User:Fadel A. Samatey/FlgE I
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< User:Fadel A. Samatey(Difference between revisions)
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| - | <span style="border: 2px solid #a1a1a1; border-radius: 6px;padding:35px 50px 20px 30px;background: linear-gradient(#b0d0ff, #ffffff);"><span style="font-size:250%;">Scientific Reports</span> | + | <span style="border: 2px solid #a1a1a1; border-radius: 6px;padding:35px 50px 20px 30px;background: linear-gradient(#b0d0ff, #ffffff);white-space:nowrap;"><span style="font-size:250%;">Scientific Reports</span> |
<span style="padding: 0px 0px 0px 100px;color:#808080;font-size:120%;">an online, open access journal: [http://www.nature.com/srep nature.com/srep]</span></span> | <span style="padding: 0px 0px 0px 100px;color:#808080;font-size:120%;">an online, open access journal: [http://www.nature.com/srep nature.com/srep]</span></span> | ||
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</span> | </span> | ||
<br><br> | <br><br> | ||
| - | The interactive Molecular Tour below assumes that you are familiar with the journal article. | + | The interactive Molecular Tour below assumes that you are familiar with the journal article<ref name="s3">PMID: 27759043</ref>. |
==Molecular Tour== | ==Molecular Tour== | ||
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==Comparison with ''Salmonella''== | ==Comparison with ''Salmonella''== | ||
| - | FlgE (gene STM1177) of ''Salmonella enterica'' serotype Typhimurium (FlgE-St, [http://www.uniprot.org/uniprot/P0A1J1 P0A1J1_SALTY]) contains 403 amino acids, compared to the 865 in FlgE-Cj. However, the D1 and D2 domains are very similar in the two species (FlgE-St42: [[1wlg]]). | + | FlgE (gene STM1177) of ''Salmonella enterica'' serotype Typhimurium<ref>PMID: 10878026</ref> (FlgE-St, [http://www.uniprot.org/uniprot/P0A1J1 P0A1J1_SALTY]) contains 403 amino acids, compared to the 865 in FlgE-Cj. However, the D1 and D2 domains are very similar in the two species (FlgE-St42: [[1wlg]]). |
<center> | <center> | ||
This animation takes ~2 minutes. Reload the page to cancel. | This animation takes ~2 minutes. Reload the page to cancel. | ||
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Each of the 11 protofilaments has a distinct color, so there are 11 colors. Here, <scene name='47/478822/Caucr-hook-cao/2'>one protofilament is marked with a black line</scene>. Each protofilament forms one of the 11-start helices. | Each of the 11 protofilaments has a distinct color, so there are 11 colors. Here, <scene name='47/478822/Caucr-hook-cao/2'>one protofilament is marked with a black line</scene>. Each protofilament forms one of the 11-start helices. | ||
| - | There is a large space between domains D2+D3 '''within''' a protofilament. Thus, protofilaments are held together by interactions between D0 and D1 (missing in this model). However, domains D2+D3 interact '''between''' protofilaments, forming 6 helices. Here, <scene name='47/478822/Caucr-hook-cao/3'>one of the 6-start helices is marked with a red line, and the 6 starts are numbered</scene>. | + | There is a large space between domains D2+D3 '''within''' a protofilament. Thus, protofilaments are held together by interactions between D0 and D1 (missing in this model). However, domains D2+D3 interact '''between''' protofilaments, forming 6 helices. Here, <scene name='47/478822/Caucr-hook-cao/3'>one of the 6-start helices is marked with a red line, and the 6 starts are numbered</scene>.<b> |
| - | + | *Black line: one of the 11-start protofilament helices. <font color="red"> | |
| + | *Red line: one of the 6-start helices.</font></b> | ||
<hr> | <hr> | ||
</StructureSection> | </StructureSection> | ||
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| + | ==See Also== | ||
| + | * [[The Bacterial Flagellar Hook]] | ||
| + | * [[User:Fadel A. Samatey/FlgE II/Complete Flagellar Hook Structure|Cryo-EM structure of the complete flagellar hook of ''Campylobacter jejuni'']] (2016) | ||
==Notes and References== | ==Notes and References== | ||
<references /> | <references /> | ||
Current revision
Interactive 3D Complement in Proteopedia
Scientific Reports an online, open access journal: nature.com/srep
Structural insights into bacterial flagellar hook similarities and specifities.
Young-Ho Yoon, Clive S. Barker, Paula V. Bulieris, Hideyuki Matsunami, and Fadel A. Samatey.
Scientific Reports 6:35552, 2016: nature.com/articles/srep35552. (DOI: 10.1038/srep35552)
The interactive Molecular Tour below assumes that you are familiar with the journal article[1].
Molecular Tour
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See Also
- The Bacterial Flagellar Hook
- Cryo-EM structure of the complete flagellar hook of Campylobacter jejuni (2016)
Notes and References
- ↑ Yoon YH, Barker CS, Bulieris PV, Matsunami H, Samatey FA. Structural insights into bacterial flagellar hooks similarities and specificities. Sci Rep. 2016 Oct 19;6:35552. doi: 10.1038/srep35552. PMID:27759043 doi:http://dx.doi.org/10.1038/srep35552
- ↑ Kido Y, Yoon YH, Samatey FA. Crystallization of a 79 kDa fragment of the hook protein FlgE from Campylobacter jejuni. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Dec 1;67(Pt, 12):1653-7. Epub 2011 Nov 30. PMID:22139190 doi:10.1107/S1744309111043272
- ↑ Brenner FW, Villar RG, Angulo FJ, Tauxe R, Swaminathan B. Salmonella nomenclature. J Clin Microbiol. 2000 Jul;38(7):2465-7. PMID:10878026
- ↑ Wagenknecht T, DeRosier D, Shapiro L, Weissborn A. Three-dimensional reconstruction of the flagellar hook from Caulobacter crescentus. J Mol Biol. 1981 Sep 25;151(3):439-65. PMID:7338902 doi:http://dx.doi.org/10.1016/0022-2836(81)90005-X
- ↑ The Caulobacter crecentus hook model contains 55 copies of the monomer, for a total of 121,055 non-hydrogen atoms. There are 16,665 alpha carbon atoms in the model. The van der Waals radius of carbon is 1.7 Å. In order to make the FlgE domains look solid, the alpha carbons are displayed with a radius of 3.5 Å.
