrading polyamines via their acetylation. SSAT activity is regulated by polyamine concentration and various toxins, hormones and natural products<ref>PMID:18349109</ref>. |
== Relevance == | == Relevance == | ||
Several types of cancer are accompanied by increased level of cellular polyamine and compounds which affect SSAT activity are explored as possible anti-cancer drugs<ref>PMID:17237273</ref>. | Several types of cancer are accompanied by increased level of cellular polyamine and compounds which affect SSAT activity are explored as possible anti-cancer drugs<ref>PMID:17237273</ref>. | ||
| + | == Structural highlights == | ||
| + | The <scene name='48/486363/Cv/7'>active site of SSAT contains a bisubstrate inhibitor</scene>. In <scene name='48/486363/Cv/8'>catalysis, residues Tyr140 act as general acid and Glu92 as general base</scene><ref>PMID:17516632</ref> (colored in cyan). Water molecules are shown as red spheres. | ||
| + | </StructureSection> | ||
==3D structures of SSAT== | ==3D structures of SSAT== | ||
{{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | ||
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**[[2q4v]], [[2bei]] – hSSAT2 – human<br /> | **[[2q4v]], [[2bei]] – hSSAT2 – human<br /> | ||
**[[2f5i]], [[2b5g]], [[2g3t]] – hSSAT1<br /> | **[[2f5i]], [[2b5g]], [[2g3t]] – hSSAT1<br /> | ||
| - | **[[2b3u]] – hSSAT1 (mutant) | + | **[[2b3u]] – hSSAT1 (mutant)<br /> |
| + | **[[2fl4]] – SSAT – ''Enteococcus faecalis''<br /> | ||
*Spermidine/spermine N-acetyltransferase complexes | *Spermidine/spermine N-acetyltransferase complexes | ||
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**[[2b4d]], [[2b58]], [[3bj7]] - hSSAT1 + CoA<br /> | **[[2b4d]], [[2b58]], [[3bj7]] - hSSAT1 + CoA<br /> | ||
**[[2jev]] - hSSAT1 + acetylspermine-S-CoA<br /> | **[[2jev]] - hSSAT1 + acetylspermine-S-CoA<br /> | ||
| - | **[[3bj8]] - hSSAT1 + spermine + CoA | + | **[[3bj8]] - hSSAT1 + spermine + CoA<br /> |
| + | **[[6yug]] – SSAT + CoA + G6P – ''Cryptosporidium parvum''<br /> | ||
| + | |||
| + | *Spermidine/spermine N-acetyltransferase SpeG | ||
| + | |||
| + | **[[5cnp]], [[5ug4]], [[6cx8]], [[6e1x]], [[4jjx]], [[4jly]], [[4ygo]], [[6cx8]] – VcSSAT – ''Vibrio cholerae''<br /> | ||
| + | **[[6dau]], [[7kwx]], [[7kx2]], [[7kx3]] – VcSSAT (mutant)<br /> | ||
| + | **[[7kwh]], [[7kwj]], [[7kwq]] – VcSSAT SpeG/hSSAT<br /> | ||
| + | **[[4r9m]], [[6cy6]] – SSAT – ''Escherichia coli''<br /> | ||
| + | **[[6d72]] – YpSSAT - ''Yersinia pestis''<br /> | ||
| + | **[[4mi4]] – VcSSAT + spermine <br /> | ||
| + | **[[4mhd]] – VcSSAT + spermidine <br /> | ||
| + | **[[4r57]] – VcSSAT + CoA <br /> | ||
| + | **[[4mj8]] – VcSSAT + polyamine <br /> | ||
| + | **[[4r87]] – VcSSAT + spermine + CoA <br /> | ||
| + | **[[4ncz]] – VcSSAT + sulfonate derivative<br /> | ||
| + | **[[5wif]] – YpSSAT + methoxy-methoxy–ethoxy-ethane <br /> | ||
}} | }} | ||
== References == | == References == | ||
<references/> | <references/> | ||
[[Category:Topic Page]] | [[Category:Topic Page]] | ||
Current revision
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3D structures of SSAT
07-April-2022
References
- ↑ Pegg AE. Spermidine/spermine-N(1)-acetyltransferase: a key metabolic regulator. Am J Physiol Endocrinol Metab. 2008 Jun;294(6):E995-1010. doi:, 10.1152/ajpendo.90217.2008. Epub 2008 Mar 18. PMID:18349109 doi:http://dx.doi.org/10.1152/ajpendo.90217.2008
- ↑ Allen WL, McLean EG, Boyer J, McCulla A, Wilson PM, Coyle V, Longley DB, Casero RA Jr, Johnston PG. The role of spermidine/spermine N1-acetyltransferase in determining response to chemotherapeutic agents in colorectal cancer cells. Mol Cancer Ther. 2007 Jan;6(1):128-37. PMID:17237273 doi:http://dx.doi.org/10.1158/1535-7163.MCT-06-0303
- ↑ Hegde SS, Chandler J, Vetting MW, Yu M, Blanchard JS. Mechanistic and structural analysis of human spermidine/spermine N1-acetyltransferase. Biochemistry. 2007 Jun 19;46(24):7187-95. Epub 2007 May 22. PMID:17516632 doi:10.1021/bi700256z
