The Bacterial Flagellar Hook

From Proteopedia

The bacterial flagellar hook described in this article is one part of the bacterial flagellum. Please see Flagella, bacterial for an overview of where the hook fits in the flagellum.

Function

The flagellar hook is a molecular universal joint that transmits torque from the motor, anchored in the bacterial cell wall, to the flagellar filament, the relatively rigid helical rod that propels the bacterial cell when rotated. The hook is flexible: it enables the filament to adopt a wide range of angles relative to the motor axis and cell wall, yet continue to be rotated by the motor at all these angles.

Structure of the Hook Monomer, FlgE

The hook filament of Salmonella typhimurium is composed of about 120 copies of the protein chain FlgE (the product of the gene flgE), a protein of length 402 amino acids.

FlgE31 2004

In 2004, Samatey et al. solved the structure of the mid-portion of the wild-type FlgE of Salmonella typhimurium by X-ray crystallography at a resolution of 1.8 Å (1wlg)^[1]. The fragment successfully crystallized, designated FlgE31, consisted of amino acids 71-369, of which 71-363 were resolved (72% of the full-length 402-residue protein). Removal of the ends of the full-length chain (domain D0, see below) was required in order to coax the protein to crystallize, instead of forming filaments.

FlgE consists of three domains, D0, D1, and D2. The () at right shows D1 and D2 (D0 having been deleted in order to achieve crystallization, as mentioned above).

Note that D2 consists of a continuous segment of the protein chain, while D1 is made up of discontinuous segments. D0 (not shown, see above, also discontinuous) is attached to D1, and D0 forms the inner channel of the hook. D2 forms the outer surface of the hook.

[Show evolutionary conservation here after ConSurf server is again available.]

Hook Filament Structure

	Bacterial flagellar hook, rotating simulation, used with permission of Fadel A. Samatey.

In the original monomer structure paper^[1], the monomer was fitted into an electron density map of about 10 Å resolution, generated by electron cryomicroscopy from hook filaments of Salmonella typhimurium (see also^[2]). Hook filaments are normally curved (at room temperature), but these hook filaments were rendered straight by cold storage, which facilitated image averaging. The D1 and D2 domains of the monomer were independently docked into the map and then ligated. A model of the curved hook filament was generated using symmetry operations that produced the known radius of curvature. This model was then rotated, producing the simulation shown at right.

In 2009, a higher resolution (7.1 Å) electron density map of the straight hook, from cryomicroscopy, was reported by Fujii, Kato and Namba^[3].

Monomer Within the Hook

From the curved model of the hook, here is a short segment just three Flg31 monomers long (). Each color represents one of the 11 protofilaments. Since each protofilament is 3 monomers in length, there are 33 monomers in this model. You can see how the Flg31 monomers fit into the hook when .

The monomers are slightly larger and more tightly packed than indicated in this model, because this model contains only alpha-carbon atoms. Also the hole in the center of the actual hook filament is much smaller than shown here, because this model lacks the D0 domain that occupies the core of the hook filament.

Content Attribution

The initial content for this article was adapted, with permission, from The Bacterial Flagellar Hook: A Molecular Universal Joint, authored by User:Eric Martz in 2004-2006 for Protein Explorer.

See Also

• Flagella, bacterial
• Cryo-EM structure of the complete flagellar hook of Campylobacter jejuni (2016)
• X-ray structure of the hook monomers of Campylobacter jejuni and also of Caulobacter cresentus (2016)
• Flagellar filament of bacteria
• Fadel A. Samatey Group

3D structures of flagellar hook protein

Updated on 04-September-2021

1wlg – StFlgE31 – Salmonella typhimurium
2bgy, 2bgz - StFlgE31 residues 71-363 – Cryo EM
3a69 - StFlgE31 – Cryo EM
5jxl – FlgE – Campylobacter jejuni
5ay6 – FlgE – Caulobacter crescentus

References and Notes

1. ↑ ^{1.0 1.1} Samatey FA, Matsunami H, Imada K, Nagashima S, Shaikh TR, Thomas DR, Chen JZ, Derosier DJ, Kitao A, Namba K. Structure of the bacterial flagellar hook and implication for the molecular universal joint mechanism. Nature. 2004 Oct 28;431(7012):1062-8. PMID:15510139 doi:http://dx.doi.org/10.1038/nature02997
2. ↑ Shaikh TR, Thomas DR, Chen JZ, Samatey FA, Matsunami H, Imada K, Namba K, Derosier DJ. A partial atomic structure for the flagellar hook of Salmonella typhimurium. Proc Natl Acad Sci U S A. 2005 Jan 25;102(4):1023-8. Epub 2005 Jan 18. PMID:15657146
3. ↑ Fujii T, Kato T, Namba K. Specific arrangement of alpha-helical coiled coils in the core domain of the bacterial flagellar hook for the universal joint function. Structure. 2009 Nov 11;17(11):1485-93. PMID:19913483 doi:10.1016/j.str.2009.08.017